Device and method for preparing gel microspheres with uniform grain sizes

Abstract

The invention relates to a device and a method for preparing gel microspheres with uniform grain sizes. The outlet ends of a micro-dosage syringe pump and a constant-flow pump of the device are connected with a syringe needle and a stand tube through a hose respectively, a stand in the stand tube inserts and fixes the syringe needle at the position of a center shaft of a capillary glass tube, and the stand tube is communicated with the capillary glass tube. The method comprises the following steps: connecting a container with an oil phase into the inlet end of the constant-flow pump, setting the flow rate of the constant-flow pump, and introducing the oil phase into the stand tube through the hose to ensure that the oil phase flows into the capillary glass tube; filling dispersed phases into a syringe tube of the micro-dosage syringe pump, setting the flow rate of the pump, and introducing the dispersed phases into the syringe needle to ensure that the dispersed phases flow into the capillary glass tube; and collecting the oil phase containing dispersed phase liquid drops by using a glass container at the tail end of the capillary glass tube, solidifying dispersed phase droplets, and washing the mixture to remove the oil phase so as to obtain the gel microspheres with uniform grain sizes. The device and the method have a simple process and can prepare the microspheres with the grain sizes between 10 and 50 microns, and the prepared gel microspheres can be widely used for embedding enzymes, cells or microorganisms and the like.

Description

technical field [0001] The invention relates to a device and method for embedding cells, microorganisms and enzymes, and for sustained release of drugs to encapsulate drugs, in particular to a device and method for preparing gel microspheres with uniform particle diameters. Background technique [0002] There are microspheres with uniform particle size for embedding enzymes, cells, microorganisms, etc., which have advantages in signal detection, tracking, and simulation of microspheres, and microspheres of similar size can provide similar microenvironments for embedding objects. The outside world produces a similar reaction, which is convenient for studying the effect of embedding. Due to these characteristics of the microspheres with uniform particle size, they are widely used in many fields such as biology, medicine and chemical industry. For different materials and different applications, the preparation methods involved are also different. When microspheres are used fo...

AI Technical Summary

Problems solved by technology

The microspheres prepared by the emulsification method and the coaxial airflow method have poor particle size uniformity, which is difficult to meet the actual needs; the extrusion method is difficult to prepare microspheres with small particle sizes; Because it adopts micron-scale pipelines that are thinner than the diameter of microspheres, the preparation process is easy to block; the membrane emulsification method has problems such as high equipment requirements and high preparation process costs; For objects such as cells and microorganisms, the preparation method involved must not only meet the actual requirements for particle size, but also must consider the influence of the preparation conditions of the microspheres on the activity of the embedding objects.

Microsphere methods such as spray drying and chemical crosslinking are not suitable for such applications, and some severe shear forces used in some emulsification methods can adversely affect the activity of the embedding object

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