Device and method for preparing gel microspheres with uniform grain sizes

A technology for gel microspheres and preparation devices, which is applied in the preparation of microspheres and microcapsule preparations. It can solve the problems of adverse effects on the activity of embedding objects, difficulty in meeting actual needs, and high cost of preparation processes, and reduce the application of external forces. Equipment requirements, good permeability, and the effect of stable preparation process

Inactive Publication Date: 2010-04-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microspheres prepared by the emulsification method and the coaxial airflow method have poor particle size uniformity, which is difficult to meet the actual needs; the extrusion method is difficult to prepare microspheres with small particle sizes; Because it adopts micron-scale pipelines that are thinner than the diameter of microspheres, the preparation process is easy to block; the membrane emulsification method has problems such as high equipment requirements and high preparation process co

Method used

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  • Device and method for preparing gel microspheres with uniform grain sizes
  • Device and method for preparing gel microspheres with uniform grain sizes
  • Device and method for preparing gel microspheres with uniform grain sizes

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preparation example Construction

[0017] The specific preparation method of the microspheres includes the following steps: connect the container containing the oil phase to the inlet port of the constant flow pump 5 and set the flow rate of the constant flow pump, the oil phase passes through the hose 6 into the support tube 3 and flows into the capillary glass In the tube 1; the dispersed phase is loaded into the syringe of the micro-injection pump 4 and the flow rate of the micro-injection pump 4 is set, and the dispersed phase is passed through the flexible pipe 6 into the stainless steel needle 2 and flows into the capillary glass tube 1; the two-phase liquid flow is in the After the capillary glass tubes meet, the dispersed phase is pulled into a thin stream by the oil phase, and the end of the thin stream breaks periodically to form droplets with uniform particle size, and the oil containing the dispersed phase droplets is collected at the end of the capillary glass tube 1 with a glass container 7 phase, ...

Embodiment 1

[0023] Microsphere preparation device among the present invention: select the capillary glass tube of length 30cm (inner diameter 940 micron, outer diameter 1250 micron) and the needle head of 8cm (inner diameter 270 micron, outer diameter 600 micron).

[0024] The outlet ends of the micro injection pump 4 and the constant flow pump 5 are respectively connected to the stainless steel needle 2 and the bracket tube 3 through flexible tubes, and the bracket in the bracket tube 3 inserts the stainless steel needle 2 and fixes it on the central axis of the capillary glass tube 1 , and make the support tube 3 communicate with the capillary glass tube 1, and the oil phase containing the dispersed phase droplets with uniform particle size flowing out from the end of the capillary glass tube 1 is collected by the glass container 7.

Embodiment 2

[0026] Using the natural polysaccharide sodium alginate as the material of the dispersed phase and paraffin oil as the oil phase, the preparation process of the microspheres is described in detail by using the device prepared in Example 1.

[0027] Sodium alginate with a viscosity of 125cps was used to prepare a sodium alginate aqueous solution with a concentration of 4% as the dispersed phase; paraffin oil was used as the oil phase, and Span 80 with a concentration of 1% was added to the paraffin oil as an emulsifier.

[0028]Connect the container containing 1% Span 80 paraffin oil to the inlet of the constant flow pump 5, set the flow rate of the constant flow pump to 70rpm, pass the oil phase through the hose 6 into the support pipe 3 and flow into the capillary glass Tube 1: Put the sodium alginate solution as the dispersed phase into the syringe of the micro-injection pump 4, set the flow rate of the micro-injection pump 4 to 0.3ml / h, pass the dispersed phase into the stai...

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Abstract

The invention relates to a device and a method for preparing gel microspheres with uniform grain sizes. The outlet ends of a micro-dosage syringe pump and a constant-flow pump of the device are connected with a syringe needle and a stand tube through a hose respectively, a stand in the stand tube inserts and fixes the syringe needle at the position of a center shaft of a capillary glass tube, and the stand tube is communicated with the capillary glass tube. The method comprises the following steps: connecting a container with an oil phase into the inlet end of the constant-flow pump, setting the flow rate of the constant-flow pump, and introducing the oil phase into the stand tube through the hose to ensure that the oil phase flows into the capillary glass tube; filling dispersed phases into a syringe tube of the micro-dosage syringe pump, setting the flow rate of the pump, and introducing the dispersed phases into the syringe needle to ensure that the dispersed phases flow into the capillary glass tube; and collecting the oil phase containing dispersed phase liquid drops by using a glass container at the tail end of the capillary glass tube, solidifying dispersed phase droplets, and washing the mixture to remove the oil phase so as to obtain the gel microspheres with uniform grain sizes. The device and the method have a simple process and can prepare the microspheres with the grain sizes between 10 and 50 microns, and the prepared gel microspheres can be widely used for embedding enzymes, cells or microorganisms and the like.

Description

technical field [0001] The invention relates to a device and method for embedding cells, microorganisms and enzymes, and for sustained release of drugs to encapsulate drugs, in particular to a device and method for preparing gel microspheres with uniform particle diameters. Background technique [0002] There are microspheres with uniform particle size for embedding enzymes, cells, microorganisms, etc., which have advantages in signal detection, tracking, and simulation of microspheres, and microspheres of similar size can provide similar microenvironments for embedding objects. The outside world produces a similar reaction, which is convenient for studying the effect of embedding. Due to these characteristics of the microspheres with uniform particle size, they are widely used in many fields such as biology, medicine and chemical industry. For different materials and different applications, the preparation methods involved are also different. When microspheres are used fo...

Claims

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Application Information

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IPC IPC(8): B01J13/02
Inventor 刘晨光冀世奇刁瑞玉张晓华
Owner OCEAN UNIV OF CHINA
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