Method for preparing 2,3,4,5,6-5-O-benzyl mannose from D-mannitol
A technology of mannitol and mannose, which is applied in 2 fields, can solve the problems of environmental pollution, mannose is not cheap, mercaptan odor, etc., and achieve the effect of reducing pollution, low price and mild reaction conditions
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[0007] The invention discloses a method for preparing 2,3,4,5,6-penta-O-benzylmannose from D-mannitol, which comprises the following steps:
[0008] Step 1: Prepare 1-trityl mannitol, dissolve D-mannitol in excess dry pyridine, add triphenylchloromethane, after the reaction is completed, extract, dry, and perform silica gel column chromatography to obtain 1-trityl Mannitol;
[0009] Step 2: Prepare 2,3,4,5,6-penta-O-benzyl-1-trityl mannitol, add 1-trityl mannitol and chlorobenzyl to NaH, dry DMF, react After steam distillation, extraction, washing, drying and silica gel column chromatography, 2,3,4,5,6-penta-O-benzyl-1-trityl mannitol was obtained;
[0010] Step 3: Preparation of 1,2,3,4,5-penta-O-benzylmannitol to CH 2 Cl 2 , MeOH dissolved 2,3,4,5,6-penta-O-benzyl-1-trityl mannitol, add BF 3 ·Et 2 O solution reaction, extraction, washing and drying to obtain 1,2,3,4,5-penta-O-benzylmannitol;
[0011] Step 4: Preparation of 2,3,4,5,6-penta-O-benzylmannose to CrO 3 , py...
Embodiment 1
[0020] Add 5.0g (27.5mmol) D-mannitol and 100mL anhydrous pyridine into a 250mL round bottom flask, stir and dissolve until the solution is clear, add 3.8g (13.6mmol) triphenyl in 10 times in 3 hours under ice bath methyl chloride. After 2 hours of reaction in ice bath, the temperature was raised to 80° C. for 14 hours. Concentrate to remove pyridine (toluene is co-evaporated), add 30mL water to dissolve the reaction mixture, and sequentially extract with 5×40mL dichloromethane (the combined aqueous phase is concentrated and dried to recover unreacted mannitol), and the organic phase Na 2 SO 4 Drying, filtration, concentration, and silica gel column chromatography (V (petroleum ether): V (ethyl acetate) = 4:1 → 2:1 → ethyl acetate elution) gave 3.2 g of colorless syrupy 1-triphenyl Methylmannitol (55% yield from triphenylchloromethane).
[0021] 4.8g (0.2mol) of NaH, 50mL of anhydrous DMF, heated at 50°C, and dropwise added 23mL (0.2mol) of BnCl to the system while stirring...
Embodiment 2
[0025] Add 5.0g (27.5mmol) D-mannitol and 100mL anhydrous pyridine into a 250mL round-bottomed flask, stir and dissolve until the solution is clear, add 2.5g (9.0mmol) triphenylchloromethane at room temperature, and react for 3d at room temperature . Concentrate to remove pyridine (toluene is co-evaporated), add 30mL water to dissolve the reaction mixture, and sequentially extract with 5×40mL dichloromethane (the combined aqueous phase is concentrated and dried to recover unreacted mannitol), and the organic phase Na 2 SO 4 Drying, filtration, concentration, and silica gel column chromatography (V (petroleum ether): V (ethyl acetate) = 4:1 → 2:1 → ethyl acetate elution) gave 2.3 g of colorless syrupy 1-triphenyl Methylmannitol (62% yield from triphenylchloromethane).
[0026] 6.0g (0.25mol) of NaH, 50mL of anhydrous DMF, heated at 65°C, and added dropwise 29mL (0.25mol) of BnCl to the system while stirring, and simultaneously added dropwise 9.7g (23.0mmol) of 1- Trityl mann...
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