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Transfer factor for dog, preparation method and application thereof

A transfer factor and solution technology, applied in the field of bioengineering, can solve the problems of low content of transfer factor in dialysate, difficulty in industrialized large-scale production, waste of manpower and material resources, etc., and achieve the goals of prolonging dialysis time, shortening production cycle, and saving manpower and material resources Effect

Inactive Publication Date: 2009-07-29
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that due to the restriction of dialysate corruption factors, the dialysis time is short, and the transfer factor content in the dialysate is low, only 0.4 to 0.5 mg per milliliter
[0005] In addition, the Laurence method is routinely used, that is, the transfer factor is recovered by two dialysis methods. This method is simple to operate, but the production cycle is long and cumbersome (the production cycle takes about 20 days), and it takes a long time and wastes a lot of manpower and material resources.
Therefore, these methods are difficult to carry out large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the preparation of transfer factor

[0041] (1) Remove fat, palpation and large blood vessels from 1 kg of frozen chicken spleen, wash with depyrogenated distilled water, cut into small pieces, and add 1 L of sterile saline;

[0042] (2) Carry out cell disruption with high-pressure homogenizer (100Mpa), add 1% chitosan solution of 240ml simultaneously as flocculant, make homogenate after mixing;

[0043](3) Freezing at -20°C and thawing at room temperature, repeated freezing and thawing 8 times;

[0044] (4) Then at 4°C, centrifuge at 6000rpm and take the supernatant. After repeating 3 times, put the supernatant in a dialysis bag and put the dialysis bag in a container filled with sterile saline, and place it in 0- Dialysis at 4°C for 72 hours;

[0045] (5) with 4% MaHCO 3 Adjust the pH value of the dialysate to neutral, and add 0.3% chloroform as a preservative;

[0046] (6) Filter the dialysate through a 0.45 μm microporous membrane, and then perform...

Embodiment 2

[0048] Embodiment 2: detection of transfer factor prepared by process of the present invention

[0049] The transfer factor stoste prepared by the process described in Example 1 is prepared according to the State Drug Administration, the National Drug Standard WS 1 -XG-036-2000 performs the following tests:

[0050] 1. The appearance is a light yellow clear liquid, in line with the appearance of transfer factor solution.

[0051] 2. Amino acid identification reaction is a positive reaction.

[0052] 3. Ultraviolet spectrophotometric measurement: the sample obtained above has an absorption peak at 251±2nm, and ABS 250 / ABS 280 >2.0.

[0053] 4. The pH value is between 6.0 and 7.5.

[0054] 5. Detection of 20% sulfosalicylic acid: the sample obtained above has no turbidity and precipitation, indicating that the protein reaction is negative, and it does not contain macromolecular protein.

[0055] 6. In the detection of high molecular weight substances, there is no absorpti...

Embodiment 3

[0064] Embodiment 3: the application of this transfer factor pair on dogs

[0065] For the transfer factor prepared by the process described in Example 1, the following research is carried out according to the requirements of the clinical trial research data of veterinary biological products issued by the Ministry of Agriculture Announcement No. 442:

[0066] 1. Experimental animals: 6 45-day-old healthy puppies, all of which were weaned and fed in a conventional way.

[0067] 2. Attacking the virus: attacking the same amount of canine parvovirus attenuated virus on 6 puppies, all of which caused the disease.

[0068] 3. Test method: When the symptoms appear, conduct a clinical treatment test immediately. Randomly divided into 3 groups, 2 in each group. The dose of intramuscular injection of transfer factor solution in the low dose group was 0.3ml / kg; the dose of intramuscular injection of transfer factor solution in the middle dose group was 0.6ml / kg; the dose of intramuscu...

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PUM

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Abstract

A preparation method of transfer factor solution applied on pets includes the following steps: frozen chicken spleen is washed with distilled water and then is cut into small blocks; pyrogen-free distilled water is added; cells are crushed with a high pressure homogenizer so as to be prepared into homogenate; pyrogen-free distilled water is added, blending is carried out and freeze thawing is repeatedly carried out for 8 times at the temperature of -20 DEG. C; the homogenate after freeze thawing is centrifugated so as to remove precipitates, the supernatant fluid is filtered with a microporous filtering film, and stock solution is obtained after hyperfiltration with an ultrafiltration membrane; 1 percent to 2 percent of zinc stearate is added as a stabilizing agent after virus is removed, and aseptic filtration is carried out through the microporous filtering film; and glycerin is added as a heat-resistant protecting agent after filtering, and direct packaging is carried out. The transfer factor prepared by the method can be used for sickness prevention and treatment of the pet dogs, thereby saving manpower and materials and increasing the extraction yield.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and belongs to a transfer factor used for improving dog immunity, and in particular relates to a transfer factor solution applied to dogs, a preparation method thereof and an application to dogs. Background technique [0002] As the pace of people's life and work continues to accelerate, the communication between people becomes less and less, so pets have become people's ideal spiritual sustenance, and with the continuous improvement of the status of pets in people's minds, some pet welfare It is also improving, so pet diseases are an issue that we must pay more attention to urgently. At present, some viral diseases in dogs have become a major problem for people. The existing general solution is to vaccinate. However, due to various reasons, sometimes it will inevitably cause immune failure, outbreak of disease, and bring certain difficulties to diagnosis and treatment. After immune fail...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K2/00A61K38/02A61P31/04A61P31/12A61P37/04
Inventor 苏建东王小娥李旭东
Owner TIANJIN RINGPU BIO TECH