Rapid diagnosis kit for pre-S1 antigens of hepatitis B viruses and method for preparing same

Abstract

The invention relates to a rapid diagnosis kit for pre-S1 antigens of hepatitis B viruses and a method for preparing the same. The rapid diagnosis kit mainly comprises hepatitis B virus pre-S1 antigen detection test paper formed by adhering a sample pad, a conjugate pad, a reaction film and a water absorbing layer to a lining card in sequence, and the test paper is put in a card box, wherein the conjugate pad is prepared by loading anti-HBs polyclonal antibodies on a glass fiber pad, and a detection carrier nitrocellulose film connected with the glass fiber pad is coated with anti-HBs monoclonal antibodies to form a detection line and simultaneously coated with recombined hepatitis B surface antigens to form a quality control line. By using the rapid diagnosis kit, when the sample to be detected contains the pre-S1 antigens and two magenta lines of the detection line and the quality control line are formed, the sample is judged to be positive; and otherwise, only one magenta line is formed, the sample is judged to be negative. The rapid diagnosis kit is used for detecting the pre-S1 antigens of the hepatitis B viruses in human blood serum or blood plasma and has the advantages of easy operation, rapid diagnosis, good specificity and high sensitivity.

Description

technical field [0001] The invention relates to an immunodiagnostic technique, in particular to a rapid diagnostic kit for hepatitis B virus pre-S1 antigen and a preparation method thereof. For rapid detection of hepatitis B virus pre-S1 antigen in human serum or plasma. Background technique [0002] We know that hepatitis B is a worldwide disease caused by hepatitis B virus (HBV), and my country is a country with a high incidence of hepatitis B. 40-60% of the population has been infected with HBV, and 9-10% of the population is HBV surface antigen (HBsAg) carriers. Only by evaluating the significance of these more than 100 million HBsAg-positive persons as the source of infection can they be treated fairly. Participation in social activities. [0003] Through the analysis of the structure of HBV virus particles, it was found that its outer membrane protein includes three components: S, pre-S2 and pre-S1. Pre-S1 protein mainly exists on Dane granules and cast granules, an...

AI Technical Summary

Problems solved by technology

Although both are relatively accurate and sensitive for the detection of the pre-S1 antigen of hepatitis B virus, they also have the following disadvantages: (1) the detection requires the assistance of special instruments and equipment such as microplate readers and chemiluminescence detectors, which is not conducive to the implementation of on-site direct Detection; (2) The operation process is relatively c...

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