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Method for extracting linolenic acid from purslane

A technology of purslane and linolenic acid, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of sharp decline in provenance, neglect of seed retention, and reduction of purslane provenance, so as to reduce solvent The effect of reducing the loss, improving the extraction effect, and shortening the extraction time

Inactive Publication Date: 2010-02-10
陈海燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is the lack of systematic and large-scale research and development
The second is the sharp decline in seed sources, which affects sustainable development
When people harvest purslane, they sometimes uproot it and do not pay attention to saving seeds, which reduces the source of purslane and affects sustainable development
The third is that the development form is primitive and simple, and there are few professional wild vegetable processing plants
Therefore, the added value of the product is small, it is difficult to form a scale, and it lacks market competitiveness
The fourth is the lack of understanding of purslane
Five is the lack of provenance supply and cultivation technology
Sixth, the market development of purslane is not enough
However, most of the purslane in our province is exported after simple primary processing, and its comprehensive benefits are obviously not high, and the added value needs to be greatly improved. It also has a great industrial radiation effect on regional economic development
[0007] At present, there have been relevant reports on the extraction and utilization of purslane. Hou Yinhuan et al. took the transfer rate of a-linolenic acid in medicinal materials, the yield of extract and the content of linolenic acid in the extract as the investigation indicators, and extracted it with absolute ethanol. Linolenic acid in purslane, but there is no relevant report on the extraction of linolenic acid in purslane by ultrasonic reflux

Method used

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  • Method for extracting linolenic acid from purslane
  • Method for extracting linolenic acid from purslane
  • Method for extracting linolenic acid from purslane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: A method for extracting linolenic acid from purslane, comprising the following steps: crushing dried purslane stems and leaves or one of them with a pulverizer to 50-200 mesh. Weigh 5.0g of the powder into a 250mL round bottom flask; add 100ml of organic solvent, and ultrasonically reflux for 4 hours under the condition of ultrasonic temperature of 40°C and ultrasonic power of 990W; The extract is the obtained linolenic acid mixture. The extract was concentrated under reduced pressure and dried to obtain a crude product of linolenic acid.

[0024] After the extract was concentrated by rotary evaporation, the remaining substance after concentration was dissolved with n-hexane and the volume was adjusted to 10 mL. The 10 mL sample was methylated and detected by reversed-phase high performance liquid chromatography. The results are shown in Table 1.

[0025] Table 1 The influence of different organic solvents on the extraction of linolenic acid

[0026] ...

Embodiment 2

[0027] Embodiment 2: A method for extracting linolenic acid from purslane, comprising the following steps: crushing dried purslane stems and leaves or one of them with a pulverizer to 50-200 mesh. Weigh 5.0g of the powder into a 250mL round bottom flask; add 50ml of organic solvent, and ultrasonically reflux for 2 hours under the condition of ultrasonic temperature of 50°C and ultrasonic power of 330W; The extract is the obtained linolenic acid mixture. The extract was concentrated under reduced pressure and dried to obtain a crude product of linolenic acid.

[0028] After the extract was concentrated by rotary evaporation, the remaining substance after concentration was dissolved with n-hexane and the volume was adjusted to 10mL. The 10mL sample was methylated and detected by reversed-phase high performance liquid chromatography. The results are shown in Table 2.

[0029] Table 2 Effect of different organic solvents on the extraction of linolenic acid

[0030]

Embodiment 3

[0031] Embodiment 3: A method for extracting linolenic acid from purslane, comprising the following steps: crushing dried purslane stems and leaves or one of them with a pulverizer to 50-200 mesh. Weigh 5.0g of the powder into a 250mL round bottom flask; add 25ml of organic solvent, and ultrasonically reflux for 0.5 hours under the conditions of ultrasonic temperature of 70°C and ultrasonic power of 220W; The extract is the obtained linolenic acid mixture. The extract was concentrated under reduced pressure and dried to obtain a crude product of linolenic acid.

[0032] After the extract was concentrated by rotary evaporation, the remaining substance after concentration was dissolved with n-hexane and the volume was adjusted to 10 mL. The 10 mL sample was methylated and detected by reversed-phase high performance liquid chromatography. The results are shown in Table 3.

[0033] Table 3 The influence of different organic solvents on the extraction of linolenic acid

[0034] ...

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Abstract

The invention relates to a method for extracting linolenic acid from purslane, comprising the following steps: a) adding the smashed purslane into organic solvent, the proportion between the gram of the purslane and the milliliter of the organic solvent is 1:5-20, wherein the organic solvent is one of petroleum ether, ethyl acetate, acetone and normal hexane; b) under the auxiliary processing of the ultrasonic wave, inversely flowing and extracting for 0.5-4 h, wherein the extracting temperature is 40-70 DEG C; and c) filtrating and removing the slag, obtaining extracting solution, concentrating the extracting solution by means of pressure reduction, and drying to obtain the crude product of the linolenic acid. The method has simple operation, short extraction time, low production cost, and high security, wherein the ultrasonic wave auxiliary process extremely and obviously improves the extracting effect of the linolenic acid from the purslane.

Description

technical field [0001] The invention relates to a method for extracting linolenic acid from purslane. Background technique [0002] Black wood purslane (Portulaca oleracea L.) is a plant of the genus Portulaca oleracea, also known as purslane, five-element grass, long-lived vegetable, ant vegetable, etc. In temperate and tropical regions of the world, it is a medicinal and edible plant. "Pharmacopoeia of the People's Republic of China" records that the dried aerial part of purslane is used as medicine, which has the functions of clearing away heat and detoxification, cooling blood and stopping bleeding. Modern pharmacological studies have shown that purslane has antibacterial, blood lipid lowering, anti-aging, muscle relaxation, anti-inflammatory, analgesic and wound healing effects. Purslane is a traditional medicinal and edible plant, which is rich in nutrients and contains various chemical components such as alkaloids, phenols, organic acids and terpenes. At present, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C57/12C07C51/48
Inventor 陈海燕
Owner 陈海燕
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