Production method of protease hydrolyzing leftovers of freshwater fish and other fish efficiently

A production method, protease technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of expensive products, achieve the effects of improving permeability, strong elastase activity, and promoting secretion

Active Publication Date: 2010-05-12
JIMEI UNIV
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the production method of elastase is extracted from pig pancreas, and the product

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method of protease hydrolyzing leftovers of freshwater fish and other fish efficiently

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Embodiment 1

[0017] The present invention is a protease production method that can efficiently hydrolyze freshwater fish and fish scraps. The process steps include: (1) strain activation: the culture medium for strain activation consists of 0.4% beef extract and 0.6% peptone. Yeast extract 0.2%, NaCl 0.5%, agar 2%, water content 96.3%, pH 7.5; the culture temperature for strain activation is 37°C, and the culture time is 24h; (2) Seed preparation: The composition of the medium for seed preparation is: Beef extract 0.4%, peptone 0.6%, yeast extract 0.2%, NaCl 0.5%, moisture 98.3%, pH 7.5; shake flask strain preparation is carried out in a 250ml Erlenmeyer flask, the seed culture medium is 20ml, and the seeds After the medium is prepared, inoculate the newly activated slant moss into the medium, put it in a shaker, and cultivate for 24 hours at 180r / m and 32°C; the preparation of workshop seeds is carried out in a seed tank. The loading factor of the medium is 0.7, the in...

Example Embodiment

[0018] Embodiment 2

[0019] This embodiment is basically the same as the first embodiment, the difference is: (3) Preparation of Bacillus subtilis culture medium, the composition of the culture medium is: glucose 8%, corn steep liquor 1%, K 2 HPO 3 0.2%, MgSO 4 ·7H 2 O0.034%, water content 90.77%; the loading coefficient of the medium is 0.7; after the medium is sterilized and cooled, the inoculation amount is 10%, and the bacteria are inserted at the fermentation temperature of 37℃ and dissolved oxygen of 30%±5% 、Cultivate for 16h under the condition of pH 7.4±0.2 (4) Add 0.1% inducer and 0.06% triton-100, raise the fermentation temperature to 40℃ and keep the dissolved oxygen and pH unchanged, continue the fermentation for 16h according to the process instructions Step (5) Extract elastase and prepare enzyme powder.

Example Embodiment

[0020] Embodiment 3

[0021] This embodiment is basically the same as the first embodiment, the difference is: (3) Preparation of Bacillus subtilis culture medium, the composition of the culture medium is: glucose 4.5%, corn steep liquor 0.6%, K 2 HPO 3 0.13%, MgSO 4 ·7H 2 O 0.034%, moisture 94.74%; the loading factor of the medium is 0.7; when the medium is sterilized and cooled, insert the newly cultivated seeds with 10% of the inoculum at the fermentation temperature of 37℃, dissolved oxygen 30% ± After culturing for 14 hours under the condition of 5% and pH 7.4±0.2, add 0.1% inducer and 0.06% triton-100, increase the fermentation temperature to 37℃ and maintain the dissolved oxygen and pH of the fermentation, continue the fermentation for 16 hours according to the process The instruction step (5) extracts elastase and prepares enzyme powder.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a production method of a novel protease. The protease produced by the method has stronger elastase activity and can hydrolyze leftovers of freshwater fish and other fish processing efficiently. The production method of the protease can be summarized as: culturing bacillus subtilis by culture medium without organic nitrogen source till logarithmic phase, adding collagen andtriton-100 extracted from fish scales or fish skin, raising fermentation temperature by 2-3 DEG C, and fermenting continuously for 16h so that elastase activity can reach 650U/mL and total protease activity can reach 2,216U/mL; extracting protease and preparing into powdery enzymic preparation with total protease activity being 18,000U/g and elastase activity being 5,423U/g. Hydrolysis efficiencycan be greatly enhanced by using the protease preparation to hydrolyze leftovers of freshwater fish and other fish processing.

Description

technical field [0001] The invention relates to a method for producing novel protease and its preparation by fermenting Bacillus subtilis, in particular to a protease production method capable of efficiently hydrolyzing freshwater fish and fish waste, and its preparation technology belongs to the technical field of fermentation engineering and biochemical industry , and its application technology involves the fields of medicine, food and cosmetics. Background technique [0002] Enzymatic hydrolysis of low-value fish and fish processing waste to produce high value-added products is an important way for low-value fish and fish comprehensive utilization. Freshwater fish and fish scraps are rich in collagen and less endogenous elastase, and the current commercial protease (alkaline protease, acid protease, compound protease, neutral protease, trypsin, papain, etc.) During hydrolysis, there are generally problems such as low hydrolysis yield and incomplete hydrolysis; therefore,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/66C12R1/125
Inventor 倪辉蔡慧农肖安风李利君杨远帆
Owner JIMEI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap