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A method for separating huperzine A in Melaleuca tower by foam separation method

A foam separation method and a technology for huperzine A, which are applied in the field of huperzine A in the separation of melaleuca towers by the foam separation method, can solve the problems of short processing time, inconvenient industrialization, and high cost, and achieve low cost, easy industrialization, and low energy consumption. consumption effect

Active Publication Date: 2011-12-21
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zha Shenghua, Li Xiunan, etc. used microwave-assisted extraction and ultrasonic extraction of huperzine A and other methods. Compared with traditional extraction methods, the number of solid-liquid separations is less, the processing time is short, and the operation is simple; the solvent consumption is small and the cost is low; the choice Good performance, less impurities, good energy transmission effect, etc., but the cost is high and it is inconvenient for industrialization

Method used

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  • A method for separating huperzine A in Melaleuca tower by foam separation method
  • A method for separating huperzine A in Melaleuca tower by foam separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Weigh 100 grams of melaleuca extract (commercially available, Xi'an Sanwei Biotechnology Co., Ltd.) (with 1% huperzine A content), dissolve it in 200ml of ethanol and stir evenly to obtain a dilute total alkali solution. Then total alkali dilute solution and activator sodium dodecyl sulfonate (3 grams) are dropped in the foam separation tower (Terada Pump Terada Pump Manufacturing Institute MS-S small foam separator, total capacity 2L, the same below), foam separation The column height of tower and internal diameter ratio are 14: 1, and the temperature in the control foam separation tower is 30 ℃, stirs evenly, introduces air through the bottom of the tower, and the gas flow rate of air is 120ml / min, carries out bubbling, and the foam that obtains is passed through the tower The top is discharged into the storage tank to obtain foam liquid, and the foam is collected until no foam is produced. The foam liquid was centrifuged at 10000rpm for 15 minutes, and the centrifuge...

Embodiment 2

[0036] Weigh 120 grams of Melaleuca extract (commercially available, Xi'an Sanwei Biotechnology Co., Ltd.) (with a huperzine A content of 2%), dissolve it in 250 ml of ethanol and stir evenly to obtain a dilute total alkali solution. Then total alkali dilute solution and active agent sodium dodecylbenzenesulfonate (7 grams) are poured in the foam separation tower, the temperature in the control foam separation tower is 30 ℃, introduces air through the bottom of the tower, and the gas flow rate of air is 150ml / min, carry out bubbling, and the foam obtained is discharged into the storage tank from the top of the tower to obtain a foam liquid, and the foam is collected until no foam is produced. Centrifuge the foamy liquid at 10,000rpm for 20 minutes, take the centrifuged precipitate, put it into 15ml of 10wt% NaOH solution for full reaction, stir so as to remove sodium dodecylbenzenesulfonate, filter and take the filter cake to obtain Huperzine A Crude product, huperzine A crude...

Embodiment 3

[0038] Weigh 140 grams of melaleuca extract (commercially available, Xi'an Sanwei Biotechnology Co., Ltd.) (3% huperzine A content), dissolve it in 280ml of ethanol to obtain a dilute total alkali solution. Then total alkali dilute solution and active agent sodium dodecenyl sulfonate (12 grams) are poured in the foam separation tower, the temperature in the control foam separation tower is 30 ℃, introduces air through the bottom of the tower, and the gas flow of air is 150ml / min, for bubbling, and the obtained foam is discharged from the top of the tower into the storage tank to obtain a foam liquid, and the foam is collected until no foam is produced. Centrifuge the foam liquid, centrifuge at 10000rpm for 25 minutes, take the centrifuged precipitate, then put the resulting precipitate into 24ml 10wt% NaOH solution to fully react, stir, filter, remove sodium dodecenyl sulfonate, and filter The cake obtains the Huperzine A crude product, and the Huperzine A crude product is re...

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Abstract

The invention discloses a method for separating huperzine A in a melaleuca tower by a foam separation method. The method is as follows: the melaleuca tower extract is dissolved in ethanol, and then added into the foam separation tower with an anionic surfactant, and stirred evenly Air is blown continuously from the bottom of the tower to form foam, and the foam is collected until no foam is produced. The obtained foam is discharged from the top of the tower to obtain a foam liquid. The foam liquid is centrifuged, and the centrifuged precipitate is dissolved in NaOH aqueous solution, stirred for reaction, and filtered to obtain a filter cake. The crude product of huperzine A is recrystallized with a recrystallization solvent and dried to obtain the finished product of huperzine A; the anionic surfactant is sodium alkylsulfonate, sodium alkenylsulfonate or sodium alkylbenzenesulfonate. Compared with the prior art, the present invention shortens the process route and man-hours, greatly reduces the production cost, saves time and effort, has low cost, and has stable process, low energy consumption, environmental friendliness, easy industrialization, and its process technology is very suitable for general The existing equipment of medium-sized pharmaceutical factory.

Description

(1) Technical field [0001] The invention relates to a method for separating huperzine A in a Melaleuca tower by a foam separation method. (2) Background technology [0002] Huperzine A (Huperzine A) is a reversible, highly selective, low-toxic cholinesterase inhibitor, which can increase the content of acetylcholine in the brain and has the effect of restoring and enhancing memory. It is the first treatment for benign memory disorders in my country. and Alzheimer's dementia drugs are more effective than tacrine, physostigmine and galantamine. The new Huperzine A drugs "Shuangyiping" and "Haberin" for the treatment of senile dementia in China have been successfully produced and marketed. Huperzine A also has a significant effect on the treatment of organophosphate poisoning and myasthenia gravis. Huperzine A easily penetrates the blood-brain barrier, has high oral bioavailability, long acting time, and no serious adverse reactions. It has been listed as the second-generation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D221/22
Inventor 金志敏杨振徐威陈钢
Owner ZHEJIANG UNIV OF TECH
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