Skin firming and lifting compositions and methods of use
A composition and skin technology, applied in skin care preparations, drug combinations, skin diseases, etc., can solve skin inflammation and allergies, prevent bacterial growth, skin aging, etc.
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Embodiment 1
[0050] Example 1: Analysis of extracellular matrix synthesis in vitro.
[0051] Human Hs27 fibroblasts and HEK001 keratinocytes were purchased from ATCC (Manassas, VA). The co-cultures (Co-cultures) were cultured in 24-well plates at a ratio of 1:4 respectively. After the incubation period, the medium was changed and the cells were exposed to test samples (0.1% or 1% v / v) and incubated overnight. The test sample contains: DOT-9002-14A; Satsuma citrus extract; seaweed (Pullulan and / or Nannochloropsisoculata) (eg - TIGHT); Blacktop Algae extract (eg Scopariane); Chlorella extract (eg Dermochlorella); Pea extract; Bamboo extract;
[0052] After overnight incubation with the test samples, supernatants were collected from the cell cultures and analyzed for the presence of α-elastin, procollagen and hyaluronic acid. Elastin was obtained using Fastin from Biocolor, Inc. (Newtownabbey, Northern Ireland) TMElastin Kit to analyze. See Liao J., Vesely I. 2004. "Relationship betwee...
Embodiment 2
[0054] Example 2: Evaluation of skin lifting benefits.
[0055] A three week controlled clinical study was conducted to evaluate the performance and efficacy of the composition of the present invention comprising lifting serum. Specifically, the lifting serum formulations tested in this three week study contained the following ingredients (all percentages reported below are based on the total weight of the composition):
[0056] Medium: water
[0057] Emulsifier: a composition comprising hydrogenated lecithin, sodium methyl stearoyl aurate, glycerin, squalane, hydroxypropylmethylcellulose stearyl ether, and vegetable sorbitan stearate ester
[0058] Skin Conditioners: Caffeine; Dipotassium Glycyrrhizinate; Panthenol; Ethoxydiglycol; Proline; Contains C12-15 Alkyl Benzoate, Dipropylene Glycol Dibenzoate, PPG-15 Stearyl Composition of ether benzoate; caprylic / caprylic triglyceride; di-C12-15 alkyl fumarate; cetyl alcohol; combination comprising polyacrylate 13, polyisobutylen...
Embodiment 3
[0092] Example 3: Irritation, Acnegenicity and Comedogenicity of Skin Firming Products
[0093] A six-week clinical use study was conducted to determine the irritative, comedogenic and comedogenic potential of the skin firming product. Fifty-four female test subjects had self-perceived acne-prone facial skin combined with dry skin, and they completed the study with regular twice-daily use of moisturizer on the face.
[0094] At the baseline visit, subjects were administered in-clinic exaggerated amounts of the test material to the ocular region (approximately 0.5 g). During this study, formulations comprising the compositions of the present invention were applied twice daily to the face and neck of the test subjects for the duration of the study. Clinical evaluations were performed at Baseline, Second Administration of Baseline Visit, Week 3 and Week 6. The inventive formulations measured in this study are disclosed in Example 2.
[0095] As indicated below, at each visit t...
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