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Method for testing formaldehyde in food by means of derivation extraction

An extraction method and formaldehyde technology, which is applied in the field of liquid chromatography for the determination of formaldehyde in food, to achieve the effects of improving detection efficiency, inhibiting release, improving stability and reproducibility

Inactive Publication Date: 2010-06-30
THE INSPECTION & QUARANTINE TECH CENT ZHEJIANG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in actual operation, there are certain difficulties in the selection of blank matrix, the preparation of formaldehyde calibration work curve, and the comparison of results between different laboratories.

Method used

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  • Method for testing formaldehyde in food by means of derivation extraction
  • Method for testing formaldehyde in food by means of derivation extraction
  • Method for testing formaldehyde in food by means of derivation extraction

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Embodiment 1

[0063] A detailed description will be given below of specific embodiments of the present invention.

[0064] 1.1 Method summary

[0065] Direct extraction of formaldehyde from derivatized samples. After liquid-liquid extraction and purification, it was determined by liquid chromatography at a wavelength of 365nm, and quantified by external standard method.

[0066] 1.2 Reagents and materials

[0067] Unless otherwise stated, all reagents used were of analytical grade. The water used was ultrapure water cooled after boiling for 10 min.

[0068] 1.2.1 Acetonitrile: chromatographically pure.

[0069] 1.2.2 n-Hexane: chromatographically pure.

[0070] 1.2.3 Ammonium sulfate.

[0071] 1.2.4 Sodium acetate.

[0072] 1.2.5 Glacial acetic acid

[0073] 1.2.6 Buffer solution (pH 5): Weigh 2.64g of sodium acetate, dissolve it in an appropriate amount of water, add 1.0mL of glacial acetic acid, and dilute to 500mL with water.

[0074] 1.2.7 2,4-Dinitrophenylhydrazine: purity ≥99...

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Abstract

The invention relates to a method for testing the content of formaldehyde in food by means of liquid chromatography, in particular to a method for testing the formaldehyde in import and export aquatic products, vegetables (including mushroom), cereal products, dairy products and beers, etc. The method for testing the content of the formaldehyde in the food by means of liquid chromatography comprises the steps of: (1) extracting; (2) purifying; (3) preparing formaldehyde standard work solution; (4) testing; (5) blank testing; and (6) computing and expressing a result. The method adopts acetonitrile / pH 5 ethylic acid buffer solution (1 / 1, v / v) of 2, 4-dinitrophenylhydrazine to directly derive and extract the formaldehyde in a sample and test by means of the liquid chromatography. The formaldehyde is extracted from the sample under water bath at the temperature of 60 DEG C and the derivatization reaction is performed. The 2, 4-dinitrophenylhydrazine and the group in protein compete for the formaldehyde in the reaction solution, thereby being capable of effectively preventing the formaldehyde from combining with the protein in the sample and having higher formaldehyde recovery ratio than that of by using an immersion method.

Description

technical field [0001] The invention relates to a method for determining formaldehyde in food by liquid chromatography, in particular to a method for determining formaldehyde in imported and exported aquatic products, vegetables (including shiitake mushrooms), cereal products, dairy products, beer and the like. Background technique [0002] Formaldehyde, commonly known as formalin, is a colorless, irritating gas with a strong odor, and its 35% to 40% aqueous solution is commonly known as formalin. Formaldehyde is a protoplasmic poison, which can be combined with protein. After inhalation of high concentration formaldehyde, severe irritation and edema of the respiratory tract, eye stinging, headache, and bronchial asthma may occur. Direct skin contact with formaldehyde can cause dermatitis, stains, and necrosis. Frequent inhalation of a small amount of formaldehyde can cause chronic poisoning, mucous membrane hyperemia, skin irritation, allergic dermatitis, nail keratosis an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 吕春华陈笑梅刘海山史颖珠谢文黄超群朱晓雨
Owner THE INSPECTION & QUARANTINE TECH CENT ZHEJIANG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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