Method for separating sweet tea polysaccharide by ultrafiltration membranes

An ultrafiltration membrane and a technology for sweet tea, applied in the field of separation and extraction of tea polysaccharides, can solve the problems of complex steps, high cost, energy consumption and ethanol consumption, etc., and achieve the effects of no environmental pollution, simple steps and easy operation.

Inactive Publication Date: 2010-05-19
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

This process of separating and extracting tea polysaccharides has problems such as complicated steps and high cost, low polysaccharide purity, low yield and activity, etc.
Moreover, because the method of alcohol precipitation only works on macromolecular polysaccharides, there are still a large number of small molecular oligopolysaccharides with medicinal effects that are not precipitated and remain in the solution, consuming a large amount of energy and ethanol, which increases production costs.

Method used

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  • Method for separating sweet tea polysaccharide by ultrafiltration membranes
  • Method for separating sweet tea polysaccharide by ultrafiltration membranes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 1 kg of sweet tea, crush it to 20 mesh, add ethanol:ether=1:1 mixed liquid (solid-liquid ratio is 1:10) degreasing, depigmentation and tea polyphenols and other components, filter the filter residue and add 5 times the temperature of the volume For distilled water at 80°C, cover and soak for 40 minutes and then filter; add 5 times the volume of distilled water at 85°C to the filter residue and soak for 30 minutes before filtering; add 3 times the volume of distilled water at 90°C to soak for 30 minutes Then filter; combine the extracts, centrifuge the extracts at 4000r / min for 10min, take the supernatant, and then filter with a 0.2μm microfiltration membrane. After the two-step purification process of centrifugation and filtration, the extract has preliminarily removed macromolecular substances such as fine dust, crude fiber, and colloid in the extract. The sweet tea polysaccharide extract is sequentially ultrafiltered through ultrafiltration membrane devices with...

Embodiment 2

[0025] Weigh 1 kg of sweet tea, crush it to 20 mesh, add ethanol: water = 4: 1 mixed solution (solid-liquid ratio is 1: 10), boil for 60 minutes, cool and filter; boil for the second and third times for 30 minutes to combine the extract, and recover under reduced pressure Ethanol, and the rest of the steps were the same as in Example 1 to obtain 11.35 g of a light green polysaccharide sample, the mass fraction of tea polysaccharide was 85.2%, and the yield accounted for 1.75% of the dry weight of tea leaves.

Embodiment 3

[0027] Infrared analysis test result of sweet tea polysaccharide obtained in embodiment 1 shows that, 3200-3600cm -1 At the O-H stretching vibration peak, 2790-3000cm -1 It is the C-H absorption peak, these two groups of peaks are the general characteristic absorption peaks of polysaccharides, 1750cm -1 It is the stretching vibration peak of -C=O, 1100cm -1 The stretching vibration peak of C-O-C indicates that the obtained sweet tea polysaccharide is mainly composed of pyranose rings. Infrared results such as figure 2 shown.

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Abstract

The invention relates to a method for separating sweet tea polysaccharide by ultrafiltration membranes, more particularly comprising: reflux extracting pretreated sweet tea by water or mixed solvent of water and ethanol; carrying out two-step purification process of centrifugation and filtration of a micro-filtering film on leach liquor, and then ultra-filtering by the ultrafiltration membranes with two specifications; and finally, separating the sweet tea polysaccharide and impurities by decompressing and concentrating trapped liquid. The method is simple, convenient and efficient, and has low cost, no pollution and higher purity of polysaccharide, thus being suitable for industrial production.

Description

technical field [0001] The invention relates to a method for separating and extracting tea polysaccharide from sweet tea with an ultrafiltration membrane. Background technique [0002] Sweet tea is a thorny perennial shrub belonging to the genus Rubus of the family Rosaceae, also known as Rubus sweet leaves. Its leaves are sweet and used for tea, so it is called "sweet tea". It is non-toxic, low in heat, high in sweetness and has a variety of health effects. Its medicinal properties and edible properties have been confirmed by local medicine and folk. Phlegm, cough, so the folks call it "sacred tea". [0003] Sweet tea is mainly produced in Guangxi, and is also distributed in Hunan, Yunnan, Sichuan and other places. It tastes sweet and delicious, and is widely eaten among the people. Efficacy is a new tea species with three economic values ​​of tea, sugar and medicine, and it is the focus of research and development in recent years. Research on sweet tea at home and abroa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 颜范勇刘冬青温景云李建龙常志华令狐强强
Owner TIANJIN POLYTECHNIC UNIV
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