Earthworm protein polypeptide preparation
A ground dragon protein and preparation technology, which is applied in the fields of peptide/protein components, medical preparations containing active ingredients, cardiovascular system diseases, etc. High concentration, increase blood pressure, and enhance the effect of activity
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Embodiment 1
[0036] The preparation of embodiment 1 earthworm protein polypeptide preparation
[0037]① Take 100g of Earthworm, add 500ml of double distilled water, homogenate for 30 minutes, centrifuge at 15000r / min for 30 minutes at 4°C, take the supernatant of Earthworm after centrifugation, and set aside;
[0038] ②Take 60ml of the earthworm supernatant (containing about 74.4mg of protein) obtained in step ①, add 0.7mg of trypsin at a concentration of 250U / mg, 0.7mg of chymotrypsin at a concentration of 1000U / mg and elastin at a concentration of 30U / mg Protease 0.7mg, make the weight percentage of the mixed enzyme reach 3%, then add sodium hydroxide to adjust the pH value to 8.0, and enzymatically hydrolyze in a water bath at 50°C for 5 hours;
[0039] ③ Take the enzymatic hydrolyzate obtained in step ② and a pharmaceutically acceptable carrier to prepare the terrapenin polypeptide preparation.
[0040] The enzymatic hydrolyzate of the ground dragon protein polypeptide obtained in the...
Embodiment 2
[0041] The preparation of embodiment 2 earthworm protein polypeptide preparations
[0042] ① Take 100g of Earthworm, add 300ml of double distilled water, homogenate for 10 minutes, centrifuge at 11000r / min for 20 minutes at 4°C, take the supernatant of Earthworm after centrifugation, and set aside;
[0043] ②Take 60ml of the earthworm supernatant obtained in step ① (containing about 74.4mg of protein), add 1.5mg of trypsin at a concentration of 200U / mg, 1mg of chymotrypsin at a concentration of 800U / mg and elastase at a concentration of 20U / mg 1.2mg, make the weight percentage of the mixed enzyme reach 5%, then add sodium hydroxide to adjust the pH value to 8.0, and enzymatically hydrolyze in a water bath at 50°C for 2 hours;
[0044] ③ Take the enzymatic hydrolyzate obtained in step ② and a pharmaceutically acceptable carrier to prepare the terrapenin polypeptide preparation.
[0045] The enzymatic hydrolyzate of the ground dragon protein polypeptide obtained in the present ...
Embodiment 3
[0046] The preparation of embodiment 3 earthworm protein polypeptide preparations
[0047] ① Take 100g of Earthworm and add 300ml of distilled water, homogenate for 20 minutes, centrifuge at 12000r / min for 30 minutes at 4°C, take the supernatant of Earthworm after centrifugation, and set aside;
[0048] ②Take 100ml of the earthworm supernatant obtained in step ① (containing about 92.5 grams of protein), add 0.9mg of trypsin at a concentration of 250U / mg, 0.9mg of chymotrypsin at a concentration of 1000U / mg and elastase at a concentration of 30U / mg Protease 0.9mg, make the weight percentage of the mixed enzyme reach 3%, then add sodium hydroxide to adjust the pH value to 8.0, and enzymatically hydrolyze in a 50°C water bath for 5 hours;
[0049] ③Take 100ml of the enzymolysis solution obtained in step ②, under the conditions of the temperature of the enzymolysis solution at 50°C, the operating pressure at 0.25MPa, and the surface speed at 200r / min, use pore diameters of 50,000,...
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