Decision method of hybridization reaction starting point of leakage-noise surface wave (LSAW) nucleic acid sensor

Abstract

The invention discloses a decision method of a hybridization reaction starting point of a leakage-noise surface wave (LSAW) nucleic acid sensor. The decision method comprises the following steps: using an orthogonal wavelet basis to carry out the 6-scale wavelet decomposition of phase signals acquired by a sensor; finding a wavelet transform modulus maximum point from the first scale detail signal d1; and judging the modulus maximum point as the hybridization reaction starting point if the modulus maximum point has corresponding modulus maximum points in other five scale detail signals d2-d6.The invention provides a uniform, objective and scientific method for judging the hybridization reaction starting point of the LSAW sensor, avoids personal errors caused by subjective factors in traditional visual measurement, greatly improves the detection accuracy, the repeatability and the sensitivity of the sensor, has the advantages of simplicity, rapidness, real-time property and important practical application values and lays a solid foundation for the programming of later automatic judging software and the clinical application of the sensor.

Description

technical field [0001] The invention relates to a method for judging the starting point of a hybridization reaction of a nucleic acid sensor, in particular to a method for judging the starting point of a hybridization reaction of a leaky surface acoustic wave (LSAW) nucleic acid sensor. Background technique [0002] With the development of molecular biology techniques, rapid gene detection has become one of the important means for early diagnosis of clinical etiology. Nucleic acid sensors will play an important role in the rapid detection of genes due to their high efficiency, sensitivity, specificity, compact structure, economical and practical characteristics. [0003] The LSAW nucleic acid sensor uses the special tangential direction of the piezoelectric crystal (such as cutting 36 degrees in the Y direction and propagating LSAW in the X direction) to excite LSAW. When the mass load on the surface of the LSAW propagation path changes slightly, the probe and target fixed o...

AI Technical Summary

Problems solved by technology

Due to the differences in the sampling force and technique of different operators, the phase curve of the sensor usually shows diversity at the initial stage of the hybridization reaction, specifically, there is a sample addition shock peak or no sample addition shock peak, and there is no obvious starting point of the symbolic reaction. This makes it difficult to determine the starting point of the hybridization reaction.

In addition, the determination of the starting point of the traditional hybridization reaction mainly relies on visual inspection, which often causes human errors in the test results, thus greatly affecting the accuracy and repeatability of the test results.

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