Oligopeptide phase reversal chromatography stationary phase and preparation method thereof

A technology of reversed-phase chromatography and stationary phase, applied in chemical instruments and methods, and other chemical processes, can solve problems such as scarcity, and achieve the effect of single reaction site, high selectivity, high conversion rate, and regular and orderly structure
CN101690887AInactive Publication Date: 2010-04-07EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2010-04-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an oligopeptide phase reversal chromatography stationary phase and a preparation method thereof. The preparation method adopts 'link chemistry' as a bonding reaction method to bond oligopeptide molecules and comprises the following steps: firstly, introducing terminal alkynyl on a silica gel surface; and then, taking methanol, acetonitrile, dimethyl sulfoxide or a mixture of the solvents as a reaction solvent to bond oligopeptide modified with azide groups on the silica gel surface, thereby obtaining the oligopeptide phase reversal chromatography stationary phase. The oligopeptide phase reversal chromatography stationary phase has orderly structure and single reaction site; moreover, the structure of the stationary phase introduces triazole rings, urea bonds, benzene rings, peptide bonds and ester groups to generate multiple kinds of acting force, such as hydrogen-bonding action, electrostatic interaction, Pi-Pi action, dipole-dipole interaction, hydrophobic interaction and the like, thereby realizing orthogonal separation on complex compositions in a two-dimensional structure constructed with C18; and 'link chemistry' is adopted as the bonding reaction method to realize immobilization of high selectivity and high conversation efficiency under moderate conditions.
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Description

【Technical field】

[0001] The invention relates to liquid chromatography stationary phase technology, specifically, a method for preparing a reversed-phase chromatography stationary phase using "click chemistry" as a bonding method to bond oligopeptides. 【technical background】

[0002] Reversed-phase high-performance liquid chromatography (RP-HPLC for short) is currently the most widely used high-efficiency separation technology. However, the most commonly used hydrophobic stationary phases in reversed-phase chromatography, such as C18, C8, etc., are mainly long-chain alkanes, resulting in The force between the stationary phase and the separated components is too single (mainly hydrophobic interaction), which makes the separation of complex components (such as in the fields of proteomics, metabolomics, pharmaceutical industry, natural products, etc.) huge challenge. Reversed-phase / reversed-phase two-dimensional chromatography is an important means for the separation of compl...

Claims

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