Method for rapidly extracting tyrosinase from potato
A tyrosinase and potato technology, applied in the direction of oxidoreductase and the like, can solve the problems of large experimental error, high cost, cumbersome process, etc., and achieve the effects of maintaining tyrosinase activity, fast operation and convenient source.
Inactive Publication Date: 2009-05-20
DONGHUA UNIV +1
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Abstract
The invention relates to a method for quickly purifying tyrosinase (TYR) from potatoes and comprises the following steps: (1) the potatoes are smashed into homogenate and salted out, precipitate is dissolved and dialyzed so as to extract a raw TYR enzyme; (2) a FPLC gel molecular sieve or anion exchanging chromatography are used for purifying the TYR; and (3) polyacrylamide gel electrophoresis and enzymatic activity measurement with an ultraviolet spectrophotometer are used for representing the TYR. The method is quick, simple and convenient, and has low cost and higher purified TYR activity.
Application Domain
Oxidoreductases
Technology Topic
ChemistryMolecular sieve +7
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