Detoxication and tissue culture rapid propagation method of chewing cane axillary buds

A technology of tissue culture rapid propagation and fruit cane, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of pollution, incomplete virus removal, low survival rate, etc., and achieve high survival rate and fast speed , The effect of easy inoculation operation

Inactive Publication Date: 2010-08-18
GUANGZHOU SUGARCANE IND RES INST
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The problem that the shoot tip meristem detoxification method exists is: to cut out very tiny (0.2mm below) shoot tip meristem, need to operate under a microscope, has certain difficulty; The higher the rate, the lower the survival rate, and the cut shoot tip tissue is too l

Method used

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  • Detoxication and tissue culture rapid propagation method of chewing cane axillary buds

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[0023] Example 1

[0024] A method for detoxifying tissue culture and rapid propagation of fruit cane is carried out as follows:

[0025] 1) Selection and sterilization of explants: From July to November, take the axillary buds of fruit cane, soak them in 75% alcohol for 0.5 to 1.0 minutes, then treat them with 0.1% mercury solution for 3 to 4 minutes, then rinse with sterile water for 3 ~5 times.

[0026] 2) Axillary bud induction culture: The axillary buds treated in step 1) are harvested under aseptic conditions, 2~3mm axillary buds are picked, the leaf sheaths are carefully removed, and the axillary bud induction medium (MS+6-BA 0.5mg / L+ IBA 0.02mg / L+activated carbon 1g / L+sucrose 30g / L+arginine 100mg / L+inositol 100mg / L+agar 5g / L) to induce bud formation.

[0027] 3) Differentiation culture: transfer the induced buds formed in step 2) to differentiation medium (MS+TDZ 0.003mg / L+IBA 0.02mg / L+sucrose 30g / L+arginine 100mg / L+ inositol 100mg / L+agar 5g / L) cluster buds are induced.

[0...

Example Embodiment

[0033] Example 2

[0034] A method for detoxifying tissue culture and rapid propagation of fruit cane is carried out as follows:

[0035] 1) Selection and sterilization of implants: From July to November, take the axillary buds of fruit cane, soak them in 75% alcohol for 0.5 to 1.0 minutes, then treat them with 0.1% mercury solution for 3 to 4 minutes, then rinse with sterile water for 3 ~5 times.

[0036] 2) Axillary bud induction culture: the axillary buds treated in step 1) are harvested under aseptic conditions, 2~3mm axillary buds are picked, the leaf sheaths are carefully removed, and the axillary bud induction medium (MS+6-BA 0.8mg / L+IBA 0.04mg / L+active charcoal 1.8g / L+arginine 150mg / L+inositol 130mg / L) to induce bud formation.

[0037] 3) Differentiation culture: transfer the induced buds formed in step 2) to differentiation medium (MS+TDZ 0.008mg / L+IBA0.04mg / L+sucrose 35g / L+arginine 120mg / L+inositol 130mg / L) , Induce clump buds.

[0038] 4) Proliferation culture: cut the clu...

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Abstract

The invention relates to the technical field of the detoxication and the tissue culture of sugarcane and provides a detoxication and tissue culture rapid propagation method of chewing cane, characterized by comprising the following steps of: (1) selecting and sterilizing plants; (2) carrying out the induced culture of axillary buds; (3) carrying out differentiation culture; (4) carrying out propagation culture; (5) carrying out rooting culture; (6) transplanting; and (7) detecting viruses (germs). The detoxication and tissue culture rapid propagation method of chewing cane is characterized in that a differentiation culture medium formula in the step (3) comprises the following components: 0.001-0.01mg/L of MS and TDZ, 0.02-0.05 mg/L of IBA, 30-40 g/L of cane sugar, 100-150 mg/L of arginine and 100-150 mg/L of inositol. The invention uses chewing cane axillary buds as explants, is easy to perform vaccination operation, and reaches the high survival rate over 90 percent. By the adopted differentiation culture medium formula, cluster buds can be rapidly differentiated from induced buds in the differentiation culture process. The invention can rapidly culture chewing cane detoxication buds and multiply a monthly propagation coefficient by 5-10 times, thereby being suitable for large-scale factory production.

Description

Technical field [0001] The invention relates to the technical field of sugarcane detoxification tissue culture. Background technique [0002] Sugarcane (including fruit cane) ratoon stunting disease has been widespread in the main sugarcane producing areas of China and sugarcane areas around the world for many years, resulting in the degradation of varieties and seriously affecting the quality and yield of sugarcane. [0003] There are many methods to obtain virus-free (bacteria) plants of sugarcane, such as heat treatment detoxification (bacteria), shoot tip culture detoxification (bacteria), callus culture detoxification and combination methods. Among them, the better method generally used is the shoot tip culture detoxification (bacteria) method. The specific steps are: cut the shoot tip (meristem below 0.2mm) from the apical meristem of the sugarcane plant, induce shoots, and grow into small plants Obtain a virus-free vaccine. The problem with the method of detoxification of ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈仲华刘睿陈月桂杨湛端沈万宽谭嘉娜
Owner GUANGZHOU SUGARCANE IND RES INST
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