Specific purified high-molecular urokinase chromatography media as well as preparation method and application thereof

Abstract

A specific purified high-molecular urokinase chromatography media is characterized by having a structure as follows: HOOC-(CH2)4(NH)C-O-R-O-C(NH)NH(CH2)4-CO-X,X=NHC6H5C(NH)NH2, wherein R represents cross-linked agarose substrate. The specific purified high-molecular urokinase chromatography media has the advantages of being simple in preparation process and mild for reaction conditions, being applicable to high-efficiency separation of high-molecular urokinase media and low-molecular urokinase media, and being capable of implementing industrial production of high-purification and high-molecular urokinase.

Description

technical field

[0001] The invention relates to a high molecular urokinase chromatographic medium and a preparation method, in particular to a specific purification high molecular urokinase chromatographic medium, a preparation method and an application. Background technique

[0002] According to the forecast of Datamonitor, a market research organization, by 2015, the total market value created by global novel antithrombotic drugs will reach about 10 billion US dollars. Among them, the thrombolytic agent plasminogen activators (PAs) class of drugs reached 1.5 billion US dollars. The main plasminogen activators include streptokinase (Streptokinase), urokinase (Urokinase), tissue plasminogen activator (tPA) and so on. With the aging of the world's population, the demand for such drugs will inevitably increase further. As one of the thrombolytic drugs, urokinase can directly promote the inactive plasminogen into active plasmin, and hydrolyze the fibrin that makes up the thro...