Functional peptide section of epididymis protease inhibitors and multiple antigenic peptide constructed thereby

Abstract

The invention discloses a functional peptide piece of an epididymal protease inhibitor and a multiplex antigenic peptide constructed by the functional peptide piece. The functional peptide piece of the epididymal protease inhibitor is selected from the amino acid sequence shown by SEQ ID No.1 or SEQ ID No.2, which is characterized by single component, simple structure and pertinent triggered immune response and can be used for preparing immunogen. The complex antigenic peptide constructed by the functional peptide piece is obtained by the covalent connection of the functional peptide piece toa branch of the treelike core formed by one or a plurality of bifunctional units. The multiplex antigenic peptide can improve the immunogenicity of the functional peptide piece without affecting the antigenic specificity, stimulate the organism to generate an antibody which has high-titre and is specific with the functional peptide piece, reduce the level of the serum epididymal protease inhibitor and induce efficient, safe and reversible antifertility action, thus being applicable to preparing male contraceptive vaccine.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a functional peptide segment of an epididymis protease inhibitor and a multiple antigen peptide constructed using the functional peptide segment. Background technique [0002] Population, resource and environmental issues are key issues that seriously affect human survival and economic and social development in this century, and research on contraception has received great attention. So far, male contraceptive methods are still limited to traditional in vitro ejaculation, sterilization and condom use, and there is no new efficient, convenient, safe and reversible contraceptive measure. [0003] For decades, researchers have tried to use reproductive-related hormones and sperm-specific proteins as target antigens to apply immunization methods to male birth control, but there are still many problems, such as not yet finding the best specific target antigens, and the lack of natural ant...

AI Technical Summary

Problems solved by technology

Male rhesus monkeys immunized with the Eppin truncated protein retaining the carboxy-terminal showed reversible infertility (contraceptive rate 7 / 9, reversible rate 5 / 7), semen analysis found that the forward movement ability of sperm was weakened, and the number of sperm and male sex in the body were weak. Hormone levels were not affected, and no autoimmune diseases occurred, indicating that Eppin is a promising target antigen, but it is a self-antigen, so there are still problems such as weak immunogenicity and immune tolerance. , did not eliminate or process the undesired peptides in Eppin that may cause long-term toxic and side effects

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