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Production method for microbe flocculant

A microbial flocculant and production method technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of long production cycle, difficult operation and management, and long cultivation time of microbial flocculants, and achieve good removal Turbidity and decolorization, easy operation, less sediment effect

Inactive Publication Date: 2010-02-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method is complex in process, not easy to operate and manage, and the cultivation time of the bacteria is long, and the production cycle of the microbial flocculant is long.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of production method of microbial flocculant, medium raw material formula, calculate with the concentration in raw material of composition, be glucose 6g / L, peptone 1.20g / L, potassium dihydrogen phosphate 1.8g / L, dipotassium hydrogen phosphate 4.8g / L L, Magnesium Sulfate 180mg / L, Sodium Chloride 19mg / L, Ferrous Sulfate 250mg / L, adjust pH7.0 with NaOH, then in volume ratio, according to the inoculum size of 1.5:100, insert fluorescent fake Monocellular bacteria (photosynthetic bacteria liquid produced by Zhongshan Baihao Bioengineering Co., Ltd.), the rotation speed of the shaker is 200rpm, the temperature is 25°C, cultivated for 38h, stop stirring, wait for the precipitation, pour the supernatant, and the precipitation is The crude product of the required microbial flocculant; it has been determined that its main component is polysaccharide, which is secreted during the growth of the bacteria. Take 1L of sewage from the Pearl River, add 0.25mg of the crude floccu...

Embodiment 2

[0029] A kind of production method of microbial flocculant, calculates with the concentration in raw material of composition, and medium raw material formula is glucose 6.5g / L, peptone 1.50g / L, potassium dihydrogen phosphate 2.0g / L, dipotassium hydrogen phosphate 4.5g / L L, magnesium sulfate 200mg / L, sodium chloride 20mg / L, ferrous sulfate 230mg / L, adjust the pH to 7.0 with NaOH, and then insert the fluorescent fake into the culture medium according to the volume ratio of 1.5:100. Monocellular bacteria (photosynthetic bacteria liquid produced by Zhongshan Baihao Biological Engineering Co., Ltd.), in a shaker with a rotation speed of 200rpm and a temperature of 25°C, cultured for 38h, stopped stirring, and waited for its precipitation, which was the crude product of the required microbial flocculant ; After determination, its main component is polysaccharide, which is secreted during the growth of the bacteria. Take 1L of sewage discharged from the canteen, add 0.20 mg of the cr...

Embodiment 3

[0032] A kind of production method of microbial flocculant, calculate with the concentration in raw material of composition, medium raw material formula is glucose 7.0g / L, peptone 1.0g / L, potassium dihydrogen phosphate 1.8g / L, dipotassium hydrogen phosphate 5.0g / L L, magnesium sulfate 190mg / L, sodium chloride 18mg / L, ferrous sulfate 250mg / L, adjust pH 6.5 with NaOH, and then add fluorescent false in the culture medium according to the volume ratio, according to the inoculum size of 1.5:100 Monocellular bacteria, cultured for 38 hours at a shaking table with a rotation speed of 200rpm and a temperature of 25°C, stopped stirring, and waited for its precipitation. The precipitation was the crude product of the required microbial flocculant; it was determined that its main component was polysaccharide, which was the main component of the bacterial flocculant during the growth process. Secreted. Take the sewage 11 discharged from the paper mill, add 0.30 mg of the flocculant crude ...

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Abstract

The invention discloses a production method for microbe flocculant, which comprises the following steps: eudomonas fluoresce is inoculated into culture medium by volume ratio according to inoculum size of 1.5-3.0:100 and cultured for 30-48h in the temperature of 25-30 DEG C to prepare microbe flocculant; according to the concentration of formulation constituents in culture medium, the raw materialformulation of the culture medium is as follows: 5-8g / L amylaceum, 0.50-1.75g / L of peptone, 0.5-2.0g / L of monopotassium phosphate, 0.5-5.0g / L of dipotassium phosphate, 100-300mg / L of magnesium sulfate, 200-700mg / L of sodium chloride, and 10-60mg / L of ferrous sulphate; the NaOH is used for regulating Ph to be 6.0-8.0. The microbe flocculant produced by the method can obtain high flocculating rateand few adding amount; and the microbe flocculant is used for water treatment, thus being efficient, simple, convenient, and cheap.

Description

technical field [0001] The invention relates to a microbial flocculant, in particular to a production method of the microbial flocculant. Background technique [0002] With the development of the global economy and the progress of industrial production, the living standards of human beings have been greatly improved, but the environment on which human beings depend is being increasingly destroyed and the laws of the environment are disordered. The severe situation of scarcity of water resources and aggravation of pollution has promoted the research and application of water treatment agents. In the history of the development of water treatment agent flocculation technology, inorganic salt flocculants (such as aluminum sulfate, aluminum chloride, ferric chloride, etc.) were developed in the 1960s, but the treatment effect was not ideal; Agents (such as polyaluminum chloride, polyferric sulfate, etc.) have received good results in water treatment, but the aluminum ions of poly...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/39
Inventor 林炜铁李会琴昝继清罗剑飞
Owner SOUTH CHINA UNIV OF TECH
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