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Beta 2-adrenergic receptor stimulant detection method based on microfluidic chip

A microfluidic chip and receptor agonist technology, applied in the fields of chemistry and physical sciences, can solve the problems of inability to automate operations, expensive instruments, cumbersome operations, etc., and achieve great scientific and economic value, low reagent consumption, and sensitivity. improved effect

Inactive Publication Date: 2010-06-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

beta 2 - Detection of adrenergic receptor agonist residues From a comprehensive consideration, the enzyme-linked immunoassay method (belonging to enzyme immunoassay technology) on multi-well plates is currently the best method for detecting clenbuterol hydrochloride, but the incubation time and immune response on the orifice plate It takes a long time and cannot complete the operation automatically
Although chromatography is the final confirmation analysis method, it has the disadvantages of cumbersome operation, high cost and expensive instruments.
Gold standard immunoassay technology and biosensing technology are limited to a single assay, currently unable to achieve high throughput

Method used

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  • Beta 2-adrenergic receptor stimulant detection method based on microfluidic chip
  • Beta 2-adrenergic receptor stimulant detection method based on microfluidic chip
  • Beta 2-adrenergic receptor stimulant detection method based on microfluidic chip

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Embodiment 1

[0074] Clenbuterol hydrochloride commonly known as clenbuterol in the standard sample on the integrated chip is a typical β 2 - Rapid detection of adrenoceptor agonist substances. The antibody, blocking agent, enzyme-labeled clenbuterol hydrochloride and the mixture of clenbuterol hydrochloride are pumped from the sample solution pool 204 into the analysis channel 203 , and the washing solution and substrate are pumped into the analysis channel 203 from the buffer pool 205 . In this part of the work, PDMS membrane is used as the solid-phase adsorption carrier of the chip immunoreaction, and the competitive enzyme immunoassay is carried out in the channel. The entire analysis process is as follows: antibody coating→washing→blocking solution blocking→washing→enzyme-labeled clenbuterol hydrochloride competes with clenbuterol for immunoreaction→washing→enzyme-catalyzed substrate→laser-induced fluorescence detection. The above-mentioned processes are all carried out in the channel...

Embodiment 2

[0077] Rapid detection of clenbuterol in pig urine samples on an integrated chip. The mixture of antibody, blocking agent, enzyme-labeled clenbuterol hydrochloride and pig urine sample added with clenbuterol hydrochloride is pumped from the sample solution pool 204 into the analysis channel 203, and the washing solution and substrate are pumped into the analysis channel from the buffer pool 205 Channel 203. This part of the work also uses PDMS membrane as the solid-phase adsorption carrier of the chip immunoreaction, and performs competitive enzyme immunoassay in the channel. The entire analysis process is as follows: antibody coating→washing→blocking solution blocking→washing→enzyme-labeled clenbuterol hydrochloride competes with clenbuterol for immunoreaction→washing→enzyme-catalyzed substrate→laser-induced fluorescence detection. The above-mentioned processes are all carried out in the channel of the microfluidic chip, and are controlled by a pneumatic micropump and microv...

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Abstract

The invention discloses a Beta 2-adrenergic receptor stimulant detection method based on microfluidic chip; the detection system adopts laser detection; the detection system takes the microfluidic chip (1) as the core, and the microfluidic chip (1) is sequentially compounded through three-layer structure, including: the upper fluid pathway layer (101), a middle elastic film layer (102) and a lower pneumatic control layer (103); the relationship of the three parts includes that: the upper fluid pathway layer (101) and the lower pneumatic control layer (103) are tightly connected with the elastic film layer (102) through surface tension respectively between the two layers and the elastic film layer. The invention has the advantages of short detection time, low LOD, good repeatability, easy operation, less sample dosage and integration, also has great foreseeable scientific value and economic value.

Description

technical field [0001] The present invention relates to chemical and physical sciences, in particular to chip immunoassay technology; in particular, a microfluidic chip-based β 2 - Adrenergic receptor agonist detection system. Background technique [0002] beta 2 - Adrenergic receptor agonist drugs are a class of artificially synthesized drugs, mainly used to prevent and treat bronchial asthma and bronchospasm in humans and animals. beta 2 - Adrenergic receptor agonists can be used to strengthen the muscles of athletes and animals (such as horses) in sports competitions, and improve sports performance. The International Olympic Committee will beta 2 - Adrenergic receptor agonists are listed as prohibited drugs. In the 1980s, research at home and abroad showed that adding β to feed 2 - Adrenergic receptor agonists have a nutrient redistribution effect, which can significantly increase lean meat percentage. Humans consume beta- 2 - Adrenergic receptor agonist drugs have...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/557G01N21/64
Inventor 林炳承孔静杜昱光姜雷秦建华
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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