Method for extracting purified cortex phellodendri total alkaloid by utilizing ion-exchange fiber

An ion-exchange fiber and total alkaloid technology, applied in the field of medicine, can solve problems such as unseen extraction and purification of Cork, and achieve the effects of significant energy saving, improved product purity and yield, and high fiber utilization.

Inactive Publication Date: 2009-12-30
BEIJING INSTITUTE OF CLOTHING TECHNOLOGY
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Problems solved by technology

[0005] Phellodendron chinense is the dry bark of Phellodendron chinense Schneid or P. amurense Rupr., the former is commonly called "Sichuan Phellodendron" and the latter is commonly called "Guan Phellodendron". , purging fire and steaming, detoxifying and treating sores. Modern pharmacological studies ha

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  • Method for extracting purified cortex phellodendri total alkaloid by utilizing ion-exchange fiber

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Embodiment 1

[0036] Embodiment 1: Phellodendron Phellodendri total alkaloid extract purification process

[0037] Get Phellodendron Phellodendri powder 10g, add 70% methanol 100mL, extract 2 times, extract 3 hours each time, acidity value is 1: 100 (hydrochloric acid v: methanol v), reclaim solvent under reduced pressure, vacuum-dry to obtain Phellodendron Phellodendri total alkaloids crude extract , 12.1% in terms of total alkaloid content. The extract is dispersed and dissolved by adding 70% ethanol, so that the solution concentration is 1.314 mg / mL (in terms of total alkaloids), and the pH value is 4.8. The supernatant is passed through 0.5 g of strongly acidic cation exchange fibers with a bed volume of 5 mL. Column adsorption, the adsorption flow rate is 8BV h -1 . Use a flow rate of 10BV / h and 2BV water to elute and remove impurities, then use 70% ethanol (PH=13) for 12BV to elute at a flow rate of 10BV·h -1 The eluent was eluted, the eluent was collected, the organic solvent was ...

Embodiment 2

[0039] Embodiment 2: Phellodendron Phellodendri total alkaloid extract purification process

[0040]Get 117.5g of Cortex Phellodendri powder, add 1175mL of 70% methanol, extract 2 times, extract 3 hours each time, the acidity value is 1: 100 (hydrochloric acid v: methanol v), recover the solvent under reduced pressure, and vacuum dry to obtain the crude extraction of total alkaloids of Cortex Phellodendri substances, the total alkaloid content is 11.3%. The extract was dissolved in 90% ethanol to make the solution concentration 1.69mg / mL and the pH value 5.1. Take the supernatant and adsorb it through a 6.0g strong acid cation exchange fiber column with a bed volume of 60mL. The adsorption flow rate was 5BV h -1 . Use a flow rate of 10BV / h, 2BV water elution to remove impurities, and then use 80% ethanol + 1mol / l NaOH mixed solution (volume ratio 5:1) 10BV elution, the elution flow rate is 10BV h -1 , collect the ethanol eluent, recover the solvent, and dry under reduced pre...

Embodiment 3

[0042] Embodiment 3: Phellodendron Phellodendri total alkaloid extract purification process

[0043] Get 200g of Phellodendron Phellodendri powder through a 40-mesh sieve, add 2000mL of 70% methanol, extract twice at a pH of 1 and 50°C for 3 hours each time, recover the solvent under reduced pressure, and dry in vacuo to obtain 27.83g of powder. The purity of 13.9% is 15.4% based on the total alkaloid content. The extract was dissolved in 80% methanol to make the solution concentration 1.69 mg / mL, pH = 5, take the supernatant, and adsorb it through a 12.0 g, 120 mL strongly acidic cation exchange fiber column with an adsorption flow rate of 8 BV h -1 . Use a flow rate of 10BV / h, 2BV water elution to remove impurities, and then use a mixture of 95% ethanol + 1mol / L NaOH (volume ratio 5:1) 8BV elution, the elution flow rate is 12BV h -1 , collect the ethanol eluent, recover the solvent, and dry under reduced pressure, which is the total alkaloid extract of Phellodendron Phello...

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Abstract

The invention relates to a method for extracting purified plant medical component-total alkaloid by utilizing ion-exchange fiber, which comprises the following steps: cleaning, drying and crushing the raw cortex phellodendri for decoction and leaching; adsorbing the leached solution by an ion-exchange fiber column under the condition that the consistency of total alkaloid is 0.5-6.0 g.L <-1>, the temperature is 10-80 DEG C, the flow rate is 0.5-20.0 BV.h<-1>(BV: column volume ) and the pH value is 1.0 to 6.0; eluting the adsorbed solution by eluent prepared from 2 to 10 of water, alcohol and ester or the mixture thereof and 1 of pH regulator by volume ratio, wherein the eluting temperature is 10-90 DEG. C, the flow rate is 0.2-20 BV.h<-1>, the water, alcohol and ester or the mixture thereof is 5-100 percent of 4-20 times of column volume (BV), and the acid concentration of the pH regulator is 1* 10 <-4> to 3.0 mol <-1>; and collecting the eluate, and recovering the organic matters to obtain total alkaloid powder. With the advantages of perfect equipment, simple operation process, high fiber utilization ratio, low production cost, high processing efficiency, obvious energy saving effect and controllable product quality, the method enhances the product purity and yield and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for extracting, separating and purifying natural plant medicinal components with ion-exchange fibers, in particular to a method for extracting and purifying total alkaloids in Cortex Phellodendri with ion-exchange fibers. Background technique [0002] Ion Exchange Fiber (IEF) is a fibrous ion exchange adsorption material, which is composed of many monofilaments with uniform thickness, and its diameter ranges from 20 to 300 μm. It itself contains fixed ions and mobile ions with the opposite sign to the fixed ions. When in contact with a solution capable of dissociating compounds, the active ions of the ion exchange fiber are exchanged with ions of the same sign in the solution, so it is called ion exchange fiber. Compared with granular ion exchangers, ion exchange fibers have the characteristics of large specific surface, small diameter, fast adsorption and desorption, la...

Claims

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Application Information

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IPC IPC(8): A61K36/756A61K129/00
Inventor 刘廷岳聂素双崔成民周绍箕
Owner BEIJING INSTITUTE OF CLOTHING TECHNOLOGY
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