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Non-destructive micro-measurement system that can observe fluorescent biological samples

A non-destructive, fluorescent technology, applied in the field of electrochemistry, can solve the problem of inability to observe the fluorescence of biological samples

Inactive Publication Date: 2012-02-08
CAPITAL NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In order to overcome the disadvantage that the optical imaging system of the existing non-destructive micro-measurement system cannot observe the fluorescence emitted by biological samples, the present invention improves the optical imaging system on the basis of the existing non-destructive micro-measurement system, so that the non-destructive micro-measurement system can While measuring the flow direction of ions / molecules on the surface of biological materials, the fluorescence emitted by biological samples can be observed to achieve the purpose of screening and state monitoring of the tested biological materials

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  • Non-destructive micro-measurement system that can observe fluorescent biological samples
  • Non-destructive micro-measurement system that can observe fluorescent biological samples
  • Non-destructive micro-measurement system that can observe fluorescent biological samples

Examples

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Effect test

example 1

[0028] Example 1: A non-destructive micro-assay system that can observe and sort fluorescent biological samples

[0029] figure 1 The illustrated embodiment is a non-invasive microassay system combined with a fluorescent device and an optical clamp device, which can be used for fluorescent recognition of transformed biological samples labeled with green fluorescent protein (GFP) and optical clamp fixation and movement of yeast cells.

[0030] Among them, bright field illuminator 1-1, fluorescent illuminator 1-2, stage 0-1, objective lens changer 1-8, filter turntable changer 1-3, filter turntable changer 1-4, Structures such as eyepieces 1-11 are provided by manufacturers of commercial products for inverted fluorescence microscopes. A 100x objective lens is installed in the objective lens changer 1-8 to converge the optical clamp laser beam. The optical filter turntable converter 1-3 is installed with: excitation color filter 1-5 wavelength is 450-490 nanometers, limits exci...

Embodiment 2

[0035] Embodiment 2: Application of the present invention to the detection of transgenic plant material

[0036] 1. Transgenic plant material

[0037] GFP fusion protein transgenic Arabidopsis thaliana seedlings germinated for 5 days

[0038] 2. Material Screening and Image Capture by Fluorescence Microscopy

[0039] The roots, root tips, and root hairs of the transgenic plants were clearly observed under a 10X or 40X microscope in bright field. Then switch to blue excitation light to excite the sample, observe and capture the transgenic material with green fluorescent expression. Finally, use the software to capture the image.

[0040] 3. Nondestructive Microtesting

[0041] Including: glass electrode drawing, molecular / ion electrode buffer filling, LIX filling, ion / molecular electrode silver wire chlorination, molecular / ion electrode static calibration and test sample detection. The site detected by the experiment: the root hair area (such as figure 2 ).

[0042] 4. ...

Embodiment 3

[0044] Embodiment 3: The present invention is used for the detection of transgenic tobacco suspension cells

[0045] 1. Culture and fixation of transgenic tobacco suspension cells

[0046] The transgenic tobacco suspension cells that had undergone resistance screening were subcultured once a week with sterile modified MS liquid medium at a ratio of 1:10, and they could be used for detection 3-4 days after subculture. Modified MS medium with newly added KH 2 PO 4 30mg / L, 2,4-D0.6mg / L, VB10.5mg / L MS medium. During the test, 1% low-melting glue was used to fix the transgenic tobacco suspension cells in the test container.

[0047] 2. Material Screening and Image Capture by Fluorescence Microscopy

[0048] The transgenic tobacco suspension cells were clearly observed under a 10X or 40X microscope in bright field. Then switch to blue excitation light to excite the sample, and observe the transgenic tobacco suspension cells expressing green fluorescence as capture objects. Fin...

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Abstract

In order to overcome the disadvantage that the optical imaging system of the existing non-destructive micro-measurement system cannot observe the fluorescence emitted by biological samples, the present invention improves the optical imaging system on the basis of the existing non-destructive micro-measurement system, so that the non-destructive micro-measurement system can While measuring the flow direction of ions / molecules on the surface of biological materials, the fluorescence emitted by biological samples can be observed to achieve the purpose of screening and state monitoring of the tested biological materials. The invention belongs to the field of electrochemical technology, and includes an ion / molecular activity detection system, a microscopic imaging system and a computer data processing system, wherein the microscopic imaging system includes a bright field illuminator, a stage, an objective lens, an objective lens converter, and an optical path switch A filter, an eyepiece and a digital camera are characterized in that a filter turntable switch for fluorescence microscopic observation mode is installed between the light path connecting the objective lens and the light path switcher in the microscopic imaging system, and the filter turntable switch contains excitation Filters, Fluorescent Dichroic Mirrors, and Blocking Filters.

Description

technical field [0001] The invention belongs to the technical field of electrochemistry, and in particular relates to an improved optical electrophysiology system capable of detecting ion / molecular transport flow velocity and direction. The electrophysiological conditions of cells can be observed by applying the invention. Background technique [0002] Substances in a liquid environment have a tendency to diffuse from high to low concentrations. For charged particles there is also a tendency to move from a high electrochemical potential to a low electrochemical potential. [0003] The core of the non-destructive micro-measurement system is the selective ion / molecular microelectrode, hereinafter referred to as the ion / molecular selective electrode, which evolved from a computer-controlled automatic positioning measurement system designed by Kühtreiber and Jaffe in 1990 (Kühtreiber and Jaffe in J. Cell Biol 1990, 110: 1565-1573). The ion selective electrode is used in conjun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48G01N21/64C12Q1/02
Inventor 印莉萍赵伟忠杨光郭家峰靳思房茜张鹏
Owner CAPITAL NORMAL UNIVERSITY
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