Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Adenosine deaminase diagnostic reagent (kit) and adenosine deaminase activity concentration measuring method

An adenosine deaminase and reagent technology, which is applied in biochemical equipment and methods, microbial determination/inspection, color/spectral property measurement, etc., and can solve problems such as determination and difficult popularization and application

Inactive Publication Date: 2010-06-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot be determined by the visible light analyzer in general hospitals, but requires the use of a special ultraviolet light analyzer, so it is difficult to practically popularize and apply

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The adenosine deaminase diagnostic reagent of this embodiment is a single reagent, comprising:

[0038] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0039] Stabilizer 500mmol / L

[0040] Coenzyme 3mmol / L

[0041] Inosine nucleosinase 6000U / L

[0042] Ribose 1-dehydrogenase 8000U / L

[0043] Adenosine 4mmol / L

[0044] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0045] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the tested adenosine deaminase sample to the reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is about 1 minute, the detection time is about 2 minutes, and the theoretical K value is 4180.

[0046] After adding...

Embodiment 2

[0048] The adenosine deaminase diagnostic reagent of this embodiment is a double reagent, comprising:

[0049] Reagent 1

[0050] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0051] Stabilizer 50mmol / L

[0052] Coenzyme 3mmol / L

[0053] Adenosine 4mmol / L

[0054] Reagent 2

[0055] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0056] Stabilizer 500mmol / L

[0057] Inosine nucleosinase 6000U / L

[0058] Ribose 1-dehydrogenase 8000U / L

[0059] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0060] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, test adenosine deaminase sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the delay time is about 1 ...

Embodiment 3

[0063] The adenosine deaminase diagnostic reagent of this embodiment is three reagents, comprising:

[0064]Reagent 1

[0065] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Coenzyme 3mmol / L

[0068] Adenosine 4mmol / L

[0069] Reagent 2

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 500mmol / L

[0072] Ribose 1-dehydrogenase 8000U / L

[0073] Reagent 3

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Stabilizer 500mmol / L

[0076] Inosine nucleosinase 6000U / L

[0077] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0078] When measuring the activity concentration of adenosine deaminase, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwav...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an adenosine deaminase diagnostic reagent (kit) which utilizes an enzyme colorimetric method and an enzyme linking immunoassay technology, and also relates to an adenosine deaminase activity concentration measuring method as well as composition and components of the reagent, belonging to the technical field of medical test measurement. The reagent (kit) mainly comprises the following components: buffer solution, coenzyme, adenosine, inosine nucleosidase, ribose 1-dehydrogenase and stabilizing agent; a sample is mixed with the reagent according to a certain volume proportion, so that a series of enzymatic reaction is carried out; and reactant is arranged under an ultraviolet / visible light analysis instrument, and the absorbance rising speed is detected when the main wavelength is 340nm, so that the activity concentration of the adenosine deaminase can be measured and calculated.

Description

technical field [0001] The invention relates to a diagnostic reagent (box) for adenosine deaminase, and at the same time, the invention also relates to a method for measuring the activity concentration of adenosine deaminase, which belongs to the technical field of medical examination and measurement. Background technique [0002] Medical research shows that adenosine deaminase is a nucleic acid metabolizing enzyme that has an important relationship with the body's cellular immune activity. Therefore, the determination of adenosine deaminase activity is used as an important diagnostic marker for the differential diagnosis of benign and malignant hydrothorax, cerebrospinal fluid, severe combined immunodeficiency disease (SCID), acute and chronic hepatitis, liver cirrhosis, liver cancer and other diseases. [0003] The methods for determining the activity of adenosine deaminase mainly include radionuclide method, physical method and biochemical method. As far as the applicant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com