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Polysaccharide with antitumor and immunologic adjuvant function as well as preparation method and purpose thereof

An immune adjuvant and anti-tumor technology, applied in anti-tumor drugs, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve problems such as patient damage, achieve low toxicity, good safety, and improve spleen cell proliferation response. Effect

Inactive Publication Date: 2012-06-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past, people put more emphasis on eradicating tumors, but in practice, cancer cells are often killed, and the patient is dying. The damage caused to the patient is irreversible.

Method used

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  • Polysaccharide with antitumor and immunologic adjuvant function as well as preparation method and purpose thereof
  • Polysaccharide with antitumor and immunologic adjuvant function as well as preparation method and purpose thereof
  • Polysaccharide with antitumor and immunologic adjuvant function as well as preparation method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of polysaccharides of white vine pear

[0042] Preparation of total polysaccharides of Baiteng pear

[0043] The medicinal material Baiteng pear coarse powder 5kg was extracted by heating and refluxing with water for 2 hours, filtered, and the dregs were continuously extracted twice by refluxing and refluxing water. Combine the filtrates, recover under reduced pressure to 2000ml, add 3 times the volume of 95% ethanol with a mass concentration, mix well, and let stand at 4°C for 24 hours. The precipitate was collected and washed three times with ethanol, acetone and petroleum ether successively. The precipitate was dissolved in distilled water, dialyzed, and the retentate was concentrated under reduced pressure and freeze-dried to obtain the crude polysaccharide of Baiteng pear. The white vine pear crude polysaccharide is dissolved in 0.1mol / L sodium chloride, filtered through a 0.45 μm microporous membrane, purified on a DEAE-Sephadex A-5...

Embodiment 2

[0052] Embodiment 2: Anti-tumor effect in vivo of total polysaccharides of Baiteng pear

[0053] Take 50 clean-grade ICR mice, and subcutaneously inoculate each mouse with mouse sarcoma S 180Cell suspension 0.2ml. 24 hours after inoculation, they were weighed and randomly divided into groups of 10 rats. They were set as tumor-bearing control group, positive control group of cyclophosphamide (CTX), low, medium and high dose test groups of total polysaccharides of pear pear (AEPS). The tumor-bearing control group was orally administered normal saline at 0.2ml / 10g per day for 10 consecutive days; the positive control group was intraperitoneally injected with CTX at a dose of 20mg / kg, once every other day, a total of 5 times; Dosage groups were intragastrically administered at doses of 2.5 mg / kg, 5 mg / kg and 10 mg / kg, once a day for 10 consecutive times. The animals were sacrificed on the 11th day, the tumor tissues were taken out, weighed, and the tumor inhibition rate was cal...

Embodiment 3

[0058] Example 3: Effects of Baiteng pear total polysaccharides on the immune function of tumor-bearing mice

[0059] Take 60 clean-grade ICR mice, except for 10 mice in the normal control group, each mouse was subcutaneously inoculated with mouse sarcoma S in the right armpit. 180 Cell suspension 0.2ml. 24 hours after inoculation, they were weighed and randomly divided into groups of 10 rats. They were set as normal control group, tumor-bearing control group, cyclophosphamide (CTX) positive control group, and low, medium and high dose test groups of total polysaccharides of pear pears (AEPS). The normal control group and the tumor-bearing control group were orally administered normal saline at 0.2ml / 10g per day for 10 consecutive days; the positive control group was intraperitoneally injected with CTX at a dose of 20mg / kg, once every other day, a total of 5 times; , medium and high dose groups were intragastrically administered at doses of 2.5 mg / kg, 5 mg / kg and 10 mg / kg, o...

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Abstract

The invention relates to polysaccharide with antitumor and immunologic adjuvant function, a preparation method thereof, a medicine composite using the same as an active component, and an application oThe invention relates to polysaccharide with antitumor and immunologic adjuvant function, a preparation method thereof, a medicine composite using the same as an active component, and an application of the same in tumor treatment and adjuvant used as vaccine. The polysaccharide is kiwifruit polysaccharide A, kiwifruit polysaccharide B, kiwifruit polysaccharide C, kiwifruit polysaccharide D, and kif the same in tumor treatment and adjuvant used as vaccine. The polysaccharide is kiwifruit polysaccharide A, kiwifruit polysaccharide B, kiwifruit polysaccharide C, kiwifruit polysaccharide D, and kiwifruit total polysaccharide as a mixture containing the four kiwifruit polysaccharide, which are all extracted and separated from the Chinese medicinal herb of kiwifruit roots, and the polysaccharidewifruit total polysaccharide as a mixture containing the four kiwifruit polysaccharide, which are all extracted and separated from the Chinese medicinal herb of kiwifruit roots, and the polysaccharide has the remarkable antitumor and immunologic adjuvant function. The polysaccharides can obviously inhibit the growth of transplantation S180 caruncle and H22 liver cancer of a mouse, improve the breehas the remarkable antitumor and immunologic adjuvant function. The polysaccharides can obviously inhibit the growth of transplantation S180 caruncle and H22 liver cancer of a mouse, improve the breeder reaction of splenic cells of a tumor-bearing mice, the activity of NK cells and CTL, the ability of the splenic cells secreting IL-2 and IFN-Gamma, the level of tumor antigen specific antibodies Ider reaction of splenic cells of a tumor-bearing mice, the activity of NK cells and CTL, the ability of the splenic cells secreting IL-2 and IFN-Gamma, the level of tumor antigen specific antibodies IgG, IgG2a and IgG2b in blood serum of the tumor-bearing mouse, strengthen the humoral immunity and cell-mediated immunity response of an egg-albumin immunized mouse, induce the organism to produce Th1gG, IgG2a and IgG2b in blood serum of the tumor-bearing mouse, strengthen the humoral immunity and cell-mediated immunity response of an egg-albumin immunized mouse, induce the organism to produce Th1type and Th2 type immunity responses, and can be used as immunologic adjuvant of antineoplastic and tumor vaccine with immunity adjustment. type and Th2 type immunity responses, and can be used as immunologic adjuvant of antineoplastic and tumor vaccine with immunity adjustment.

Description

technical field [0001] The present invention relates to Baitengli polysaccharide A, Baitengli polysaccharide B, Baitengli polysaccharide C and Baitengli polysaccharide D which are extracted and separated from the Chinese herbal medicine Baitengli and have anti-tumor and immune adjuvant effects, and Baitengli polysaccharide containing these four polysaccharides Polysaccharides, their preparation methods, pharmaceutical compositions with the polysaccharides as active ingredients, and their applications in treating tumors and as vaccine adjuvants. Background technique [0002] Tumor is one of the most difficult diseases in the world today, and it is the second leading cause of death after cardiovascular disease, which seriously threatens people's life and health and brings huge pressure to society and families. The global antineoplastic drugs account for about 4.2% of the total sales of the drug market. Although the share is not high, the annual growth rate has remained at doub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61K39/39A61P35/00C08B37/00A61K31/715
Inventor 孙红祥吴远文
Owner ZHEJIANG UNIV
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