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Method for screening nitrification bacteria

The technology of a heterotrophic nitrifying bacteria and a screening method is applied in the field of sewage treatment, which can solve the problems of difficult treatment and slow growth of high-ammonia-nitrogen wastewater, and achieve the effect of improving effective treatment and good application prospects.

Inactive Publication Date: 2009-05-20
CHINA PETROLEUM & CHEM CORP +1
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for screening heterotrophic nitrifying bacteria, which is used in the biochemical treatment process of high ammonia nitrogen industrial wastewater to solve the problem of difficult treatment of high ammonia nitrogen wastewater caused by the slow growth of autotrophic nitrifying bacteria in the traditional wastewater biochemical treatment process

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  • Method for screening nitrification bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Nitrification sludge enrichment

[0026] The bottom of the reaction tank of the nitrifying bacteria enrichment device used is equipped with an aeration head and air-lift stirring; the effective volume of the reaction system is 2.2L, and the aeration is stopped every 24h for 30min, and 1.5L of new water is replaced; the activated sludge comes from Fushun Petroleum No. 3 Plant Wastewater treatment workshop aeration tank, initial SV30 is 30%; cultivation parameters: T (temperature): 25-30°C; pH: 6.5-8.5; dissolved oxygen DO>2.0mg / l; slowly add 90g / L NaHCO 3 The solution supplements the alkalinity consumed by the nitrifying bacteria to reduce the consumption of ammonia nitrogen. Ammonia nitrogen influent is artificially prepared enriched culture solution, and the NH in the enriched culture solution is gradually increased. 3 -N concentration, enriching nitrifying bacteria that can tolerate high concentrations of ammonia nitrogen. The enrichment medium used consis...

Embodiment 2

[0028] Example 2 Separation and Purification of Heterotrophic Nitrifying Bacteria from Activated Sludge Enriched in Nitrifying Bacteria

[0029] The culture medium (HNM) for separating and purifying heterotrophic nitrifying bacteria from the activated sludge enriched in nitrifying bacteria adopts a medium containing (NH 4 ) 2 SO 4 2.4g / L, glycerol 16g / L, nutrient salt solution 40ml (2% agar was added to the solid medium). Nutrient salt solution contains K per liter 2 HPO 4 6.4g, MgSO 4 ·7H 2 O 3g, NaCl 2.5g, Fe 7H 2 O 0.04g, MgSO 4 4H 2 O 0.05g, EDTA 4g. The operation process is as follows: Take 5ml of mud-water suspension from the activated sludge enriched in nitrifying bacteria, use an ultrasonic cell breaker to break up the flocculent activated sludge, let it stand for 30min, take 0.5ml of the upper liquid and inoculate it into 100ml of HNM culture medium (30°C, 200rpm) for 48 hours, during which 9% (w / v) sterilized NaHCO was used regularly 3 The pH of the cultu...

Embodiment 3

[0030] Example 3 Simultaneous nitrification and denitrification of high COD and high ammonia nitrogen wastewater by FN-4 bacteria

[0031] Inoculate FN-4 in C-containing HNM medium at 30°C, shake at 200rpm for 48h, collect cells by centrifugation, resuspend in 200ml artificial water containing 300mg / mL ammonia nitrogen, 24g / L methanol (to simulate high COD, high ammonia nitrogen wastewater ), 30°C, 200rpm shaking culture, every 24h centrifugation to collect the bacteria to replace fresh HNM medium, during which the alkali solution was used to adjust the alkalinity consumed by the degradation of ammonia nitrogen in the reaction system regularly. The concentration of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is detected in the effluent, and the results are shown in figure 2 . At the same time, a shake flask without bacteria was added to add artificial water to shake it as a control of the initial ammonia nitrogen concentration. A total of 16 comparisons were made...

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Abstract

The invention relates to a screening method of heterotrophic nitrifier. The screening method comprises the following steps: (1) nitrifier-enriched activated sludge which can tolerate high ammonia nitrogen loads is cultured by a method of gradually improving the ammonia nitrogen loads; (2) the heterotrophic nitrifier is separated and purified from the nitrifier-enriched activated sludge; (3) the nitrification of the heterotrophic nitrifier which is separated and purified is inspected; and (4) the species identification of the heterotrophic nitrifier which is separated and purified is carried out. The heterotrophic nitrifier which is screened by utilizing the method has rapid growth rate and can not only carry out synchronous nitrification and denitrification to high-COD and high ammonia nitrogen industrial waste water, but also can carry out high-efficient nitrification to the low-COD and high ammonia nitrogen waste water.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and in particular relates to a screening method for heterotrophic nitrifying bacteria. Background technique [0002] Biological denitrification of wastewater is a hot topic in the field of water treatment. It has long been believed that nitrifying bacteria are composed of some autotrophic bacteria. However, when the environmental conditions are not suitable for the growth of autotrophic nitrifying bacteria, some heterotrophic microorganisms with nitrifying activity will also grow. For example, as early as 1894, Stutzer and Hartleb described the phenomenon of heterotrophic nitrification of a fungus (Stutzer H, Hartleb R. Uber nitrabildung zentralblbakteriol parasitonkd infktionskr. Hyg Abt l Oring Reihe A, 1894, 22: 701). Since then, many reports have confirmed that the process of generating nitrate / nitrite from ammonium salts not only exists in autotrophic ammonia oxidizing bacteria (such as Nitr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12Q1/04
Inventor 张全佟明友高会杰黎元生唐似茵
Owner CHINA PETROLEUM & CHEM CORP
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