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Method for extracting oryzanol

An extraction method and technology of oryzanol, applied in the direction of steroids, organic chemistry, etc., can solve the problems of long production cycle, large loss of neutral oil, low yield of oryzanol, etc., and achieve the effect of increasing added value and reducing loss rate

Active Publication Date: 2009-05-27
JIANGNAN UNIV
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  • Abstract
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Problems solved by technology

[0003] Although oryzanol is obtained, this method still has certain defects: the production cycle is long, and it takes 10 days from soapstock to oryzanol semi-finished products; the yield of oryzanol is low, only about 15%
Although great progress has been made, the yield of oryzanol is only 30% to 35%, and due to the strong alkalinity of NaOH, both methods lead to saponification of a large amount of neutral oil, and the yield of refined rice bran oil is only 50% to 55%.
[0005] Afterwards, many other oryzanol production methods emerged: "three-in-one" refining method, double-solvent fractionation method, etc., all encountered the same problems as the first two methods: low yield of oryzanol, loss of neutral oil Big

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  • Method for extracting oryzanol
  • Method for extracting oryzanol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: The establishment of the ultraviolet spectrophotometry quantitative analysis method of oryzanol content

[0016] Determination of the standard curve: Accurately weigh 0.0219g of oryzanol standard substance in a 100mL beaker with an electronic balance, add 2mL of chloroform to dissolve it, and then dilute it in a 50mL volumetric flask with absolute ethanol (concentration 0.438 μg / mL), shake and shake well, as a standard solution, accurately measure 0.5, 1.0, 1.5, 2.0, 2.5mL of the solution in a 50mL brown volumetric flask, dilute to the mark with absolute ethanol and shake well. Use a 1cm quartz cuvette, use absolute ethanol as a blank, measure the absorbance at (327±1nm), draw the standard curve with the concentration C (μg / mL) as the abscissa, and the absorbance as the ordinate. Obtain standard curve regression equation as A=0.0327C—0.0045, R 2 = 0.9992.

[0017] Sample determination: Weigh 2-3g of oil sample in a 50mL volumetric flask, dissolve it with...

Embodiment 2

[0023] Embodiment 2: the selection of ethanolamine concentration

[0024] According to the ratio of rice bran oil (m):n-hexane (V)=2:1, weigh 50g of crude rice bran oil and 25mL of n-hexane, put them into a three-necked flask (with a reflux device), then add ethanolamine solutions of different concentrations, stir, The reaction temperature was 70°C, and the reaction time was 30 minutes. The influence of ethanolamine concentration on the reaction was investigated. The results are shown in Table 1.

[0025] Table 1 Effect of ethanolamine concentration on loss rate, retention and deacidification rate of oryzanol in oil

[0026]

[0027] As can be seen from Table 1, when the concentration of ethanolamine is 20%, the loss rate of oryzanol in the oil is the lowest, and the deacidification rate is also high at this time. While removing most of the fatty acids, oryzanol has been greatly retained, so Ethanolamine works best at a concentration of 20%.

Embodiment 3

[0028] Embodiment 3: the selection of pH value

[0029] After pre-deacidification according to the method in Example 2, centrifuge, and then use 2% NaOH solution to enrich oryzanol in the pre-deacidification clear oil at a temperature of 70° C. for 30 minutes. After the reaction, take the soapstock, dissolve it with methanol, and filter to remove the unsaponifiable matter, so as to obtain a methanol solution containing oryzanol sodium salt and fatty acid sodium salt. Adjust the pH of this solution with citric acid to find the optimum pH for the precipitation of oryzanol, and the evaluation standard is the final quality (g) of oryzanol. According to this method, the optimum pH is selected as 5.5-6.0.

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Abstract

The invention provides a method for extracting oryzanol, which belongs to the field of oryzanol preparation. The method comprises the following steps: crude rice bran oil is used as a raw material, the rice bran oil and normal hexane mixing oil are subjected to pre-deacidification by ethanol first, and then oryzanol is enriched to nigre by NaOH solution, and oryzanol sodium salt is extracted from the nigre by methanol, and finally by regulating the pH value by acid solution, the oryzanol contained in the oryzanol sodium salt is separated out; when ethanolamine deacidification is applied to the production method of the oryzanol, nearly 20 percent loss of the oryzanol caused by alkali refining in the prior method is avoided, and the oryzanol yield is improved from 30 to 35 percent in the prior method to 60 to 70 percent in the method of the invention; moreover, because ethanol can not saponify glyceride, the loss rate of the crude oil caused by deacidification is greatly reduced, from 15 to 25 percent in the prior method to 2 to 5 percent in the method of the invention. The nigre ethanolamine fatty acid generated in the method can be used for cosmetics manufacturing industry to further increase added values.

Description

technical field [0001] The invention relates to an extraction method of oryzanol, which belongs to the field of oryzanol preparation. Background technique [0002] Oryzanol is the commercial name of cycloartenyl ferulate as a medicine. It is a natural mixture composed of cycloartenol ferulate as the main body and part of sterol ferulate. The content of oryzanol in rice bran oil is about 1.8% to 2.5%. Oryzanol is mainly used in pharmaceuticals. In recent years, oryzanol has entered the field of cosmetics as a skin care raw material, and a small amount is also used as an antioxidant in food oils. In 1969, oryzanol was jointly researched and developed by the Grease Research Office of Shanghai Grain and Oil Industry Company, the Oil Research Office of Zhejiang Institute of Grain Science Research Institute, and the Analysis Laboratory of Shanghai Pharmaceutical Industry Research Institute. It was successfully trial-produced in 1971, and the production method of oryzanol was for...

Claims

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Application Information

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IPC IPC(8): C07J53/00
Inventor 金青哲刘贺单良刘元法宋志华黄健花王兴国
Owner JIANGNAN UNIV