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Method for inducing oxalate decarboxylase by coriolus versicolor cultured by corn stalk carbon source

The technology of oxalate decarboxylase and Coriolus versicolor is applied in the field of preparation of oxalate decarboxylase, can solve the problems of increased greenhouse effect, air pollution and the like, and achieves the effects of simple method, easy operation and low price

Inactive Publication Date: 2010-06-16
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the burned straw can be spread on the fields as fertilizer, the gas produced during the burning process will increase the greenhouse effect, and the smoke generated will also cause air pollution

Method used

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  • Method for inducing oxalate decarboxylase by coriolus versicolor cultured by corn stalk carbon source
  • Method for inducing oxalate decarboxylase by coriolus versicolor cultured by corn stalk carbon source
  • Method for inducing oxalate decarboxylase by coriolus versicolor cultured by corn stalk carbon source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Production of Coriolus versicolor from corn stalk hydrolyzate (the initial pH value of the medium is 5.0)

[0028] Weigh the fully ground corn stalks, add a sulfuric acid solution with a concentration of 5% at a solid-to-liquid ratio of 1g: 12ml, soak the corn stalk powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooker, and heat it at 121°C , reacted for 30 minutes, filtered to remove the corn stalk residue, and obtained the corn stalk hydrolyzate for later use. The content of reducing sugar in corn stalk hydrolyzate was determined by DNS method (glucose was used as the standard sugar), and it was stored at 4°C for future use. Add corn stalk hydrolyzate containing 1g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4...

Embodiment 2

[0031] Production of Coriolus versicolor from corn stalk hydrolyzate (the initial pH value of the medium is 5.0)

[0032] Weigh the fully ground corn stalks, add a sulfuric acid solution with a concentration of 3% at a solid-to-liquid ratio of 1g: 8ml, soak the corn stalk powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooker, and heat it at 150°C , reacted for 60 minutes, filtered to remove corn stalk residues, and obtained corn stalk hydrolyzate for later use. The content of reducing sugar in corn stalk hydrolyzate was determined by DNS method (glucose was used as the standard sugar), and it was stored at 4°C for future use. Add corn stalk hydrolyzate containing 1g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4 ·H 2 O...

Embodiment 3

[0035] Production of Coriolus versicolor from corn stalk hydrolyzate (the initial pH value of the medium is 5.0)

[0036] Weigh the fully ground corn stalks, add a sulfuric acid solution with a concentration of 8% at a solid-to-liquid ratio of 1g: 15ml, soak the corn stalk powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooker, and heat it at 100°C , reacted for 60 minutes, filtered to remove corn stalk residues, and obtained corn stalk hydrolyzate for later use. The content of reducing sugar in corn stalk hydrolyzate was determined by DNS method (glucose was used as the standard sugar), and it was stored at 4°C for future use. Add corn stalk hydrolyzate containing 1g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4 ·H 2 ...

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Abstract

The invention relates to a method for inducing oxalate decarboxylase by coriolus versicolor cultured by corn stalk carbon source, which comprises the following steps: (1) soaking crushed corn stalks in acid solution and hydrolyzing the crushed corn stalks at the temperature of 100 to 150 DEG C; (2) preparing a fermentation medium and adjusting the pH value to 4.0 to 5.0; (3) inoculating a mycelium into a liquid seed culture medium, when a white hyphal group is formed but does not float on a water surface, completely transferring the mycelium and the culture medium into a container containing sterile glass beads, vibrating and breaking the mixture, and inoculating the mixture into the fermentation medium in an inoculum size of 3 to 15 percent; and (4) culturing the mixture for 4 to 6 days, adding oxalic acid, taking out the mycelium after culturing the mixture for 0.5 to 6 days, and after freezing the mycelium with liquid nitrogen, grinding and crushing the mycelium, extracting hyphal fragments with acetic acid buffer solution, and centrifugating. The method of the invention is simple, easy in operation and low in cost; the preparation of a carbon source is strong in controllability; and the sugar content is stable.

Description

technical field [0001] The invention belongs to the field of preparation of oxalate decarboxylase, in particular to a method for inducing oxalate decarboxylase by culturing Coriolus versicolor as a carbon source from corn stalks. Background technique [0002] Oxalate decarboxylase can catalyze the decarboxylation of oxalate to form formic acid and carbon dioxide, so it has broad application prospects in the enzymatic detection of oxalate content, the degradation of oxalate in industrial wastewater, the preparation of low-oxalate-content foods, and the reduction of oxalate concentration in the human body. Coriolus versicolor is an important strain for producing oxalate decarboxylase, how to reduce its culture cost, produce mycelium in large quantities, and induce mycelium to synthesize oxalate decarboxylase is an important issue. Since carbon is the most abundant element in organisms, the carbon source is the most used in the medium and accounts for a relatively high proporti...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12R1/645
Inventor 洪枫杨光杨雪霞曹张军
Owner DONGHUA UNIV
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