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Method for extracting polysaccharose from ginseng and application

A technology of ginseng and polysaccharide, applied in the field of medicine, can solve the problem of preventing or treating osteoarthritis without using ginseng polysaccharide, and achieves the effect of strong administration effect, wide source and small toxic and side effects

Inactive Publication Date: 2010-04-28
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of ginseng alone in the treatment of osteoarthritis, and there is no report on the use of polysaccharides extracted from ginseng to prevent or treat osteoarthritis.

Method used

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  • Method for extracting polysaccharose from ginseng and application
  • Method for extracting polysaccharose from ginseng and application
  • Method for extracting polysaccharose from ginseng and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The method for extracting ginseng polysaccharide from ginseng, the steps are:

[0049] A, the dried ginseng is powdered into 80 or 85 or 90 or 95 or 100 mesh powder with pulverizer;

[0050] B. Put the powder into the extraction tank, add 3 times (w / v, g / mL) of petroleum ether (boiling range: 60°C to 90°C) and reflux for 60 minutes, degrease, repeat this step twice, and filter out the petroleum ether , to obtain the residue after degreasing;

[0051] C. Add 7 times (w / v, g / mL) distilled water to the residue to extract at 100°C for 2 hours, repeat this step three times, and combine the three extracts;

[0052] D, 50 ℃ decompression (1 × 10 4 pa) concentrating the extract so that the volume of the solution is 5 times the weight (g) of the ginseng powder used in step A to obtain a concentrate;

[0053] E. Add 95% (v / v) ethanol of 3 times the volume of the concentrated solution to the concentrated solution in step D, and obtain ginseng polysaccharide precipitation after ...

Embodiment 2

[0058] The method for extracting ginseng polysaccharide from ginseng, the steps are:

[0059] A, the dried ginseng is crushed and passed through 80 or 85 or 90 or 95 or 100 mesh sieves;

[0060] B. Put it into an extraction tank, add petroleum ether (boiling range is 60°C to 90°C) with a liquid-to-solid ratio (w / v, g / mL) of 5:1, and reflux twice for 45 minutes each time to degrease. Obtain a degreasing residue;

[0061] C. Add 11 times (w / v, g / mL) distilled water to the residue to extract three times at 100°C, each time for 1.5 hours, and combine the extracts;

[0062] D, 65 ℃ decompression (5 × 10 4 pa) concentrating the extract so that the solution volume (mL) is 6 times the weight (g) of the bulk drug powder used in step A to obtain a concentrated solution;

[0063] E. Add 95% (v / v) ethanol of 3 times the volume of the concentrated solution to the concentrated solution in step D, and obtain ginseng polysaccharide precipitation after standing overnight;

[0064] F, centr...

Embodiment 3

[0068] The method for extracting ginseng polysaccharide from ginseng, the steps are:

[0069] A, dry ginseng is pulverized into 80 or 85 or 90 or 95 or 100 mesh powder;

[0070] B. Add petroleum ether (boiling range: 60° C. to 90° C.) in an amount 7 times (w / v, g / mL) of raw medicinal materials and reflux for 30 minutes to degrease twice and then filter to obtain a degreased residue;

[0071] C. Add distilled water to the residue obtained in step B at a liquid-to-material ratio (w / v, g / mL) of 15:1, extract at 100°C for 1 hour, repeat this step three times, and combine the extracts;

[0072] D, depressurize the extract obtained in step C at 80°C (9×10 4 pa) concentrating so that the solution volume (mL) is 7 times the weight (g) of the ginseng powder obtained in step A to obtain a concentrated solution;

[0073] E. Add 3 times the volume of 95% (v / v) ethanol to the concentrated solution of step D, and obtain ginseng polysaccharide precipitation after standing overnight;

[00...

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Abstract

The invention discloses a method for extracting polysaccharose from ginseng and the application; the method comprises the following steps: a. the dried ginseng is smashed; b. the smashed ginseng is placed in an extract pot, petroleum ether is added to carry out refluxing and degreasing, the petroleum ether is filtered out, and degreased residue is obtained; c. distilled water is added in the residue for extraction at normal pressure, boiling extraction is repeatedly carried out for three times, and the extracting solution for three time is mixed; d. pressure reduction concentration is carried out to the extracting solution, so as to lead the volume of the solution to be equal to the volume of the ginseng powder in the step a and obtain the concentrated solution; e. the concentrated solution is added in the concentrated solution in the step d, the mixture is placed still for a night and then is settled; f. the mixture is centrifuged to obtain the sediment which is died, and then the dried sediment is washed respectively by absolute alcohol, acetone and ether after being deproteinized with a Sevag method; g. the sediment is dialyzed in water by a semipermeable membrane with 1000-10000 of cut-off molecular weight, and then is frozen and dried to obtain ginseng polysaccharide. The invention further relates to the application of the ginseng polysaccharide for preparing osteoarthritis drugs which are used for treating or preventing the osteoarthritis; in the invention, effective constituents are clear, the healing efficacy is ensured without side and toxic effect, and the extraction method is easy to operate.

Description

technical field [0001] The invention relates to the technical field of medicine, more specifically to a method for extracting ginseng polysaccharide from ginseng, and also relates to the use of the ginseng polysaccharide extracted from ginseng in preventing and treating osteoarthritis. Background technique [0002] Articular cartilage tissue is mainly composed of chondrocytes and extracellular matrix (ECM), and the main components of ECM are type II collagen, proteoglycan aggregates and water. The degradation and repair of normal articular cartilage matrix are in a continuous dynamic equilibrium state, which is maintained by the secretion of cartilage matrix components by chondrocytes. Osteoarthritis is a chronic joint disease characterized by focal articular cartilage degeneration, bone loss, joint edge osteophyte formation, joint deformity and subchondral bone density (sclerosis). Osteoarthritis. With the advent of an aging society and the extension of the average human ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P19/02
Inventor 汪晖陈廖斌谭扬胡爱心陈彪刘军
Owner WUHAN UNIV
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