Acidophilus milk for infants and preparation method thereof
A technology for infants and young children, yogurt, applied in the direction of milk preparations, dairy products, applications, etc., can solve the problem of not being suitable for direct consumption by infants and young children
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Embodiment 1
[0039] 1. Selection of fermentation strains and preparation of medium
[0040] Fermentation strain - Bifidobacterium longum CICC 6188 Bifidobacterium longum.
[0041] Medium: gravy 10g / L, peptone 5g / L, yeast extract 3g / L, glucose 5g / L, starch 1g / L, NaCl 5g / L, sodium acetate 3g / L, L-cysteinium chloride 0.5g / L , agar 0.5g / L, 121 ℃ damp heat sterilization for 15min.
[0042] 2. Preparation process of fermentation strain
[0043] 2.1 Activation of freeze-dried bacteria powder
[0044] Measure 10ml of 0.9% physiological saline into 2 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacteria powder in the ampoule of fermented bacteria into 0.9% physiological saline in a sterile state Shake it in salt water to dissolve it, and activate it in a 30°C incubator for 30 minutes, and set aside.
[0045] 2.2 Expanded cultivation of fermentation strains
[0046] 2.2.1 Preparation of primary fermented seeds
[0047] Measure 200ml of fermentation ...
Embodiment 2
[0070] 1. Selection of fermentation strains and preparation of medium
[0071] Fermentation strain - Bifidobacterium longum CICC 6188 Bifidobacterium longum.
[0072] Medium: gravy 10g / L, peptone 5g / L, yeast extract 3g / L, glucose 5g / L, starch 1g / L, NaCl 5g / L, sodium acetate 3g / L, L-cysteinium chloride 0.5g / L , agar 0.5g / L, 121 ℃ damp heat sterilization for 15min.
[0073] 2. Preparation process of fermentation strain
[0074] 2.1 Activation of freeze-dried bacteria powder
[0075] Measure 10ml of 0.9% physiological saline into 2 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacteria powder in the ampoule of fermented bacteria into 0.9% physiological saline in a sterile state Shake it in salt water to dissolve it, and activate it in a 30°C incubator for 30 minutes, and set aside.
[0076] 2.2 Expanded cultivation of fermentation strains
[0077] 2.2.1 Preparation of primary fermented seeds
[0078] Measure 200ml of fermentation ...
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