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Method for preparing high content soybean oligosaccharide by simulated moving bed separation

A technology of soybean oligosaccharide and simulated moving bed, which is applied in separation methods, preparation of oligosaccharides and sugar derivatives, etc., can solve the problems of high separation cost, low separation strength, large amount of stationary phase and mobile phase, etc. The effect of short process, strong operability and mature process conditions

Inactive Publication Date: 2011-12-21
南京凯通粮食生化研究设计有限公司
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Problems solved by technology

This kind of treatment method is a batch operation, the amount of stationary phase and mobile phase is large, the separation strength is low, the concentration of the separated product is low, and the separation cost is high, so it is not suitable for industrial production.

Method used

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  • Method for preparing high content soybean oligosaccharide by simulated moving bed separation
  • Method for preparing high content soybean oligosaccharide by simulated moving bed separation
  • Method for preparing high content soybean oligosaccharide by simulated moving bed separation

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Embodiment Construction

[0016] Specific implementation examples

[0017] The invention provides an industrially feasible method for purifying soybean oligosaccharides. The method uses simulated moving bed chromatographic separation technology, continuous feed and continuous discharge, and has the characteristics of high degree of automation, simple operation, high separation strength, high concentration of separated products, and high content of main products.

[0018] According to the method of the present invention, the used separating agent is calcium type or sodium type cation exchange resin or porous calcium-Y type molecular sieve, all of which are sold in the market and have very stable performance. The eluent used is purified water, which is economical and readily available.

[0019] According to the method of the present invention, a simulated moving bed chromatographic separation device equipped with the above-mentioned separating agent is used. The separation device is composed of 4 or 6 ...

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Abstract

The invention discloses a method for purifying soybean oligosaccharides. The soybean oligosaccharides with a content of 40% are separated by a simulated moving bed chromatographic separation device at a separation temperature of 45-80°C, using a cation exchange resin as an adsorbent, and using purified water as an eluent. Two kinds of products are obtained: one is the high-purity soybean oligosaccharide liquid with a soybean oligosaccharide (stachyose and raffinose) content greater than 85%, which is the main product; the other is a disaccharide and monosaccharide-containing component The fructose solution is a by-product. The yield of soybean oligosaccharides is greater than 90%.

Description

Technical field: [0001] The invention relates to an oligosaccharide purification technology, in particular to a method for purifying oligosaccharides by using simulated moving bed technology. technical background: [0002] Soy oligosaccharides are the general term for soluble sugars extracted from soybeans. The main components are stachyose, raffinose, sucrose, glucose, fructose, etc. Among them, stachyose and raffinose are oligosaccharides, which have the characteristics of oligosaccharides , is the proliferation factor of bifidobacteria, which has the effects of inhibiting the growth of harmful bacteria, promoting gastrointestinal motility, and preventing constipation. Soy oligosaccharides are widely used in food and health products due to the characteristics of stachyose and raffinose. [0003] The content of oligosaccharides (stachyose, raffinose) in the currently commercially available soybean oligosaccharides is about 40%, and other components are sucrose (10-15%), glu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H3/06C07H3/02C07H3/04B01D15/10B01D15/36
Inventor 李瑛金树人周日尤伍玉碧
Owner 南京凯通粮食生化研究设计有限公司
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