Synthetic human insulin gene and use thereof in cultivation of transgenic tomatoes

Abstract

The invention relates to the field of plant gene engineering and provides a sequence of a human insulin gene designed and synthesized according to plant preferred codons. A plant expression vector isbuilt for the synthetic human insulin gene, so that the gene can be transferred into tomatoes by an agrobacterium rhizogenes-mediated method under the drive of a CaMV 35S promoter and a fruit-specificpromoter 2A12 to express human insulin in tomato fruit. The tomatoes capable of making human insulin can be used as a convenient oral product for preventing and treating insulin-dependent diabetes mellitus and autoimmune disorder diabetes mellitus, and human insulin can be extracted from the tomatoes to be made into injection preparations.

Description

[0001] This application is a divisional application of CN03109739.1. The original application title is: Method for Producing Human Insulin Using Transgenic Tomato. The filing date of the original application is: April 14, 2003. Technical field: [0002] The invention relates to a method for producing human insulin by transgenic plants and its product. Background technique: [0003] Bioreactors are an area of ​​great concern to many biotechnology R&D departments in the near future. Human growth factors are produced in mammary bioreactors such as dairy cows and dairy goats. In the research of plant bioreactor, the research of using potato tubers to produce hepatitis B vaccine has received extensive attention. Lettuce and tomato, as vegetables or fruits that can be eaten raw, have great application potential in the production of oral polypeptide drugs. Human insulin is very important for the treatment of insulin-dependent diabetic patients. Metabolic regulation in some diabe...

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Problems solved by technology

At the same time, the dendritic cells scattered in the intestinal cells can also take up the antigens in the intestinal lumen and activate the immune response through the circulation of the lymphatic system, but whether this process induces tolerance or immunity is still unclear

[0007] The treatment of oral tolerance has a certain therapeutic effect on the disease because it can induce antigen-specific immune responses, but it is limited by some practical problems, namely This therapy requires large amounts of protein antigens in both humans and experimental animals

To address this issue, several traditional expression systems, including microbial fermentation and mammalian cells, have been experimented with, however, they all have methodological limitations.

A major problem with bacterial expression systems is their inability to perform the post-translational processing necessary for many mammalian proteins, such as glycosylation

While the mammalian cell expression system overcomes this problem, its expensive culture equipment and its relatively low yield make it expensive and impractical

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