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Auxiliary pipeline system supporting operation of bioreactor group and operation process thereof

A bioreactor and pipeline system technology, applied in the field of microalgae biology, can solve the problems of backward support of the pipeline system, and achieve the effects of reducing light shortage, reducing costs, and reducing light inhibition

Active Publication Date: 2012-10-17
云南爱尔发生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problem of backward pipeline system support in the closed photobioreactor in the large-scale cultivation of microalgae, the purpose of the present invention is to provide an auxiliary pipeline system and its operation for supporting the operation of the bioreactor group in the microalgae cell engineering culture Technology, combined with actual practical experience, through continuous experiments, using the principle of siphon and the law of natural movement of liquid from high position to bottom position under the action of gravity of the earth, successfully constructed a simplified pipeline number, and at the same time got rid of the need to rely on the pump to drive the liquid flow The new accessory pipeline system can support the expansion and healthy operation of the bioreactor group in the microalgae cell engineering culture

Method used

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  • Auxiliary pipeline system supporting operation of bioreactor group and operation process thereof
  • Auxiliary pipeline system supporting operation of bioreactor group and operation process thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] 120-ton scale parallel columnar reactor piping system

[0047] In the standardized plastic greenhouse (length 66m, width 10m, net area 1 mu of land), according to figure 1 The piping system of parallel columnar reactors was designed by means of the method, and a total of four pipes were installed with a volume of 120 tons.

[0048] First, in the supply and recovery system at one end outside the greenhouse, separate the algae pipeline (diameter 4cm), mixed gas pipeline (diameter 6.5cm), culture solution pipeline (diameter 6.5cm), and algae liquid harvesting pipeline (diameter 9.0cm) four, each of the above-mentioned pipelines is respectively connected with the corresponding pipelines of the parallel columnar reactor pipeline system in each greenhouse workshop: the algae pipeline 12, the mixed gas pipeline 10, the culture solution pipeline 11, and the total discharge pipeline 18. The mixed gas refers to air and carbon dioxide, wherein the volume content of carbon dioxide...

Embodiment 2

[0067] like figure 2 As shown, the difference from Example 1 is that this example takes two flat-plate reactor units as an example, and different numbers of units (1, 2, 3...n units) form a reactor group.

[0068] The results of the examples show that the present invention utilizes the principle of siphon and the law of natural movement of liquid from high to low under the action of the earth's gravitational force to effectively seal and connect different pipelines together, and rely on related switches to block or control the flow of liquid or gas in the pipeline. , Through the standardized operation process, the relevant switches are effectively turned on or off to realize the connection or blockage of the corresponding pipelines, and ensure that the pipelines are fully flushed after use, reduce the amount of residual cells in the pipelines, and avoid cross-contamination. The implementation of the present invention gets rid of the dependence on the water pump in the transfe...

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Abstract

The invention relates to an auxiliary pipeline system used for supporting the operation of key equipment of a bioreactor group and an operation process thereof in the microalgae cell engineering culture, which belongs to the technical field of microalgae organism. The pipeline system of the invention consists of a pipeline group and a switch group, wherein the pipeline group comprises a culture liquid pipeline, a gas pipeline, an algae seed pipeline and a material discharge pipeline, the switch group comprises a culture liquid pipeline switch, a algae seed pipeline switch, a liquid pipeline switch, a gas pipeline switch, a material feeding branch pipeline switch, a reactor unit switch, a material discharge branch pipeline switch and a material discharge pipeline total switch. The invention effectively seals and connects different pipelines, the flowing direction of the liquid or the gas in the pipelines is blocked or controlled through the relevant switches, the relevant switches can be effectively switched on or switched off through the standard operation flow process, the communication or the blockage of correspondingly pipelines can be realized, the sufficient flushing of the pipelines after being used is ensured, the cell remaining quantity in the pipelines is reduced, the cross contamination is avoided, and the invention can be used for continuous and semi-continuous cellengineering culture.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and relates to an auxiliary pipeline system used to support the operation of a bioreactor group of key equipment in the engineering culture of microalgae cells and an operating process thereof. Background technique [0002] Microalgae are important biological resources for the survival and development of human society in the future. First of all, microalgae are an important part of primary productivity in the biosphere, and are the only bait or bait for bait organisms in the entire growth process (or specific developmental stages) of aquatic animals in the aquatic ecosystem. At the same time, the number of microalgae species is close to 50% of plant species, and has diversity, complexity and particularity in terms of germplasm, ecological distribution, genetic information, biochemical composition, and metabolic pathways, which further determine its potential nutritional and pharmaceutical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M21/02C12M23/58
Inventor 刘建国张勇梁文伟
Owner 云南爱尔发生物技术股份有限公司