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A method for preparing hollow microcapsules based on the specific interaction between concanavalin and glycogen

A technology of concanavalin and microcapsules is applied in the field of preparing hollow microcapsules based on the specific interaction between concanavalin and glycogen, which can solve the problems of difficult to find pH environment, easy inactivation, high price, etc. Good application prospects, good biocompatibility and degradability, low cost of raw materials and stable effects

Inactive Publication Date: 2011-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is based on the interaction between phenylboronic acid groups and sugars, but most of the glucose responsiveness of this type of capsule can be more obvious when pH>9, but it is difficult to find such a pH environment in the human body
The other is to use glucose oxidase to oxidize glucose molecules to cause changes in the pH value of the local microenvironment to affect the capsule’s permeability to achieve the glucose response of the capsule, but the raw material-enzyme is expensive and easily inactivated

Method used

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  • A method for preparing hollow microcapsules based on the specific interaction between concanavalin and glycogen
  • A method for preparing hollow microcapsules based on the specific interaction between concanavalin and glycogen
  • A method for preparing hollow microcapsules based on the specific interaction between concanavalin and glycogen

Examples

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Effect test

example 1

[0025] 1) Add CaCl to the tris-hydrochloric acid (Tris-HCl) buffer solution with a pH value of 7.8 and a concentration of 0.05 mol / L 2 with MnCl 2 , so that the buffer contains 1mmol / L Mn 2+ and 1mmol / L Ca 2+ , using the buffer as a solvent to prepare a concanavalin solution with a concentration of 0.2 mg / ml and a glycogen solution with a concentration of 1 mg / ml;

[0026] 2) Add 40 mg of calcium carbonate colloidal particles to 4 ml of a polyethyleneimine solution with a concentration of 4 mg / ml, a pH value of 7, and 0.5 M NaCl, and centrifuge and wash with water after oscillating and absorbing;

[0027] 3) Disperse the particles obtained in step 2) in the concanavalin solution of step 1), oscillate for adsorption and centrifuge wash with tris-hydrochloric acid buffer solution with a concentration of 0.025 mol / L to obtain a surface-assembled layer particles of concanavalin molecules;

[0028] 4) Disperse the particles obtained in step 3) in the glycogen solution of step 1...

example 2

[0031] The steps are the same as in Example 1, but the buffer pH condition is 7.1 instead of 7.8 in all steps. The optical microscope photo of the obtained microcapsules in wet state is shown in figure 2 .

example 3

[0033] The procedure is the same as in Example 1, but in step 3), fluorescein isocyanate (FITC)-labeled concanavalin is used instead of common concanavalin. Fluorescence micrographs of the obtained microcapsules in a wet state are shown in image 3 .

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Abstract

The invention discloses a method for preparing hollow microcapsules based on the specific interaction between concanavalin and glycogen. This method uses the specific recognition of concanavalin and glucose units contained in glycogen to form a multi-layer film on the surface of carbonate colloidal particles by layer-by-layer assembly, and further uses disodium ethylenediaminetetraacetic acid complexation to remove Carbonate colloidal particles to obtain hollow microcapsules. The microcapsules prepared by this method can produce specific responses to specific monosaccharides and polysaccharides under physiological pH conditions, and have the advantages of low raw material cost, good biocompatibility, biodegradability, etc., and have good application in the field of biomedicine Application prospect.

Description

technical field [0001] The invention relates to a method for preparing hollow microcapsules, in particular to a method for preparing hollow microcapsules based on the specific interaction between concanavalin and glycogen. Background technique [0002] Microcapsules are a specific three-dimensional structure formed by separating the space inside the capsule from the space outside the capsule through film-forming substances. Its shape is mainly spherical, and it can also be oval, square or rectangular, polygonal and other irregular shape. The size of traditional microcapsules is usually in the order of microns to millimeters, and the wall thickness is from submicron to hundreds of microns. The capsule wall is usually composed of natural or synthetic polymer materials, or inorganic compounds. Microcapsules have very important applications in food, medicine, cosmetics, biomedicine and other fields. [0003] There are many methods for the preparation of microcapsules. Accordi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/14
Inventor 高长有朱一仝维鋆
Owner ZHEJIANG UNIV
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