Method for extracting oridonin from rabdosia rubescens and purifying oridonin

Abstract

The invention discloses a novel process for extracting oridonin from rabdosia rubescens and purifying the oridonin. In the process, extraction is performed in 60 to 70 percent ethanol for 2 to 3 times, active carbon is used for decolorization, the extract is condensed under reduced pressure till the concentration of the methanol is 20 to 30 percent, macroporous resin absorption is performed, 75 to 85 percent methanol is used for elution, condensation is performed, extraction is performed step by step by using petroleum ether and ethyl acetate, ethyl acetate extracting solution is recovered, a small amount of petroleum ether is added, the solution is stood for crystallization, and refluxing and saturated dissolution and crystallization in 70 percent methanol and 90 percent methanol are performed in turn. The process is simple and is easy in operation.

Description

Technical field:

[0001] The invention relates to a method for extracting and purifying oridonin from Rubescens sativa. The method is a method for purifying oridonin by adopting low-alcohol state resin adsorption, extraction and crystallization. Background technique:

[0002] Oridonin is also called Oridonin and Yanming Caoning.

[0003] Molecular formula: C20H28O6

[0004] Molecular weight: 364.43

[0005] Physical properties: melting point 248°C-250°C, slightly soluble in water, soluble in methanol, ethyl acetate, acetone, etc.

[0006] Oridonin A is a tetracyclic diterpenoid isolated from the traditional Chinese medicine Oridonin. The role of oridonin in cancer treatment has attracted people's attention, and it has been found that it has inhibitory effects on various cell lines such as lung cancer, prostate cancer, and breast cancer.

[0007] Donglingcao, also known as Binglingcao, Yanmingcao, Caihuacao, etc., is a perennial herb or subshrub of Lamiaceae Fragrant Tea. ...