Rapid diagnosis kit for pre-S1 antigens of hepatitis B viruses and method for preparing same

Abstract

The invention relates to a rapid diagnosis kit for pre-S1 antigens of hepatitis B viruses and a method for preparing the same. The rapid diagnosis kit mainly comprises hepatitis B virus pre-S1 antigen detection test paper formed by adhering a sample pad, a conjugate pad, a reaction film and a water absorbing layer to a lining card in sequence, and the test paper is put in a card box, wherein the conjugate pad is prepared by loading anti-HBs polyclonal antibodies on a glass fiber pad, and a detection carrier nitrocellulose film connected with the glass fiber pad is coated with anti-HBs monoclonal antibodies to form a detection line and simultaneously coated with recombined hepatitis B surface antigens to form a quality control line. By using the rapid diagnosis kit, when the sample to be detected contains the pre-S1 antigens and two magenta lines of the detection line and the quality control line are formed, the sample is judged to be positive; and otherwise, only one magenta line is formed, the sample is judged to be negative. The rapid diagnosis kit is used for detecting the pre-S1 antigens of the hepatitis B viruses in human blood serum or blood plasma and has the advantages of easy operation, rapid diagnosis, good specificity and high sensitivity.

Description

technical field [0001] The invention relates to an immunodiagnostic technique, in particular to a rapid diagnostic kit for hepatitis B virus pre-S1 antigen and a preparation method thereof. For rapid detection of hepatitis B virus pre-S1 antigen in human serum or plasma. Background technique [0002] We know that hepatitis B is a worldwide disease caused by hepatitis B virus (HBV), and my country is a country with a high incidence of hepatitis B. 40-60% of the population has been infected with HBV, and 9-10% of the population is HBV surface antigen (HBsAg) carriers. Only by evaluating the significance of these more than 100 million HBsAg-positive persons as the source of infection can they be treated fairly. Participation in social activities. [0003] Through the analysis of the structure of HBV virus particles, it was found that its outer membrane protein includes three components: S, pre-S2 and pre-S1. Pre-S1 protein mainly exists on Dane granules and cast granules, an...

AI Technical Summary

Problems solved by technology

Although both are relatively accurate and sensitive for the detection of the pre-S1 antigen of hepatitis B virus, they also have the following disadvantages: (1) the detection requires the assistance of special instruments and equipment such as microplate readers and chemiluminescence detectors, which is not conducive to the implementation of on-site direct Detection; (2) The operation process is relatively complicated, the time required for detection is relatively long, and it needs to be operated by technicians who have undergone professional technical training

(3) Generally, the detection of a single serving cannot be realized

The accuracy, sensitivity, and repeatability of traditional hepatitis B detection methods gradually show many shortcomings in the infectivity of viral infection, prognosis, and efficacy judgment.

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