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Technology for simultaneously preparing catalase and liver peptide by using raw material of animal liver

A catalase and animal liver technology, applied in the field of separation and extraction of biologically active substances, can solve problems such as environmental pollution, insufficient utilization of raw materials, a large amount of waste residue and waste water, etc., achieve broad application prospects and eradicate environmental pollution problems , to achieve the effect of zero emissions

Inactive Publication Date: 2009-06-03
江苏省江大绿康生物工程技术研究有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product of this process is single, the raw materials are not fully utilized, and a large amount of waste residue and waste water will be generated in the production process, causing very serious pollution to the environment
[0005] While separating and extracting catalase, it can co-produce other high value-added products, improve the utilization rate of raw materials, eliminate environmental pollution problems in the process of catalase processing and production, and realize zero discharge of waste residue and wastewater in the processing and production process. The process of making the processing and production of catalase carried out in an environmentally friendly manner has not yet been reported at home and abroad

Method used

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  • Technology for simultaneously preparing catalase and liver peptide by using raw material of animal liver
  • Technology for simultaneously preparing catalase and liver peptide by using raw material of animal liver

Examples

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Effect test

example 1

[0013] Take 10kg of pig liver, chop it up, add 40L of water to homogenate it, and keep it warm at 30°C for 12 hours to make it autolyze. After reaching the specified time, adjust the pH value to 6.5, and then vacuum filter to obtain 40 L of filtrate with peroxidase activity of 20,000 U / ml and 9.5 kg of filter residue. The filtrate was concentrated by ultrafiltration with a hollow fiber ultrafiltration membrane with a cut-off molecular weight of 40 kDa to obtain 15 L of ultrafiltration concentrate with a peroxidase activity of 50,000 U / ml and 25 L of ultrafiltration permeate. The ultrafiltration permeate was concentrated with a roll-type reverse osmosis membrane to obtain 1.2 L of reverse osmosis concentrate and 23.7 L of reverse osmosis permeate. Combine the reverse osmosis concentrate with the filter residue, adjust the pH value to the optimum pH of pepsin, hydrolyze at the optimum temperature of pepsin for 12 hours, then adjust the pH value to 6.8, centrifuge (4°C, 8000rpm, ...

example 2

[0015] Take 5 kg of duck liver, mince it, add 20 L of water to homogenate it, and keep it at 35°C for 8 hours to make it autolyze. After reaching the specified time, adjust the pH value to 6.5, and then use a refrigerated centrifuge to centrifuge at 10°C and 9000 rpm to obtain 20 L of supernatant with a peroxidase activity of 28,000 U / ml and 5 kg of residue. The supernatant was concentrated by ultrafiltration with a hollow fiber ultrafiltration membrane with a cut-off molecular weight of 20 kDa to obtain 11 L of ultrafiltration concentrate with a peroxidase activity of 50,000 U / ml and 9 L of ultrafiltration permeate. The ultrafiltration permeate was concentrated with a roll-type nanofiltration membrane (molecular weight cut-off: 150) to obtain 0.4 L of nanofiltration concentrate and 8.5 L of nanofiltration permeate. Combine the nanofiltration concentrate with the residue, adjust the pH value to the optimum pH of trypsin, hydrolyze it at the optimum temperature of trypsin for 1...

example 3

[0017] Take 50kg of grass carp liver, chop it up, add 200L of water to homogenate it, and keep it warm at 33°C for 10 hours to make it autolyze. After reaching the specified time, adjust the pH value to 6.5, then use a butterfly refrigerated centrifuge to centrifuge at 4°C and 9000rpm, and then filter the obtained supernatant with a microporous membrane with a pore size of 5 μm to obtain the peroxidase activity. 155L of 32,000U / ml filtrate and 45kg of residue. The filtrate was concentrated by ultrafiltration with a roll-type ultrafiltration membrane with a cut-off molecular weight of 35 kDa to obtain 98 L of ultrafiltration concentrate with a peroxidase activity of 50,000 U / ml and 56 L of ultrafiltration permeate. The ultrafiltration permeate was concentrated with a roll-type nanofiltration membrane with a cut-off molecular weight of 150 Da to obtain 2.5 L of nanofiltration concentrate and 53.7 L of nanofiltration permeate. Combine the nanofiltration concentrate with the filt...

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Abstract

The invention belongs to the technical field of separation and extraction of bioactive substances, and in particular relates to a technique for preparing catalase and liver peptide by using raw material of animal liver. The technique is suitable for processing and production of catalase by using the raw material of animal liver after autolysis processing, and provides a novel technique of jointly producing catalase and liver peptide. The technique has the advantages that catalase and liver peptide can be obtained at the same time; no waste water and waste slag are discharged in the whole processing and production process; and environment pollution problem can be radically solved in the prior catalase processing and production technique. After providing catalase to animal liver, the remaining ezyme of liver peptide hydrolysates the product, so as to be utilized as a novel natural drug raw material or a functional health food raw material and widely used in the fields of drugs, health-care products, food, etc. By adopting the invention for operation, taking pig liver as an example, 2 kg of catalase (50,000 U / ml) can be prepared from 1 kg of pig liver, at the same time, 200 g of liver peptide powder with protein content reaching 70% can be obtained, and the water consumption is reduced to lower than 2.2 kg.

Description

technical field [0001] The invention relates to a process for simultaneously preparing catalase and liver peptide by using animal liver as a raw material, and belongs to the technical field of separation and extraction of biologically active substances. Background technique [0002] Catalase is an oxygen-removing enzyme whose function is to efficiently decompose hydrogen peroxide into water and oxygen, and has a wide range of uses in textile, food and other industries. In the textile industry, catalase is applied to the biological deoxygenation and purification process of textiles after oxygen bleaching, which can greatly save water and energy, and remove the dye decomposition that may occur in subsequent dyeing of reactive dyes in fabrics and water baths after oxygen bleaching Or the residual hydrogen peroxide of dyeing defects, shorten the process time, improve the dyeing quality, and reduce the discharge of printing and dyeing wastewater. The enzyme is also suitable for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08C12P21/06
Inventor 汤鲁宏吴保承刘磊杨春霞邓超陈伟方鑫
Owner 江苏省江大绿康生物工程技术研究有限公司
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