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Collagen-based composite cornea substitute with bioactivity and preparation method thereof

A bioactive and substitute technology, applied in eye implants, medical science, prostheses, etc., can solve problems such as easy hydrolysis, MPC easy to absorb moisture, etc., achieve good tissue compatibility, promote enrichment, and improve stability sexual effect

Inactive Publication Date: 2012-10-10
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the MPC used is extremely hygroscopic and easily hydrolyzed

Method used

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  • Collagen-based composite cornea substitute with bioactivity and preparation method thereof
  • Collagen-based composite cornea substitute with bioactivity and preparation method thereof
  • Collagen-based composite cornea substitute with bioactivity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The ratio of each component of the bioactive composite corneal substitute is as follows:

[0033] Pig skin type I collagen: MPDSAH (mass ratio) 3:1

[0034] MPDSAH: PEGDA (mass ratio) 2:1

[0035]Collagen: EDC: NHS (molar ratio) 1:1:1

[0036] The steps of preparing the bioactive composite corneal substitute with the above-mentioned components are as follows:

[0037] (1) Preparation of composite corneal substitute: get 0.5g of 13.7% pigskin type I collagen solution into a syringe, and connect another syringe through a T-shaped container to seal; take MPDSAH with a mass ratio of 1:3 to collagen and dissolve it in In water, use a microsyringe to move into the T-shaped container through the sealing gasket, repeatedly push and pull to mix evenly, and then use a microsyringe to inject the cross-linking agent EDC, NHS and photoinitiator Irgacure 2959 in sequence, wherein EDC:NHS:collagen=1:1:1( molar ratio), initiator Irgacure2959:MPDSAH=1:25 (molar ratio), push and pull ...

Embodiment 2

[0041] The ratio of each component of this biologically active composite corneal substitute is as follows:

[0042] Pig skin type I collagen: MPDSAH (mass ratio) 2:1

[0043] MPDSAH: PEGDA (mass ratio) 2:1

[0044] Collagen: EDC: NHS (molar ratio) 1:1:1

[0045] The steps of preparing the bioactive composite corneal substitute with the above-mentioned components are as follows:

[0046] (1) Preparation of composite corneal substitute: get 0.5g of 13.7% pigskin type I collagen solution into a syringe, and connect another syringe through a T-shaped container to seal; take MPDSAH with a mass ratio of 1:2 to collagen and dissolve in In water, use a microsyringe to move into the T-shaped container through the sealing gasket, repeatedly push and pull to mix evenly, and then use a microsyringe to inject the cross-linking agent EDC, NHS and photoinitiator Irgacure 2959 in sequence, wherein EDC:NHS:collagen=1:1:1( molar ratio), initiator Irgacure2959:MPDSAH=1:25 (molar ratio), push ...

Embodiment 3

[0050] The ratio of each component of this biologically active composite corneal substitute is as follows:

[0051] Pig skin type I collagen: MPDSAH (mass ratio) 1:1

[0052] MPDSAH: PEGDA (mass ratio) 2:1

[0053] Collagen: EDC: NHS (molar ratio) 1:1:1

[0054] The steps of preparing the bioactive composite corneal substitute with the above-mentioned components are as follows:

[0055] (1) Preparation of composite corneal substitute: get 0.5g of 13.7% pigskin type I collagen solution into a syringe, and connect another syringe through a T-shaped container to seal; take MPDSAH with a mass ratio of 1:1 to collagen and dissolve In water, use a microsyringe to move into the T-shaped container through the sealing gasket, repeatedly push and pull to mix evenly, and then use a microsyringe to inject the cross-linking agent EDC, NHS and photoinitiator Irgacure 2959 in sequence, wherein EDC:NHS:collagen=1:1:1( molar ratio), initiator Irgacure2959:MPDSAH=1:25 (molar ratio), push and...

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Abstract

The invention provides a collagen-based composite cornea substitute with bioactivity and a preparation method thereof. The composite cornea substitute is prepared by the following steps: taking collagen and 3-(methacrylamide)propyl-dimethyl(3-sulfopropyl)amide (MPDSAH) as raw materials and water as a solvent, crosslinking the collagen with zero-length cross linker carbodiimide (EDC); taking Irgacure 2959 as a photo initiator and polyethyleneglycol diacrylate (PEGDA) as a crosslinking agent to perform initiated polymerization and crosslinking for the MPDSAH; and after even mixing, injecting a die with the mixture for cure forming. After freeze drying, the composite cornea constitute has a three-dimensional porous structure, can adsorb a large amount of cornea cell growth factors, can be stored for a long time, and can recover the primary shape and performance like before freeze drying after water absorption and expansion. The prepared composite cornea substitute has good histocompatibility, can induce and promote the cornea regeneration, and can be biodegraded along with the cornea regeneration, and the introduction of the growth factors can promote the enrichment of self growth factors and reduce the occurrence of postoperative complications.

Description

technical field [0001] The invention relates to a biologically active composite corneal substitute and a preparation method thereof, belonging to the corneal transplant material technology in the field of tissue engineering. Background technique [0002] Corneal disease is a blinding eye disease with high incidence and difficult treatment. At present, corneal transplantation is the main treatment for corneal diseases, but the lack of corneal donor sources and high postoperative rejection rate limit the clinical application of corneal transplantation. Therefore, researchers began to try to use corneal substitutes to help corneal patients restore vision. Since 1859, Heusser implemented artificial corneal implantation for human for the first time, and the development of artificial cornea has a history of more than 100 years. Polymethyl methacrylate (PMMA), silicone rubber, polyhydroxyethyl methacrylate (PHEMA) and fluorocarbon polymers have been successfully used as materials...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/54A61L27/56A61F2/14A61L27/24A61L27/18
Inventor 刘文广梁爽王鹏飞
Owner TIANJIN UNIV
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