Artificial nerve graft of neurotrophic factor fixed by genipin and preparation method thereof

A technology of neurotrophic factors and artificial nerves, applied in the field of artificial nerve grafts and its preparation, to achieve nerve function recovery, good biocompatibility, and good use effects

Inactive Publication Date: 2010-05-19
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As for whether genipin reacts with amino acid biomacromolecules in the form of monomers or in the form of dimers or multimers, it is still inconclusive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Heat treatment of silk at 50-100°C in a weak alkali solution (0.1-10% weight concentration of sodium carbonate or 0.1-10% weight concentration of potassium carbonate), and then wash the treated fibers with distilled water to obtain silk fibroin fibers, Part of the silk fibroin fibers prepared above are dissolved in a ternary system of calcium chloride, ethanol and water at 25-80°C (eg 25°C, 50°C, 80°C) (the molar ratio is calcium chloride: Ethanol: water=1: 2: 8), dissolving time is 0.5~6 hours (example 0.5, 3, 6 hours) the mixed solution of dissolving is packed into cellulose film bag, obtains the silk fibroin containing 1-10% with distilled water dialysis Protein aqueous solution, add 10mM genipin solution and neurotrophic factor solution, the volume ratio of silk fibroin aqueous solution and genipin solution is 0.2: 1-1: 4 (for example 0.5: 1, 1: 1, 1: 2), nerve The concentration of trophic factors in the nerve graft scaffold material, genipin and neurotrophic factor...

Embodiment 2

[0019] Dissolve chitosan with weak acid, weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), the concentration of lactic acid is 0.5~15% (example 2%, 8%, 15%), the concentration of chitosan is 0.5-5 % (eg 0.5%, 2%, 5%). Add a certain amount of 10mM genipin solution and neurotrophic factor solution, the volume ratio of silk fibroin solution to genipin solution is (0.2: 1-1: 4, example 0.5: 1, 1: 1, 1: 2), The concentration of neurotrophic factors in the nerve graft scaffold material, genipin and neurotrophic factor mixture is 1μg / ml-800g / ml, and the types include nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), nerve One or more of trophic factor 3 (NT-3), basic fibroblast growth factor (bFGF), glial cell-derived neurotrophic factor (GDNF), etc., and then inject the solution into the mold, cross-link and dry to obtain Chitosan artificial nerve grafts containing neurotrophic factors.

Embodiment 3

[0021] Add a certain amount of 10mM genipin solution and neurotrophic factor solution to the collagen solution with a concentration of 0.5-5% (eg 0.5%, 2%, 5%), and the volume ratio of the silk fibroin aqueous solution to the genipin solution is the volume ratio From 0.2:1-1:4 (for example 0.5:1, 1:1, 1:2), the concentration of neurotrophic factor in nerve graft scaffold material, genipin and neurotrophic factor mixture is 1 μg / ml- 800g / ml, types include nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophic factor 3 (NT-3), basic fibroblast growth factor (bFGF), glial cell-derived neurotrophic factor (GDNF), etc., and then inject the solution into the mould, cross-link and dry to obtain a collagen artificial nerve graft containing neurotrophic factors.

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PUM

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Abstract

The invention discloses an artificial nerve graft of a neurotrophic factor fixed by genipin and a preparation method thereof. A product comprises a nerve graft scaffold material, wherein the neurotrophic factor which is cross-linked by the genipin is arranged on the nerve graft scaffold material. The preparation method comprises the following steps of: adding a genipin solution and a neurotrophic factor solution into the solution of the nerve graft scaffold material, then molding and processing the solution, solidifying and cross-linking to obtain the required artificial nerve graft. The product contains the neurotrophic factor, and all the used materials are degradable materials which have favorable biologic compatibility with a human body. The prepared product does not contain external toxic or side effect substances carried in because of the preparation process.

Description

Technical field: [0001] The invention relates to an artificial nerve graft and a preparation method thereof. Background technique: [0002] In the real world, accidents such as traffic accidents, industrial accidents, sports accidents, and clinical operations will cause peripheral nerve damage. Clinically, when long-distance nerve defects cannot be compensated for by end-to-end sutures, grafts have to be relied on. Come bridge fix. In recent years, tissue engineering based on the principles and technologies of life science and engineering has provided a new way to construct nerve transplant substitutes. The research on using tissue engineering technology to repair nerve damage mainly focuses on the design and construction of new tissue-engineered nerve bridge devices. At present, such bridges usually use biomaterials alone, and process them into a tubular structure of nerve guide channels to serve as neural bridges. Regeneration provides proper space and guidance. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/26A61L27/24A61L27/22A61L27/20
CPCA61L2300/414A61L27/24A61L27/227A61L27/58A61L27/54A61P25/02
Inventor 杨宇民顾晓松丁斐
Owner NANTONG UNIVERSITY
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