Nano micromolecule hyaluronic acid and preparation method thereof

A technology of hyaluronic acid and small molecules, which can be used in food preparation, medical preparations containing active ingredients, and pharmaceutical formulas, etc. Control and other problems to achieve the effect of promoting wound healing, high skin affinity and permeability, and treating rough skin

Active Publication Date: 2009-08-19
BEIJING INST OF OLIGO PEPTIDE BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many ways to degrade hyaluronic acid, such as physical methods, which can degrade hyaluronic acid through heating, mechanical shear force, ultraviolet rays, ultrasonic waves, cobalt irradiation and other factors, but the degradation is limited, and it is difficult to obtain small nano-molecules. Hyaluronic acid; chemical degradation methods, including acid hydrolysis, alkali hydrolysis, sodium hypochlorite oxidative degradation, etc. The chemical degradation process is difficult to control, and browning and organic solvent residues are prone to occur

Method used

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  • Nano micromolecule hyaluronic acid and preparation method thereof
  • Nano micromolecule hyaluronic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Add 1000 g of water to 10 g of sodium hyaluronate (47.3 percent glucuronic acid content) with a molecular weight of 1.5 million, and stir to dissolve. Hydrolysis under acidic conditions: pH value is 2.5, hydrolysis at 85°C for 3 hours; enzymatic degradation: cool down to 50°C, add hyaluronidase (enzyme dosage 150,000 U / L), pH value is 5, hydrolysis at 50°C for 3 hours hours, and then heated to 90°C for 10 minutes to inactivate the enzyme. Filter with filter paper to obtain a transparent, colorless, odorless, transparent small molecule hyaluronic acid degradation solution. The above products are concentrated in vacuum at 60°C and spray-dried to obtain white powder small molecule hyaluronic acid.

[0017] After chemical analysis of the above liquid product, the content of glucuronic acid, the disaccharide labeling unit of hyaluronic acid in the product molecule, reached 46.1%, and its effective retention rate after degradation reached 97.4%; the above liquid p...

Embodiment 2

[0020] Example 2: 50 g of sodium hyaluronate (47.3% glucuronic acid content) with a molecular weight of 1.5 million was added to 950 g of water and stirred to dissolve. Hydrolysis under acidic conditions: pH value is 2, hydrolysis at 70°C for 4 hours; enzymatic degradation: cool down to 40°C, add hyaluronidase (enzyme dosage: 800,000 U / L), pH value is 6, hydrolysis at 40°C for 3 hours hours, and then heated to 90°C for 10 minutes to inactivate the enzyme. Filter with filter paper to obtain a transparent, colorless, odorless, transparent small molecule hyaluronic acid degradation solution. The above products are concentrated in vacuum at 60°C and spray-dried to obtain white powder small molecule hyaluronic acid.

[0021] After chemical analysis of the above liquid product, the content of glucuronic acid, the disaccharide labeling unit of hyaluronic acid in the product molecule, reached 46.5%, and its effective retention rate after degradation reached 98.3%; the above liquid pr...

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Abstract

The invention relates to a method for preparing nanometer low molecular hyaluronic acid, which comprises the following steps that: ultralow molecular weight hyaluronic acid with an average molecular weight less than 1,000D, namely nanometer hyaluronic acid oligomer, is obtained from hyaluronic acid with a molecular weight from tens of thousands to millions through hydrolysis methods such as enzyme and the like. The nanometer low molecular hyaluronic acid obtained by the method has functional characteristics of promoting the formation of capillary vessels, promoting the healing of wounds, having remarkable skin affinity and permeability and the like which are remarkably different from common hyaluronic acid, and can be taken as a high-grade raw material for cosmetology, health care food, and medicine.

Description

technical field [0001] The present invention relates to a method for preparing nanometer small molecular hyaluronic acid, specifically, hydrolyzing large molecular weight hyaluronic acid through enzymes and other methods to obtain ultra-low molecular weight hyaluronic acid with an average molecular weight of less than 1000 Daltons, namely nanometer hyaluronic acid. Grade oligomeric hyaluronic acid (NOHA). The nanometer small molecular hyaluronic acid obtained by this method has functional characteristics such as promoting angiogenesis, promoting wound healing, remarkable skin affinity and permeability, which are significantly different from ordinary hyaluronic acid, and can be used as a good medicine, health care product, Cosmetic raw materials. Background technique [0002] Hyaluronic acid (HA) is a mucopolysaccharide that exists in organisms, especially subcutaneous tissue. Due to its high moisturizing function, it is widely used as a raw material for cosmetics. In addit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/728A61K8/73A61P17/02A61Q19/00A23L1/30A23L33/125
Inventor 杜以波胡如桂杜铁柱
Owner BEIJING INST OF OLIGO PEPTIDE BIOLOGY
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