Method for refining cholesterin by activated carbon fixed bed adsorption process
A fixed bed adsorption and activated carbon technology, applied in the field of adsorption and separation, can solve the problems of low content, environmental pollution and high cost, and achieve the effects of high yield and content, good decolorization effect and low production cost
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Embodiment 1
[0040] Weigh 100.0 g of a crude product with a cholesterol content of 23.8%, and dissolve it in 1000 ml of methanol at room temperature to prepare a raw material solution. The size of the fixed bed is φ5cm×25cm, and 113g of coarse-pore granular activated carbon is filled inside, and the bed volume (BV) is 490mL. The volume of the raw material liquid prepared above is roughly equivalent to 2BV. The raw material liquid passes through the fixed bed at a flow rate of 0.2BV / h at 40°C, and the cholesterol in the raw material liquid is adsorbed on the activated carbon of the fixed bed.
[0041] Continue to flush the fixed bed with 1.0BV of methanol at a flow rate of 1.0BV / h to recover a small amount of raw material liquid remaining in the gap of the fixed bed, collect the liquid obtained by washing and put it into the raw material liquid for mechanical use.
[0042] At 40°C, use 3.0 BV of acetone to flow through the fixed bed washed with methanol at a flow rate of 1.5 BV / h to elute ...
Embodiment 2
[0045] Weigh 100.0 g of a crude product with a cholesterol content of 23.8%, and dissolve it in 1000 ml of methanol at room temperature to prepare a raw material solution. The size of the fixed bed is φ5cm×25cm, and it is filled with 113g of coarse-pore granular activated carbon that has been used once and regenerated with n-hexane and balanced with methanol. The bed volume (BV) is 490mL. The volume of the raw material liquid prepared above is roughly equivalent to 2BV. The raw material liquid passes through the fixed bed at a flow rate of 0.2BV / h at 40°C, and the cholesterol in the raw material liquid is adsorbed on the activated carbon of the fixed bed.
[0046] Continue to flush the fixed bed with 1.0BV of methanol at a flow rate of 1.0BV / h to recover a small amount of raw material liquid remaining in the gap of the fixed bed, collect the liquid obtained by washing and put it into the raw material liquid for mechanical use.
[0047] At 40°C, use 3.0 BV of acetone to flow t...
Embodiment 3
[0050] Weigh 100.0 g of a crude product with a cholesterol content of 23.8%, and dissolve it in 1000 ml of methanol at room temperature to prepare a raw material solution. The size of the fixed bed is φ5cm×25cm, filled with 113g of granular activated carbon that has been used 10 times and regenerated with n-hexane and balanced with methanol. The bed volume (BV) is 490mL. The volume of the raw material liquid prepared above is roughly equivalent to 2BV. The raw material liquid passes through the fixed bed at a flow rate of 0.2BV / h at 40°C, and the cholesterol in the raw material liquid is adsorbed on the activated carbon of the fixed bed.
[0051] Continue to flush the fixed bed with 1.0BV of methanol at a flow rate of 1.0BV / h to recover a small amount of raw material liquid remaining in the gap of the fixed bed, collect the liquid obtained by washing and put it into the raw material liquid for mechanical use.
[0052] At 40°C, use 3.0 BV of acetone to flow through the fixed b...
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