Expression production and separation purification of recombinant placenta growth factor and chemical marker thereof

A placental growth factor, biotin labeling technology, applied in the field of biotechnology-recombinant genetic engineering, can solve the problems of low expression, high price and high cost of culture medium
CN101497883AActive Publication Date: 2009-08-05ACROIMMUNE BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ACROIMMUNE BIOTECH CO LTD
Publication Date
2009-08-05

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Abstract

The invention belongs to the field of biological technique, i.e. gene recombination engineering. Placental growth factors (PLGFs) play important roles in neovascularization and vascular proliferation; however, natural PLGF content in vivo is low and the natural PLGFs have short half-times, thus to extract sufficient entogenous PLGF proteins so as to satisfy the increasingly demands of experimental study and clinical practice is difficult. The invention discloses a method for efficiently expressing, producing and recombining a PLGF system in vitro by gene recombination engineering and cell culture technology and for extracting and purifying the PLGF proteins from expressed supernatant by chromatographic separating technology. The invention also discloses a nonradioactive chemical coupling labeling treatment method for the purified PLGF proteins in vitro. The PLGF proteins (including the unlabeled or the chemical coupling labeled) have wide application in the experimental study and the clinical practice.
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Description

technical field

[0001] The invention belongs to the field of biotechnology-recombinant genetic engineering, and relates to the expression and production of biologically active recombinant placental growth factor (placental growth factor, PLGF) by genetic engineering and eukaryotic cell fermentation and culture technology. The invention also relates to the separation and purification of the recombinant PLGF protein, its chemical labeling in vitro and its application. Background technique:

[0002] Angiogenesis or angiogenesis refers to the process in which blood vessels (such as capillaries and venules) in the body produce new blood vessels by sprouting or dividing. Angiogenesis is beneficial and necessary to maintain many normal physiological processes of the body, such as tissue embryonic development, wound healing and repair. However, excessive angiogenesis or hyperplasia is also closely related to many pathological changes (such as tumor proliferation, inflammatory respo...

Claims

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