Prolactin time-resolved fluoroimmunoassay method and kit

An immunofluorescence, time-resolved technology, applied in the analysis of materials, fluorescence/phosphorescence, and material excitation analysis, etc., can solve the problems of polluted environment, low sensitivity, poor stability, etc., and achieve small cross-reaction, good specificity, and short detection time Effect

Inactive Publication Date: 2009-12-30
PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a time-resolved immunofluorescence analysis method and kit for PRL detection, which mainly solve the technical problems of low sensitivity, poor stability, cumbersome operation and environmental pollution in the prior art.

Method used

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  • Prolactin time-resolved fluoroimmunoassay method and kit
  • Prolactin time-resolved fluoroimmunoassay method and kit
  • Prolactin time-resolved fluoroimmunoassay method and kit

Examples

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Embodiment

[0031] Example: Prolactin (PRL) Determination

[0032] 1. Preparation of standard products

[0033] Use 6% BSA, 4% sugar, 0.1% NaN3, 50mmol / L Tris-HCl buffer solution to prepare PRL protein into 0, 3, 10, 30, 100, 300mIU / mL standard solution, and calibrate with national PRL standard. Aliquot and store at +2~+8℃.

[0034] 2. Operation steps

[0035] 1) Preparation of reagents

[0036] a) Washing liquid: Mix 40mL of concentrated washing liquid and 960mL of deionized water in a clean washing liquid bottle, and use it as a working washing liquid for later use.

[0037] b) Europium labeling: within one hour before use, dilute the europium label 1:20 with assay buffer.

[0038] c) Equilibrate the kit analysis buffer, the sample to be tested, the calibrator and the required number of microwell reaction strips to room temperature (20-25° C.).

[0039] 2) Pipette 25 μl of calibrator or sample, and add to the bottom of the corresponding microwell in sequence.

[0040] 3) Add 100 μ...

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Abstract

The invention discloses a prolactin (PRL) time-resolved fluoroimmunoassay method and a kit. The method comprises the following steps: selecting and using a monoclonal antibody against PRL as a coated antibody, and diluting sodium carbonate buffer solution to 1 to 10mu g/ml as coating liquid; preparing a lanthanide ion label against the PRL monoclonal antibody as a labeled antibody; diluting and using the reaction buffer solution according to a ratio of 1 to 20 during experiment; and on a reaction plate of the coated antibody, adding 25mu l of standard substance of PRL or sample to be detected and the diluted labeled antibody into each hole in turn, and carrying out fluorescence detection after hatching. The invention also provides the corresponding kit. The method and the kit have the advantages of high sensitivity, specificity and stability; and the high automated assay system can improve the speed of clinical examination result, greatly reduce human error and increase the reliability of detected result.

Description

Technical field: [0001] The invention relates to a prolactin (PRL) quantitative detection kit, which belongs to the detection field of time-resolved immunofluorescence analysis. Background technique: [0002] Prolactin (PRL) is a single-chain polypeptide hormone secreted by the anterior pituitary gland. It is composed of 199 amino acids and has a molecular weight of about 23Kd. Its structure and biology are similar to growth hormone and placental lactin. Male , female body contains. Like GH, PRL does not need to act through target glands. It plays a very important role in the growth and development of the body and mammary gland, milk secretion, gonad development, regulation of water and electrolyte metabolism. The synthesis and release of prolactin are controlled by neuroendocrine, mainly regulated by prolactin releasing factor (PRF) and prolactin inhibitory factor (PIF) (mainly PIF). The basal level of prolactin in women is slightly higher than that in men, and it increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/577G01N21/64
Inventor 沈君吴冯波
Owner PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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