Method for preparing melon tree extract
A papaya and extract technology, which is applied in the field of preparation of papaya extracts, can solve the problems that the deep processing of papaya has not been better developed, nutrient loss, and cannot be stored, and achieves less investment in production equipment, reduced loss, Easy to use effect
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Embodiment 1
[0026] Take 3150g of fresh and ripe papaya, peel and remove seeds, weigh 2450g, add 7.4L of water at a ratio of 1:3, mash with a mixer, heat to 42-45°C, and adjust the pH to 3.8 with 0.1mol / L HCl or sulfuric acid According to the dosage ratio of pectinase 750u / g papaya, add 1.84 million units of pectinase, stir well, and maintain pH3.5~4.0, 42℃~45℃ for 3 hours for hydrolysis. After enzymatic hydrolysis, heat up to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and lower the temperature to below 30°C. Suction filter to obtain 6.5L of supernatant, centrifuge the residue with a centrifuge, collect the supernatant to obtain 0.5L, combine the supernatant, a total of 7.0L, adjust the pH to 6.8-7.0 with 1mol / L NaOH, and set the molecular cutoff to 100K Ultrafiltration was carried out with a filter membrane, and the filtrate was collected to obtain 6.5 L, which was the refined solution of papaya extract. Sampling for detection of polysaccharides, peptides, content, acid...
Embodiment 2
[0028] Take 2150g fresh and ripe papaya, weigh 1677g after peeling and removing seeds, add 5.0L of water at a ratio of 1:3, mash with a mixer, heat to 42-45°C, and adjust the pH to 8.0-9.0, according to the dosage ratio of glucoamylase 150u / g papaya, add 250,000 units of glucoamylase, stir well, maintain pH 8.0, and hydrolyze at 42°C-45°C for 3 hours. After enzymatic hydrolysis, heat up to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and lower the temperature to below 30°C. Suction filter to obtain 4.4L supernatant, centrifuge the residue with a centrifuge, collect the supernatant to obtain 0.3L, combine the supernatant, a total of 4.7L, adjust the pH to 6.8-7.0 with 0.1mol / L HCl, and reserve the molecular cut-off of 100K Ultrafiltration was carried out with a filter membrane, and the filtrate was collected to obtain 4.4 L, which was the refined solution of papaya extract. Sampling for detection of polysaccharides, peptides, content, acidity, etc., for later u...
Embodiment 3
[0030] Take 4550g of fresh and ripe papaya, weigh 3549g after peeling and removing seeds, add 10.6L of water at a ratio of 1:3, mash with a mixer, heat to 42-45°C, and adjust the pH to 0.1mol / L citric acid or phosphoric acid. 4.5-5.5, according to the dosage ratio of 500u / g papaya, add 1.78 million units of cellulase, stir well, maintain pH 4.5, 42°C-45°C for 3 hours for hydrolysis. After enzymatic hydrolysis, heat up to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and lower the temperature to below 30°C. Suction filter to obtain 9.3L of supernatant, centrifuge the residue with a centrifuge, collect the supernatant to obtain 0.6L, combine the supernatant, a total of 9.9L, adjust the pH to 6.8-7.0 with 1mol / L NaOH, and set the molecular cutoff to 100K Ultrafiltration was performed on a filter membrane, and the filtrate was collected to obtain 9.3 L, which was the refined solution of papaya extract. Sampling for detection of polysaccharides, peptides, content, a...
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