Method for measuring general ginsenoside by anthrone colorimetric method

A colorimetric technology of total ginsenosides and anthrone, which is applied in the preparation of test samples, measurement of color/spectral characteristics, etc.

Active Publication Date: 2010-02-17
WUXI JIANTE PHARM CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there is no report on the determination of total ginsenosides by the method of the present invention both at home and abroad

Method used

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  • Method for measuring general ginsenoside by anthrone colorimetric method
  • Method for measuring general ginsenoside by anthrone colorimetric method
  • Method for measuring general ginsenoside by anthrone colorimetric method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Determination of total ginsenosides in a ginseng wine:

[0080] Accurately draw 5ml of the wine sample into a 100ml porcelain evaporating dish, evaporate to dryness in a boiling water bath, volatilize to remove ethanol, transfer it to a 50ml volumetric flask with distilled water, and dissolve it to the mark, shake well, filter with dry filter paper, and accurately absorb 1ml of the filtrate On the column, after the sample is on the column, first wash the impurities with 50ml distilled water, then wash the impurities with 25ml 20% (V / V) ethanol aqueous solution, after washing away the impurities, use 25ml 70% (V / V) ethanol to elute the adsorbed on For the total ginsenosides on the column, use a 100ml porcelain evaporating dish to receive the eluent, and evaporate to dryness in a boiling water bath. At the same time, accurately draw 1ml of ginsenoside Re standard product sold by China Pharmaceutical and Biological Products Inspection Institute, according to the above-menti...

Embodiment 2

[0085] Determination of total ginsenosides in a non-alcoholic oral liquid containing ginseng:

[0086] First filter the sample with dry filter paper, accurately draw 5ml of the filtrate into a 100ml volumetric flask, add distilled water to dissolve to the mark, shake well, accurately absorb 1.5ml of the diluted filtrate and put it on the column, first wash the impurities with 40ml of distilled water, and then use 25ml of 30 % (V / V) ethanol aqueous solution washing impurity, after washing off impurity, with 25ml 80% (V / V) ethanol aqueous solution eluting the total ginseng saponins adsorbed on the post, receive eluent with 100ml porcelain evaporating dish, in Evaporate to dryness in a boiling water bath. At the same time, accurately absorb 1ml of 150 μg / ml ginsenoside Re standard sold by China Institute for the Control of Pharmaceutical and Biological Products in the same manner as the above-mentioned sample loading, washing, elution, collection, and evaporation to dryness. Acc...

Embodiment 3

[0091] Determination of total ginsenosides in ginseng root:

[0092]First use a pulverizer to pulverize it through an 80-mesh sieve. It is known that this type of product generally contains about 3000-5000mg / 100g of total ginsenosides. Therefore, accurately weigh 0.5g of the pulverized powder that passed the 80-mesh, and put it in a 100ml volumetric flask. Add about 50ml of distilled water, ultrasonically extract for 20min, put it at room temperature, and fix the solution to the mark, shake well, filter with dry filter paper, accurately draw 1ml of the filtrate onto the column, first wash the impurities with 45ml of distilled water, and then use 30ml of 20% (V / V) Ethanol aqueous washing impurity, after washing away impurity, with 30ml70% (V / V) ethanol washing water desorption total ginsenosides on the adsorption column, receive eluent with 100ml porcelain evaporating dish, evaporate to dryness in boiling water bath. At the same time, accurately absorb 200 μg / ml (g / V) 1ml of th...

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Abstract

The invention provides a method for measuring general ginsenoside by an anthrone colorimetric method. The method comprises the following steps: extracting, propping, washing and eluting a sample; adding an anthrone visualization reagent to the sample and a standard product for development; measuring absorbency under a spectral photometer; and obtaining the general ginsenoside content of the sampleby calculation. The method has the advantages of high measuring speed, stable development, good repetitiveness, high accuracy, little relative error, and the like and is widely applicable to the measurement of various ginseng products.

Description

【Technical field】 [0001] The invention belongs to the technical field of total ginsenosides quantification, and more specifically, the invention relates to a method for determining total ginsenosides by an anthrone colorimetric method. 【technical background】 [0002] Ginsenosides are the main active ingredients in ginseng and American ginseng, and are the main raw materials for food, health products, and medicines. For the quality control of these products, the content of total ginseng saponins is a must-test indicator. Ginseng and American ginseng are Araliaceae plants. As early as the Eastern Han Dynasty, "Shen Nong's Materia Medica" listed ginseng as top grade. It has a history of 1,600 years. American ginseng has been cultivated and utilized in my country for more than 200 years in the 18th century. According to the 2005 edition of "Chinese Pharmacopoeia", they can treat physical weakness, cold extremities and weak pulse, cough due to lung deficiency, thirsty fluid wound...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28G01N1/34
Inventor 张连龙周华生成恒嵩
Owner WUXI JIANTE PHARM CO LTD
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