Multichannel photobacteria on-line water quality toxicity monitoring device and monitoring method

A technology of luminescent bacteria and monitoring devices, which is applied in the field of environmental monitoring, can solve the problems of not being able to use multiple luminescent bacteria at the same time, and achieve the effects of compact structure, convenient maintenance and simple operation

Inactive Publication Date: 2009-07-08
YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the existing equipment does not fully consider the diversity of this ba...

Method used

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  • Multichannel photobacteria on-line water quality toxicity monitoring device and monitoring method
  • Multichannel photobacteria on-line water quality toxicity monitoring device and monitoring method
  • Multichannel photobacteria on-line water quality toxicity monitoring device and monitoring method

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Cold storage and online recovery of luminescent bacteria

[0035] One of the core issues of the online monitoring device for water quality toxicity is to keep the biological activity of luminescent bacteria stable during the online operation, so as to ensure the reliability of continuous monitoring of the device. In the device system, the low-temperature storage tank of the detection component can quickly refrigerate highly active luminescent bacteria. Since the metabolism of luminescent bacteria is slow in the refrigerated environment of 5°C, once it is transferred to the environment of 20°C and revived, it can return to normal physiological activity in a short period of time. The formulation of the refrigerated reagent is as follows: medium (mass concentration) of Photobacterium brightens: 0.5% tryptone, 0.5% yeast extract, 0.3% glycerol, KH 2 PO 4 0.1%, Na 2 HPO 4 0.5%, NaCl 3%. Resuscitation solution (mass concentration): NaCl 2%. Using the above li...

Embodiment 2

[0036] Example 2: Pretreatment method of polluted water body by luminescent bacteria online detection system

[0037] The purpose of the pretreatment of the water toxicity online monitoring device is to ensure the long-term trouble-free operation of the main analysis flow path and the accuracy and reliability of the analysis results. Therefore, the suspended solids, sediment, and bacteria in the water must be removed, and at the same time, the comprehensive toxicity of the water must not be affected. . The development and design of the device system is attached image 3 The hollow fiber membrane online filter shown has an average pore diameter of 0.1 μm, and the membrane module consists of 70 membrane filaments, each of which is 300 mm long and 2 mm in diameter.

[0038] The pretreatment method is to use a diaphragm pump with a suction distance of 5m to pump the water sample into the filter, then pass through the filter membrane of the membrane module, and then lead it into t...

Embodiment 3

[0040] Example 3: luminescent bacteria on-line detection system detects Zn, cresol, ... pollutants

[0041] Using an online water quality toxicity monitoring device, the typical process of pollutant toxicity detection mainly includes six steps: adding buffer, adding bacteria, cultivating bacteria, adding water samples, inhibiting the reaction, and cleaning. The detection cycle of a sample is about 40min~60min Left and right, can be repeated automatically. The realization method of toxicity detection: taking cresol, a typical pollutant, as an example, the system sequentially adds a certain proportion of buffer solution and bacterial solution into the reactor and cultivates it for 10 minutes. Water samples, until the luminous intensity reaches the steady state of the lowest value, the data processing software will calculate the corresponding inhibition rate and EC after obtaining the highest and lowest values 50 value is fed back to the system. as attached Figure 5 Shown are...

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Abstract

The invention discloses a multi-channel photobacteria on-line water quality toxicity monitoring device and a monitoring method thereof. The device comprises a diaphragm water pump, wherein a water inlet of the diaphragm water pump is connected with a membrane filter through a water pipe and then is connected with a water sample pool; a water outlet of the diaphragm water pump is connected with an overflow type branch sampler through a water pipe; a plurality of water sample monitoring modules form a plurality of monitoring channels which are connected to the overflow type branch sampler; waste liquid outlets of the plurality of the water sample monitoring modules are parallelly connected with an external peristaltic pump; and an outlet of the peristaltic pump is connected to a waste liquid tank. The monitoring method comprises the following steps: a water sample is sucked into the membrane filter consisting of a hollow fibrous membrane by the diaphragm pump, suspensions and bacteria in water are filtered, and the water sample enters the overflow type branch sampler. Then the bioluminescent intensity of photobacteria is detected in a real-time mode through a toxicity detection module. After a detection experiment is finished, the external peristaltic pump discharges a waste liquid in a reactor into the waste liquid tank; and the device can realize the on-line detection of biologic toxicity of the water from water sources, municipal sewage, and input water of water works, and has the characteristics of simple operation, convenient maintenance, high automation degree, and so on.

Description

technical field [0001] The present invention relates to a monitoring device and a monitoring method capable of rapidly on-line detection of water quality toxicity. The early warning of toxic pollution accidents belongs to the field of environmental monitoring. Background technique [0002] In recent years, the types of toxic substances have gradually increased, and the total amount of emissions has continued to increase, and the resulting environmental hazards have gradually increased. In order to strengthen the management of river water and drinking water protection, industrial wastewater discharge, etc., relevant regulations have been issued internationally, requiring the realization of water quality toxicity control from 2007 to 2010. In order to ensure the safety of water quality in water sources, my country will also incorporate water toxicity into the control standards in recent years. At present, biotoxicity test methods mainly include three levels: molecular level,...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N1/34C12R1/01
CPCG01N33/1826Y02A20/20
Inventor 蔡强张理兵刘锐彭强辉曾莎莎
Owner YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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