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Method for extracting transfer factor from pig spleen

A technology of transfer factor and spleen, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of long production cycle, high operating costs, and difficulty in industrialized large-scale production, so as to save manpower and material resources, improve Production efficiency and the effect of shortening the production cycle

Active Publication Date: 2009-07-08
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, the existing transfer factor technology usually removes the fat and large blood vessels in the spleen of the animal, then smashes the tissue, freezes and thaws it repeatedly, and obtains it through semi-permeable membrane dialysis, which is expensive to operate
In addition, the Laurence method is routinely used, that is, the transfer factor is recovered by two dialysis methods. This method is simple to operate, but the production cycle is long and cumbersome (the production cycle takes about 20 days), and it takes a long time and wastes a lot of manpower and material resources.
Therefore, these methods are difficult to carry out large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of transfer factor

[0042] 1. Cutting and cleaning: Remove the fat and blood vessels from the animal spleen that has thawed into a semi-frozen state, accurately weigh 1 kg, and wash 3 times with non-pyrogenic distilled water.

[0043] 2. Grinding: Weigh the degreased spleen and grind it three times in a meat grinder. Add 500ml of pyrogen-free distilled water to the minced spleen, put it in a mashing tank, and finely grind it.

[0044] 3. Grinding: put the mashed spleen pulp into a colloid mill to grind it to make a homogenate.

[0045] 4. Cell disruption: Homogenize the above-mentioned spleen homogenate in a high-pressure homogenizer at a pressure of 60 MPa to make a homogenate.

[0046] 5. Add 240 ml of 1% chitosan solution to the homogenate that has been homogenized under high pressure, put it in a refrigerated centrifuge at 7000 rpm, centrifuge for 15 minutes, and centrifuge at a temperature of 4° C., discard the sediment, and collect ...

Embodiment 2

[0048] Embodiment 2: detection of transfer factor prepared by process of the present invention

[0049] The transfer factor stoste prepared by the process described in Example 1 is prepared according to the State Drug Administration, the National Drug Standard WS 1 -X6-036-2000 performs the following detections:

[0050] 1. The appearance is a light yellow clear liquid, in line with the appearance of transfer factor solution.

[0051] 2. Amino acid identification reaction is a positive reaction.

[0052] 3. Ultraviolet spectrophotometric measurement: the sample obtained above has an absorption peak at 251±2nm, and ABS 250 / ABS 280 >2.0.

[0053] 4. The pH value is between 6.0 and 7.5.

[0054] 5. Detection of 20% sulfosalicylic acid: the sample obtained above has no turbidity and precipitation, indicating that the protein reaction is negative, and it does not contain macromolecular protein.

[0055] 6. In the detection of high molecular weight substances, there is no abs...

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Abstract

The invention provides a method for acquiring a transfer factor from spleens of animals, which comprises the steps of tissue smashing, cell disruption by a high pressure homogenizer, the precipitation by a flocculating agent, the filtration by a microporous filtering film, the ultrafiltration by an ultrafiltration membrane and the like, wherein the flocculating agent is added during the wall breaking, which ensures that protein in a solution is precipitated and the solution is clarified, and reduces the possibility of blocking a hollow fiber ultrafiltration column during the ultrafiltration. The method can greatly shorten production period, save a large quantity of manpower and physical resources, improve the production efficiency of the transfer factor, and provide a feasible approach for satisfying the industrialized mass production.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, in particular to a method for extracting transfer factor from animal spleen. The content of the invention mainly relates to a method for extracting transfer factor from pig spleen. Background technique [0002] Transfer factor (TF) is a substance released by T lymphocytes that can transfer sensitization information. It can specifically transfer the donor's cellular immune information to the recipient, thereby enhancing the immune function of the recipient. Since TF was discovered in the 1950s, scholars at home and abroad have conducted a lot of research on it, and introduced it into veterinary immunity and clinical treatment in 1979. At present, it has become a widely used immune-enhancing agent abroad. [0003] Since transfer factor has no species specificity, clinically there are many sources of transfer factor, such as human transfer factor, porcine transfer factor, bovine transfer factor, ...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K1/30
Inventor 夏雪林苏建东李旭东
Owner TIANJIN RINGPU BIO TECH
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