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Traditional Chinese medicine composition containing isatis leaf and application thereof

The technology of a composition and Daqingye is applied to a traditional Chinese medicine composition containing Daqingye and its application field, which can solve the problems of inconvenience in application, difficult to meet large-scale promotion, etc., and achieves abundant drug sources and elimination of epidemic heat toxins. , the effect of suppressing pneumonia

Active Publication Date: 2011-04-27
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, traditional Chinese medicine needs syndrome differentiation and treatment before it can be prescribed. It is not very convenient to apply, and it is difficult to meet the requirements of large-scale promotion.

Method used

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  • Traditional Chinese medicine composition containing isatis leaf and application thereof
  • Traditional Chinese medicine composition containing isatis leaf and application thereof
  • Traditional Chinese medicine composition containing isatis leaf and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of anti-sensitivity spray

[0029] 1. Prescription:

[0030] Folium 150g

[0031] Dandelion 150g

[0032] Wild chrysanthemum 150g

[0033] Herb Herb 100g

[0034] Mint 50g

[0035] 2. Extraction process of water-soluble active ingredients

[0036] In the experiment, the amount of water added, the decoction time, and the number of times of decoction were taken as three factors when the Folium Folium Folium, wild chrysanthemum, and dandelion were mixed and decocted. Each factor was set at three levels, and the content of indirubin in the decoction was measured by HPLC. , with indirubin standard as the reference standard. Select with L 9 (3 4 ) Orthogonal design experiment, see Table 1.

[0037] Table 1: Table of Water Extraction Factor Levels

[0038]

[0039] Chromatographic conditions and system suitability test: with C 18 (250mm×4.6mm, 5μm) as filler; methanol-0.1% phosphoric acid (72:28) as mobile phase, flow rate: 1ml / min; ...

Embodiment 2

[0053] Example 2: Research on the in vitro effect of the anti-infection pharmaceutical composition containing Folium Folium on influenza virus

[0054] 1. Materials and methods

[0055] (1) Preparation of virus strains Take PR8 influenza virus strain (A / PR / 8 / 34, provided by Zhejiang Provincial Center for Disease Control and Prevention), inoculate the allantoic cavity of 9-11-year-old SPF chicken embryos, harvest after 48 hours, and make blood Coagulation titer titration, aliquot into small tubes, and store at -80°C. Take one tube for 10-fold gradient dilution, inoculate 5 chick embryos of the right age in each group with the virus solution of each concentration, incubate at 34°C for 72 hours, take the chicken embryo allantoic fluid to titrate the hemagglutination (HA) titer, and calculate the half infection dose (ID50 ).

[0056] (2) Determination of drug toxicity to chicken embryos Adjust the anti-infection spray (prepared according to Example 1) to a concentration of 0.12 ...

Embodiment 3

[0070] Example 3: Effect of anti-infection pharmaceutical composition containing Folium Folium on lung index of mice infected with influenza virus

[0071] 1. Purpose of the test

[0072] To observe the effect of anti-influenza pharmaceutical composition containing Folium Folium on the lung index of influenza virus infection in ICR mice, and compare it with the positive control product—Antiviral Soft Capsules, so as to provide a preliminary basis for evaluating the research and development of anti-influenza spray.

[0073] 2. Test drugs

[0074] (1) Test sample

[0075] Anti-sensitivity spray: prepared according to the method of Example 1, brown viscous liquid (containing 1.2g of crude drug per ml), stored in a dry place at 4°C, and prepared to the required concentration with physiological saline before use.

[0076] (2) Positive control substance

[0077] Antiviral Soft Capsule: This product is a soft capsule, the content is a dark brown viscous liquid, with a slightly fra...

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Abstract

The invention provides a traditional Chinese medicine composition containing isatis leaf, which comprises the following components in parts by mass (proportioning according to the dry weight respectively): 10-20 parts of isatis leaf, 10-20 parts of dandelion, 10-20 parts of wild chrysanthemum flower, 5-20 parts of prepared rhizoma atractylodis and 1-10 parts of peppermint. The traditional Chinesemedicine composition containing isatis leaf mainly has the following advantages: (1) the traditional Chinese medicine composition has reasonable formula, has the function of clearing away heat and toxic substances and can effectively remove epidemic toxic heat; (2) the cold-preventing medicine composition provided by the invention has low cost and abundant medicine source; (3) the traditional Chinese medicine composition has a certain suppressing function on influenza A virus strains in vitro; and (4) the traditional Chinese medicine composition can effectively suppress the generation and thedevelopment of pneumonia caused by the infection of influenza viruses.

Description

(1) Technical field [0001] The invention relates to a traditional Chinese medicine composition containing Folium Folium, its application in preparing medicines for preventing and treating influenza virus, and a spray for preventing and treating influenza prepared by using the traditional Chinese medicine composition. (2) Background technology [0002] Influenza is a respiratory infectious disease that seriously endangers human health. Because influenza virus is easy to mutate and form new strains, it brings great difficulties to vaccine prevention. Therefore, influenza is a very important viral infectious disease that cannot be completely controlled so far. , and is also the first infectious disease in the world to achieve global surveillance. Western medicine still has no targeted effective method and medicine at present. The application of Chinese herbal medicine to treat influenza has a history of thousands of years, and a wealth of theoretical and practical experience h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/534A61K9/12A61P31/16
Inventor 徐珊李范珠张永生陈民利吴巧凤朱飞叶
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY