Method for detecting related gene mutations of diabetes drug treatment as well as special chip and kit thereof
A diabetes drug and gene technology, applied in the field of gene analysis, can solve the problems of "all or none" detection signal and short probes, etc.
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[0073] [Example 1] Preparation of a dual-probe diabetes drug treatment-related gene mutation chip detection system based on allele-specific amplification.
[0074] 1. Preparation process
[0075] See attached Figure 4 .
[0076] 2. The main raw and auxiliary materials and equipment required for preparation
[0077] Table 6
[0078] Reagents and Instruments
place of origin
Reagent Purity / Concentration
Blank Ultraflat
Shanghai Baiao Technology Co., Ltd.
/
Aminosilane reagent
Acros (United States)
99wt.%
Acros (United States)
25wt.%
95% ethanol
Shanghai Chemical Plant
Analytical pure
Glacial acetic acid
Shanghai Chemical Plant
Analytical pure
NaBH 4
Shanghai Chemical Plant
Analytical pure
Na 2 HPO 4
Shanghai Chemical Plant
Analytical pure
K H 2 PO 4
Shanghai Chemical Plant
Analytical pure
Na 2 CO 3
S...
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