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Detection method of persimmon leaf quality

A detection method and persimmon leaf technology, applied in the field of persimmon leaf quality detection, can solve the problems of unstable persimmon leaf quality, lack of standardization of persimmon leaves, inability to effectively detect and control the quality of persimmon leaves in appearance and taste, and the like. Accurately determined effect

Active Publication Date: 2010-05-05
GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The prior art mainly embodies the main properties and characteristics of persimmon leaves when they are used in medicine, usage and dosage, storage methods, etc., but the quality of persimmon leaves cannot be effectively detected and controlled only by appearance, property and taste, and in the existing There is no practical and effective method for the quality control of persimmon leaves in the technology, which leads to the instability of the quality of persimmon leaves and the lack of standardization of persimmon leaves in medicinal use

Method used

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  • Detection method of persimmon leaf quality
  • Detection method of persimmon leaf quality
  • Detection method of persimmon leaf quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Because persimmon leaves mainly contain flavonoids, which are also the main active ingredients in persimmon leaves, and the flavonoids in persimmon leaves are mainly quercetin and kaempferol aglycones and their glycosides. Therefore, quercetin and kaempferol can be identified by conventional methods of flavonoids.

[0052] The specific steps are:

[0053] Take persimmon leaves to be side product powder 1g and fully mix with methanol 25ml, the mixture is ultrasonically treated for 30 minutes and then filtered, and the filtrate is concentrated to 1ml as the test solution.

[0054] Another 1 g of persimmon leaves as reference medicinal material was taken, and the same method was used to prepare a reference medicinal material solution.

[0055] Then take kaempferol and quercetin reference substances, mix them with methanol to make a mixed solution containing 0.1 mg per 1 ml, and use the mixed solution as the reference substance solution.

[0056] Test according to thin la...

Embodiment 2

[0059] Flavonoids are the main active ingredients in persimmon leaves, and the flavonoids in persimmon leaves are mainly quercetin and kaempferol aglycones and their glycosides. The total amount of aglycone in quercetin and kaempferol after hydrolysis of persimmon leaves can basically reflect the total flavonoid content in persimmon leaves, and the total flavonoid content can reflect the content of the active ingredients in persimmon leaves——persimmon leaves. Therefore, the present invention also includes a method for determining the content of persimmon leaves in the persimmon leaves test product by using high performance liquid chromatography. Specific steps are as follows:

[0060] Use octadecylsilane bonded silica gel as the filler, methanol-0.2% phosphoric acid mixed solution (50:50) as the mobile phase, the detection wavelength is 360nm, and the number of theoretical plates should not be less than 2500 based on the quercetin peak .

[0061] Take kaempferol and querceti...

Embodiment 3

[0067] In addition to the two testing methods of TLC identification and HPLC determination of the content of persimmon leaf in the persimmon leaf test product, the present invention can also include the identification of persimmon leaf powder.

[0068] The characteristics of persimmon leaf powder are: brownish yellow powder. Epidermal cells are polygonal or irregular. With many non-glandular hairs, unicellular, hooked or S-shaped curved. The pores are indeterminate pores. The catheter is a threaded or stepped catheter, and some catheters contain brown lumps. The glandular hairs are multicellular and the head is brownish-yellow. The square crystals of calcium oxalate in the mesophyll are large and numerous, and sometimes the crystal cells are connected, and the square crystals are arranged in rows. There are many palisade organizations.

[0069] Grind the persimmon leaves to be tested, add methanol and mix thoroughly, add developing agent to the mixture to develop, and the...

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Abstract

The invention provides a detection method of persimmon leaf quality; which is characterized in that: the method comprises the following steps: sample solution preparation: the persimmon leaf powder to be detected is taken out and mixed with methyl alcohol to obtain a first mixed solution which carries out ultrasonic wave treatment to filter the first mixed solution, and the obtained filtrate is concentrated to obtain test article solution; kaempferide, quercetin and methanol are mixed to obtain a second mixed solution which is used as reference substance solution; detection: the sample solution is measured by thin layer chromatography, the chromatograph of the test article is observed weather to display the spots with same color with the reference substance at the same position of the chromatograph of the reference substance solution. If yes, the persimmon leaf powder to be detected is determined to contain the kaempferide and the quercetin; compared with the method that the persimmon leaf quality is determined by the characteristics in the prior art, the detection method can accurately determine the drug effective ingredients of the persimmon leaf to be detected.

Description

technical field [0001] The invention relates to the field of medicine quality control, in particular to a method for detecting the quality of persimmon leaves. Background technique [0002] The use of persimmon leaves as medicine has been recorded in "Southern Yunnan Materia Medica" more than 600 years ago. Its good effects on relieving cough and asthma, lung account, internal bleeding, and lowering blood pressure make it an important position in the family of traditional Chinese medicine. In the prior art, after the persimmon leaves are extracted with water and alcohol-precipitated, the water extract is extracted with ethyl acetate to obtain the persimmon leaf extract, and then the traditional Chinese medicine preparation made of the persimmon leaf extract and auxiliary materials is Guangzhou Baiyunshan Hutchison Whampoa Traditional Chinese Medicine Co., Ltd.'s exclusive protection of traditional Chinese medicines is recorded on page 294 of the twentieth volume of tradition...

Claims

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Application Information

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IPC IPC(8): A61K36/44A61P11/06A61P11/14A61P11/00A61P7/04A61P9/12G01N30/90G01N30/02A61K127/00
Inventor 李楚源尹仁杰王德勤梁敬仪罗杰利幼张小雪林挺陈薇
Owner GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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