Preparation method of polypeptide-chitosan composite nanoparticles loaded with coenzyme Q10

A technology of composite nanoparticles and chitosan, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of singleness, easy aggregation, poor stability, etc., and avoid aggregation phenomenon, good biocompatibility and high stability

Inactive Publication Date: 2011-06-29
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to propose a polypeptide-chitosan composite loaded with coenzyme Q10 in order to overcome the shortcomings of the prior art that the drug carrier material stays in a single drug-loading system, and the single drug-loading system has poor stability and easy aggregation. Nanoparticles and methods for their preparation

Method used

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  • Preparation method of polypeptide-chitosan composite nanoparticles loaded with coenzyme Q10
  • Preparation method of polypeptide-chitosan composite nanoparticles loaded with coenzyme Q10
  • Preparation method of polypeptide-chitosan composite nanoparticles loaded with coenzyme Q10

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Dissolve 2.5 mg of PBLG-b-PEG block copolymer and 3 mg of coenzyme Q10 mixture in 25 mL of dimethylformamide (DMF) / tetrahydrofuran (THF) mixed solvent at room temperature, put it into a dialysis bag after completely dissolving, and Dialyze in deionized water for 72 hours, and replace with fresh deionized water every 3 hours to remove organic solvents. After dialysis in deionized water, the unembedded drug was removed by centrifugation, and the supernatant was filtered with a filter membrane with a diameter of 0.45 μm to obtain a drug-loaded micellar solution.

[0033] Measure 20mL of the coenzyme Q10-polypeptide self-assembly nano solution that has been prepared, add acetic acid to make the concentration of acetic acid 1.5mg / mL, and dissolve 15mg of chitosan in 10mL of acetic acid solution of the same concentration, and wait for chitosan After the sugar is completely dissolved, mix and stir the acetic acid solution of coenzyme Q10-polypeptide and chitosan, and add 30 mL...

Embodiment 2

[0035] Dissolve 2.5 mg of PBLG-b-PEG block copolymer and 8 mg of coenzyme Q10 mixture in 25 mL of dimethylformamide (DMF) / tetrahydrofuran (THF) mixed solvent at room temperature. Dialyze in deionized water for 72 hours, and replace with fresh deionized water every 3 hours to remove organic solvents. After dialysis in deionized water, the unembedded drug was removed by centrifugation, and the supernatant was filtered with a filter membrane with a diameter of 0.45 μm to obtain a drug-loaded micellar solution.

[0036]Measure 20mL of the coenzyme Q10-polypeptide self-assembled nano solution that has been prepared, add acetic acid to make the concentration of acetic acid 1.5mg / mL, and dissolve 30mg of chitosan in 10mL of acetic acid solution of the same concentration. After the sugar is completely dissolved, mix and stir the acetic acid solution of coenzyme Q10-polypeptide and chitosan, and add 30 mL of sodium tripolyphosphate (TPP) solution with a concentration of 1.5 mg / mL dropw...

Embodiment 3

[0038] Dissolve 2.5 mg of PBLG-b-PEG block copolymer and 2.5 mg of coenzyme Q10 mixture in 25 mL of dimethylformamide (DMF) / tetrahydrofuran (THF) mixed solvent at room temperature, put it into a dialysis bag after completely dissolving, Dialyze in deionized water for 72 hours, and replace with fresh deionized water every 3 hours to remove organic solvents. After dialysis in deionized water, the unembedded drug was removed by centrifugation, and the supernatant was filtered with a filter membrane with a diameter of 0.45 μm to obtain a drug-loaded micellar solution.

[0039] Measure 20mL of the coenzyme Q10-polypeptide self-assembled nano solution that has been prepared, add acetic acid to make the concentration of acetic acid 1.5mg / mL, and dissolve 30mg of chitosan in 10mL of acetic acid solution of the same concentration. After the sugar is completely dissolved, mix and stir the acetic acid solution of coenzyme Q10-polypeptide and chitosan, and add 30 mL of sodium tripolyphosp...

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Abstract

The invention relates to coenzyme Q10 loaded polypeptide-chitosan composite nanoparticles and a preparation method thereof. The method comprises that: poly-L-glutamate-gamma-benzyl-polyethyleneglycol segmented copolymer and the coenzyme Q10 are mixed at room temperature, and are dissolved in mixed solvent of dimethyl formamide and tetrahydrofuran; after dialysis, centrifugalization and the like, supernatant liquor is filtered to obtain a coenzyme Q10-polypeptide drug-loaded micelle solution, and acetic acid is added to regulate pH value; meanwhile, chitosan is dissolved in the acetic acid solution with the same pH value, and is mixed with the coenzyme Q10-polypeptide acetic acid, and trimeric sodium phosphate solution is dripped in the mixture, thereby obtaining the coenzyme Q10 loaded polypeptide-chitosan composite nanoparticles with particle diameter of between 80 and 900nm, average drug-loaded ratio of between 2 to 20 percent, and envelop rate of between 30 and 70 percent. The nanoparticles are coenzyme Q10 nanometer hydrophobic drug particles with higher stability, slow release, and good biocompatibility.

Description

technical field [0001] The invention relates to a nano drug-loaded particle for preparing fat-soluble drugs, in particular to a preparation method of a polypeptide-chitosan composite nano particle loaded with coenzyme Q10. Background technique [0002] Coenzyme Q10, also known as ubiquinone, is a natural antioxidant produced by the cells itself. It is safe and non-toxic, and can improve the immunity of the organism. Coenzyme Q10 is used as an anti-oxidant and activator of human skin, which has substantial effects on aged skin, and is an excellent anti-aging substance that can be used as both a drug and a health care product. However, the water solubility and photostability of coenzyme Q10 are poor, which affects its application. Select a suitable polymer carrier to embed coenzyme Q10 and prepare it into nanoparticles, which are used for drug delivery and controlled release. , can directly act on cells, increase drug stability, and prolong the characteristics of drug efficac...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P39/06A61P17/18A61K9/14A61K47/48A61P37/04A61K47/34A61K47/36
Inventor 章苏宁张健贺振颖
Owner SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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