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Method for synchronously performing parallel detection of various biomarkers and chip test paper

A technology of biomarkers and markers, applied in biological testing, material inspection products, fluorescence/phosphorescence, etc., can solve the problems of long detection time, rapid detection cannot be quantified, etc., and achieve the effect of easy portability and easy use

Inactive Publication Date: 2010-03-03
SHANGHAI LINC BIO SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for simultaneous detection of multiple biomarkers and chip test strips to change the existing results of long detection time, rapid detection cannot be quantified, and only one marker can be obtained in the same detection. problems, so that it can quantitatively detect multiple biomarkers at the same time, improve the sensitivity of detection, and save detection time

Method used

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  • Method for synchronously performing parallel detection of various biomarkers and chip test paper
  • Method for synchronously performing parallel detection of various biomarkers and chip test paper
  • Method for synchronously performing parallel detection of various biomarkers and chip test paper

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Experimental program
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Effect test

preparation example Construction

[0046] Preparation of the ligand Bn-labeled signal substance: the source and type of the ligand Bn are the same as the ligand An (n=1, 2, 3, 4...), and the labeled signal substance can be fluorescein fluorescein (fluorescein: FITC, Cy2 TM, Alexa TM 488, Cy3 TM, Alexa TM 546 conventional fluorescein), fluorescent protein (GFP, R-PE, R-PC), nano gold, silver, nano fluorescent microspheres, quantum dots, rare earth elements, radioactive elements, small molecule chemicals or pigments). The ligand Bn and the labeling signal substance can be connected together by corresponding physical, chemical or biological methods through their corresponding physical and chemical properties to make them exist stably.

[0047] Production of chip test paper: chip test paper can have various shapes according to its fluidic method, and its basic components include: sample injection hole, storage position of ligand Bn marker, ligand An micro-arrangement and support, sample flow driving force Device (...

specific Embodiment

[0053] The implementation mode of the chip test paper detector:

[0054] see Figure 4 As shown, the detector includes: laser 31, filter group 32, optical filter 33, dichroic mirror 38, mirror 35, first objective lens 34, second optical filter 36, second objective lens 37, diaphragm 40 And chip test paper 39, and PMT (photomultiplier tube) components, AMP (signal amplifier) ​​components, converter A / D (signal data converter) components, CD chip, output printing equipment, main board and power supply equipment (not shown in the figure ).

[0055] Detector assembly and testing

[0056] According to the design diagram, connect the optical components in the instrument box in turn, and distribute the power supply and control board, and check the working conditions of each section of lines and components in turn to check whether they meet the requirements. After fully conforming, turn on the main board control program and power supply, and test the working condition of the system...

Embodiment approach

[0061] The material of the chip test paper can be NC film or glass sheet or plastic sheet, etc. The chip dot matrix is ​​5X6 or other arrangements (determined according to the specific product), the spacing is 1mm, the spot diameter is 10um-500um, and the overall width of the test paper is 3-30mm. Length range: 30-100mm. Appearance includes sampling hole, chip irradiation window, sealing card, positioning mark, etc. The internal structure of the chip test paper includes: filter paper, glass fiber where the ligand of the signal marker is located, and the ligand matrix (including test points, calibration points and quality control points), etc.

[0062] According to the above design and production test, the chip test paper detector fully meets its advantages of fast, sensitive, quantitative detection, multiple joint inspections, and easy to carry. It is a product with a wide range of applications in the future, which will play a role in promoting the development of clinical tes...

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Abstract

The invention relates to a method for synchronously performing parallel detection of various biomarkers and a piece of chip test paper. The method comprises the following steps that: a corresponding ligand An is fixed on a fibrous membrane or other fixed solid holders; the various biomarkers n and a ligand Bn form an affinity binding; and simultaneously the biomarkers move on the holders to combine with at least one ligand with a maker so as to form the ligand An, the biomarkers n, the ligand Bn and a signal marker. A conversion step and a calculation step of release signals comprise the following steps that: through the synchronous excitation of various markers on a piece of detected test paper, the markers generate energy jump after the excitation by exciting light and simultaneously release variant light waves or electronic substances; a detecting instrument receives signals of the light waves or electronic transmitters released by the markers and converts the signals into digital signals; and a data processing system calculates the signal data released by the markers and finally outputs the concentration of the biomarkers. The method can synchronously, quantitatively and quickly detect various biomarkers in one sample, shorten the detection time, and improve the sensitivity and the accuracy.

Description

technical field [0001] The invention relates to the technical field of in vitro immunoassay, in particular to a method for simultaneous detection of multiple biomarkers and a chip test paper. Background technique [0002] Since the invention of the enzyme-labeled detection technology platform in the 1970s, the immunodiagnosis industry has undergone earth-shaking changes, and the field of immunodiagnosis has grown rapidly. However, due to the long detection time of the enzyme-labeled detection system, the cumbersome operation of the intermediate process, the narrow detection range of the reagents, and the low sensitivity, the system has gradually withdrawn from the historical stage of clinical detection. Subsequently, colloidal gold standard technology platform, chemiluminescence, time-resolved fluorescence detection technology platform and immune chip technology platform were invented one after another. Colloidal gold label technology has played an important role in the fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/64
Inventor 罗朝领茅柳娟
Owner SHANGHAI LINC BIO SCI
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