Method for preparing bacillus subtilis preparation

A technology of Bacillus subtilis and a production method, which is applied in the field of production of microbial preparations, can solve the problems of high production cost, low content of active ingredients, short shelf life and the like, and achieves the effects of low cost, long shelf life and not easy to absorb moisture

Active Publication Date: 2009-10-21
德强生物股份有限公司
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AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a method for preparing a Bacillus subtilis preparation in order to solve the problems of high production cost, low active ingredient content and short shelf life in the existing preparation methods of the Bacillus subtilis preparation

Method used

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Experimental program
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Effect test

specific Embodiment approach 1

[0006] Specific Embodiment 1: The production of Bacillus subtilis preparation according to the present embodiment is carried out according to the following steps: 1. Fermentation liquid is obtained after Bacillus subtilis B10 is fermented; , the operating pressure is 3-6bar, the temperature is 20-45°C, the membrane flux is 55-65LMH, the solution passing through the ultrafiltration membrane is antibiotic solution, the solution trapped on the liquid inlet side of the membrane is spore solution, and the concentration of spore solution 5~7 times, the washing consumption of water is 20%~40% of fermented liquid volume; The temperature is 10~26bar, the temperature is 20~45°C, the membrane flux is 45~55LMH, and the concentration factor is 7~10 times, wherein the solution passing through the ultrafiltration membrane is antibiotic permeate; 4. Permeate the antibiotic in step 3 The liquid is filtered through a nanofiltration membrane with a cut-off molecular weight of 400-600k to obtain ...

specific Embodiment approach 2

[0015] Embodiment 2: This embodiment differs from Embodiment 1 in that in step 1, Bacillus subtilis B10 is inoculated into a fermenter for fermentation and cultivation, the fermentation temperature is 36-39° C., and the fermentation time is 65-75 hours. Other steps and parameters are the same as those in Embodiment 1.

[0016] In this embodiment, after the Bacillus subtilis B10 is fermented and cultured, the effective viable spore content in the fermentation broth is 20-40 billion / g.

specific Embodiment approach 3

[0017] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the concentration ratio of the spore solution in step two is 6 times. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a method for preparing a bacillus subtilis preparation, and relates to a method for preparing a microorganism preparation. The method solves the problems of the prior method for preparing bacillus subtilis preparation such as high preparation cost, and low content of effective ingredients and short preservation period of prepared product. The method comprises the following steps that: 1, bacillus subtilis B10 is fermented to obtain fermentation broth; 2, the fermentation broth is subjected to ultrafiltration membrane separation to obtain antibiotic solution and germ solution; 3, the antibiotic solution is subjected to ultrafiltration membrane filtration to obtain antibiotic permeate liquid; 4, the antibiotic permeate liquid is subjected to nanofiltration membrane filtration once again to obtain antibiotic concentrated liquid; and 5, the antibiotic concentrated liquid and the germ solution in the step two are mixed into powder through spray drying so as to obtain the bacillus subtilis preparation. The preparation method has low cost, and the prepared product has good quality and long shelf life, and can be stored for over 2 years without deterioration under the sealing condition.

Description

technical field [0001] The invention relates to a preparation method of a microbial preparation. Background technique [0002] Bacillus subtilis preparation is a broad-spectrum insecticide of live fungi, and its main components are antibiotics and spores of Bacillus subtilis. It is a new type of bio-environmental protection pesticide. Botrytis cinerea, vegetable powdery mildew, wheat powdery mildew, etc. have excellent control effects. Bacillus subtilis preparation is a bio-pesticide that develops green food, pollution-free agricultural products, and protects the environment at home and abroad. Farmland is widely used. Now, the Bacillus subtilis B10 described in "Inhibitory effect of Bacillus subtils (Bacillus subtils) B10 on post-harvest strawberry fruit diseases" published by Zhao Yan on the "Journal of Fruit Trees" in 2007 is mostly used to make Bacillus subtilis Preparation, the traditional method is: after fermenting and cultivating Bacillus subtilis B10, the ferment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00C12P1/04C12R1/125
Inventor 孙新宇李宏园田莉王敏张龙丰刘伟
Owner 德强生物股份有限公司
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